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The 2020 Attune NxT Flow Cytometer continues to offer more options, increased flexibility, and software updates that further improve performance, reliability, and robustness. Since its initial unveiling, the compact system with innovative acoustic technology is moving ahead with added new functionalities and capabilities making multiparametric flow cytometry available to both new and experienced researchers. Experience the difference acoustic assisted hydrodynamic focusing technology brings to your workflow.
Please see our SARS-CoV-2 Decontamination Guidance for Thermo Fisher Scientific Instrumentation
Request a demo Request a quote 3D Demo
Designed for faster experimental run times, the Invitrogen Attune NxT compact flow cytometer offers rapid and accurate analysis for a broad range of sample types. Experience the difference.
The 40-page Flow Cytometer Evaluation Guide will enable a better understanding of the components and capabilities of various flow cytometers for objective comparison of instruments from several manufacturers.
Improve your workflow for fully automated sample acquisition from 96-well and 384-well microtiter plates. There are no physical changes to the system needed in order to run plate based experiments—everything is controlled in the software.
This grow-with-you system can be customized with up to 4 lasers and 14 colors and now up to 6 channels off of the violet laser. The optical system was designed to provide optimal signal and has the range to detect very bright as well as dim signals. The Attune NxT Flow Cytometer allows researchers the freedom to build multicolor panels, accommodating common fluorophores and dyes. Build your multicolor panel for the Attune NxT flow cytometer with our superior reagents and antibodies.
Lasers | Laser configuration Cat. No. | Lasers included, No. of detection channels | Total detection channels* | ||||
---|---|---|---|---|---|---|---|
Violet, 405 nm | Blue, 488 nm | Yellow, 561 nm | Green, 532 nm | Red, 637 nm | |||
1 | Blue A24864 A24864CFR** | Available as upgrade† |
4
| Available as upgrade† | Available as upgrade† | Available as upgrade† | 6 |
2 | Blue/green A28995 A28995CFR** | Available as upgrade† |
3
|
4
| Available as upgrade† | 9 | |
Blue/yellow A24861 A24861CFR** | Available as upgrade† |
3
|
4
| Available as upgrade† | 9 | ||
Blue/red A24863 A24863CFR** | Available as upgrade† |
4
| Available as upgrade† | Available as upgrade† |
3
| 9 | |
Blue/violet A24862 A24862CFR** |
4
|
4
| Available as upgrade† | Available as upgrade† | Available as upgrade† | 10 | |
Blue/violet 6 A29002 A29002CFR** |
6
|
3
| Available as upgrade† | Available as upgrade† | 11 | ||
3 | Blue/green/red A28997 A28997CFR** |
Available as upgrade†
|
3
|
4
|
3
| 12 | |
Blue/red/yellow A28993 A28993CFR** | Available as upgrade† | 3 | 4 | 3 | 12 | ||
Blue/green/violet A28999 A28999CFR** |
4
|
3
|
4
| Available as upgrade† | 13 | ||
Blue/violet/yellowA24859 A24859CFR** |
4
|
3
|
4
| Available as upgrade† | 13 | ||
Blue/red/violet A24860 A24860CFR** |
4
|
4
| Available as upgrade† | Available as upgrade† |
3
| 13 | |
Blue/red/violet 6 A29003 A29003CFR** |
6
|
3
| Available as upgrade† |
3
| 14 | ||
4 | Blue/red/violet/green A29001 A29001CFR** |
4
|
3
|
4
|
3
| 16 | |
Blue/red/yellow/violet A24858 A24858CFR** |
4
|
3
|
4
|
3
| 16 | ||
Blue/red/yellow/violet 6 A29004 A29004CFR** |
6
|
2
|
3
|
3
| 16 | ||
* Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel. † Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser Upgrade, Red Laser Upgrade, or Green Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options. ** Instrument package comes with a 21 CFR part 11 Software License and an Attune NxT software license. |
A specification is derived from a specific test or calculation, but these tests aren’t standardized across instrument developers and may be misleading in a side-by-side comparison because their values may have been calculated in different ways, despite sharing the same terminology. This 8-page Comparing Technical Specification Documents resource offers helpful tips on deciphering variations.
Optics |
| ||||||||||||||||||||||||||||
Fluidics |
| ||||||||||||||||||||||||||||
Performance |
| ||||||||||||||||||||||||||||
Software |
| ||||||||||||||||||||||||||||
Quality and regulatory |
| ||||||||||||||||||||||||||||
Data management |
| ||||||||||||||||||||||||||||
Installation requirements |
|
Invitrogen Attune NxT Software is designed to provide powerful data acquisition and analysis using an intuitive, user-friendly interface. The instrument software features and benefits include:
Since its initial unveiling, the compact Attune NxT Flow Cytometer continues to offer more options and added functionality with additional lasers, more detection channel options, sample handling and robotic solutions.
Find laser upgrades, optional layout conversion kits, autosampler upgrades, robotics arm options, as well as filter kits and accessory parts.
Attune NxT upgrades | Filter | Common fluorochromes | Cat. No. |
---|---|---|---|
Attune NxT Yellow Laser Upgrade Kit | 585/16 | PE, Alexa Fluor 532, Alexa Fluor 568 | 100022779 |
620/15 | Alexa Fluor 594, PE-Texas Red, PE-Alexa Fluor 610 | ||
695/40 | PE-Alexa Fluor 700, TRI-COLOR (PE-Cy5), PE-Cy5.5, Qdot 705 | ||
780/60 | Qdot 800, PE-Cy 7, PE-Alexa Fluor 750 | ||
Attune NxT Red Laser Upgrade Kit | 670/14 | APC, Alexa Fluor 647, Qdot 655 | 100022778 |
720/30 | Alexa Fluor 680, Alexa Fluor 700, APC-Alexa Fluor 700, Qdot 705 | ||
780/60 | APC-Alexa Fluor 750, APC-Cy7, APC-eFluor 780, Qdot 800 | ||
Attune NxT Green Laser Upgrade Kit | 575/36 | PE, Alexa Fluor 532, Alexa Fluor 555, DsRed | A32701 |
620/15 | Alexa Fluor 568, Alexa Fluor 594, Qdot 605, PE-Texas Red, PE-Alexa Fluor 610 | ||
695/40 | Qdot 705, PE-Alexa Fluor 700, TRI-COLOR, PE-Cy5.5 | ||
780/60 | Qdot 800, PE-Cy7 | ||
Attune NxT Violet Laser Upgrade Kit | 440/50 | Super Bright 436, Alexa Fluor 405, eFluor 450, Pacific Blue | 100022777 |
512/25 | eFluor 506, Pacific Green | ||
603/48 | Super Bight 600, Pacific Orange, Qdot 605 | ||
710/50 | Super Bright 702, Qdot 705 | ||
Attune NxT Violet 6 Conversion Kit, Blue Laser | 530/30 | Alexa Fluor 488, FITC | A35428 |
574/26 | PE, PE-Alexa Fluor 610, Alexa Fluor 546 | ||
695/40 | PE-Alexa Fluor 700, PE-Cy5.5, PerCP, PerCP-Cy5.5, Qdot 705, TRI-COLOR | ||
Attune NxT Violet 6 Conversion Kit, Violet Laser | 450/40 | Super Bright 436, eFluor 450, Pacific Blue | A36569 |
525/50 | eFluor 506, Pacific Green, eFluor 506 | ||
610/20 | Super Bright 600, Qdot 605, Pacific Orange | ||
660/20 | Super Bright 645 | ||
710/50 | Super Bright 702, Qdot 705 | ||
780/60 | Super Bright 780 | ||
Attune NxT Violet 6 Conversion Kit, Red Laser | 670/14 | APC, Alexa Fluor 647, Qdot 655 | A36571 |
720/30 | Alexa Fluor 680, Alexa Fluor 700, APC-Alexa Fluor 700, Qdot 705 | ||
780/60 | APC-Alexa Fluor 750, APC-eFluor 780, APC-Cy7, Qdot 800 | ||
Attune NxT Violet 6 Conversion Kit, Yellow Laser | 585/16 | PE, Alexa Fluor 532, Alexa Fluor 568 | A36572 |
620/15 | Alexa Fluor 594, PE-Texas Red, PE-Alexa Fluor 610 | ||
695/40 | PE-Alexa Fluor 700, TRI-COLOR, PE-Cy5.5, Qdot 705 | ||
780/60 | Qdot 800, PE-Cy 7, PE-Alexa Fluor 750 |
NOTE: One accessory kit for each laser on your instrument needs to be purchased to successfully complete V6 instrument conversion.
The Invitrogen Attune NxT Flow Cytometer was developed with acoustic-assisted hydrodynamic focusing technology, and advanced fluidics designed to minimize clogging and effectively handle a broad range of cell types. This allows for a higher degree of data, detail, and throughput that enables processing of a large range of sample types, including large clumpy cells, samples with a low concentration of cells, and precious samples, more quickly and accurately than ever before with no loss in data quality.
Instantly search over 475 peer-reviewed scientific publications featuring the Invitrogen Attune NxT Flow Cytometer.
At Thermo Fisher Scientific, we are proud to receive the 2019 CiteAb award for the Attune NxT Flow Cytometer. CiteAb's Flow Cytometer to Watch in 2019 award was based on the number of published citations for the instrument.
The following applications were performed using the Attune NxT Flow Cytometer.
The Attune NxT Flow Cytometer enables well-separated bacterial populations. For researchers working with E. coli, the available green 532 nm laser delivers distinct separation of double-live populations of dividing cells (green) and dead (red) E. coli cells (Figures 1 and 2).
Figure 1. Staining of E. coli cells using the Invitrogen BacLight LIVE/DEAD Bacterial Viability Kit and blue laser excitation of Invitrogen SYTO 9 dye and propidium iodide (PI). Samples were analyzed on the Attune NxT Flow Cytometer at the 12.5 μL/min flow rate with an event rate of approximately 5,000 events/second. Instrument settings (voltages, threshold, and advanced settings) were set using single-color controls. The blue 488 nm laser was used for fluorescence excitation of both SYTO 9 dye and PI. SYTO 9 fluorescence emission was collected using a 530/30 nm emission (BL1), whereas propidium iodide fluorescence emission was collected using the 695/40 nm emission (BL2). In this example, a BL1 and SSC threshold was used, and results are shown without compensation. The live, SYTO 9–positive population is shown in green, and dead cells (Dead) are shown in red. Live and dead cell populations are easily distinguished.
Figure 2. Staining of E. coli cells using the BacLight LIVE/DEAD Bacterial Viability Kit using either 561 nm or 532 nm excitation of PI.E. coli cells were grown in lysogeny broth (LB) and harvested. Samples were analyzed on the Attune NxT Flow Cytometer at the 12.5 μL/min flow rate with an event rate of approximately 5,000 events/second using the blue 488 nm laser and 530/30 nm emission (BL1) for SYTO 9 detection, and (A) yellow 561 nm laser 620/15 nm emission (YL2) for detection of propidium iodide (PI). (B) Green 532 nm laser 620/15 nm emission (GL2) was used for detection of PI. BL1 and SSC thresholds were set and no compensation was performed. The live population of dividing cells (Live) is shown in green; dead cells (Dead) are shown in red. Excitation of SYTO 9 and PI with different lasers results in better separation of the populations.
Sample type | Demonstrated example | Published References |
---|---|---|
Algae | Nannochloropsis oculata | |
Bacteria | Escherichia coli | |
Bacillus subtilis | ||
Legionella strains | ||
Staphyloccus aureus | ||
Diatom | Phaeodactylum tricornutum | |
Human | Human adenocarcinoma | |
Human bone marrow (PBMCs) | ||
Human bone osteosarcoma cells (U2OS) | ||
Human cardiomyocytes | ||
Human corneal epithelial cells | ||
Human embryonic kidney (293FT) | ||
Human endothelial cells | ||
Human leukocytes | ||
Human mesenchymal stem cell (hMSC) | ||
Human red blood cells (RBC) | ||
Human T cell leukemia (Jurkat) | ||
Human whole blood | ||
Mouse | Mouse splenocytes | |
Mouse T and B regulatory cells | ||
Mouse cardiomyocytes | ||
Mouse bone marrow | ||
Mouse lung tissue | ||
Mouse tumor cells | ||
Mouse skeletal muscle | ||
Parasites | Plasmodium falciparum | |
L. amazonensis, T. brucei and T. cruzi | ||
Trypanosoma cruzi | ||
Picophytoplankton | Prochlorococcus spp; Synechococcus spp | |
Plants | Arabidopsis thaliana | |
Phalaris arundinacea | ||
Solidago canadensis | ||
Yeast | Candida albicans | |
Saccharomyces cerevisiae | ||
Zebra fish | Zebra fish organs |
Maintain your Attune NxT Acoustic Flow Cytometer in like-new condition by following these easy steps.
Increase your lab productivity and uptime, enabling you to focus on what matters most, your research success.
Product | AB Complete | AB Assurance | AB Maintenance |
---|---|---|---|
Standard on-site response time | Next business day* | 2 business days* | 3 business days** |
Scheduled on-site PM | |||
Remote instrument diagnostics | |||
Priority access to technical support (Monday–Friday, standard business hours) | |||
Parts, labor, and travel for repair | |||
Priority access to remote service engineer | |||
Post-PM requalification and critical repairs | |||
Field applications scientist (FAS) consultation |
* Availability limited in some geographic areas.
** After receipt of a purchase order.
Checks completed during planned maintenance visit | |
---|---|
System maintenance |
|
Computer maintenance |
|
Fluidics system maintenance |
|
Optics maintenance |
|
System diagnostics |
|
Attune NxT Autosampler maintenance (if applicable to unit) |
|
Instrument performance |
|
Get timely, cost-effective, and audit-ready documentation managed by a compliance specialist to help ensure your Attune NxT Flow Cytometer system is installed, operating, and performing to the manufacturer’s specifications.
Our factory-trained and experienced engineers will conduct Installation Qualification (IQ), Operational Qualification (OQ), and Instrument Performance Verification (IPV) on the Invitrogen Attune NxT Flow Cytometer system in your lab. Documentation for audits is provided.
Assay development & labeling services—our custom solutions to help develop the most appropriate assay for your research or discovery target of interest.
Custom antibodies—our custom antibody development, purification, and screening services deliver the quality, consistency, and performance you need.
Application and instrument training programs are available at our training centers located throughout the world, within your lab, or through web-based instruction. We offer a wide range of courses to build the skills of cellular analysis professionals and users of the Attune NxT Flow Cytometer:
Learn more about our Flow Cytometry Webinars
Take our T Cell Stimulation and Proliferation eLearning Course
Manage your instrument use and care online with Instrument Management. Whether you are responsible for one instrument or hundreds of instruments, Instrument Management enables you to easily manage your instrument use and care online.
Find out more about Instrument Management at thermofisher.com/easiertomanage
Designed for faster experimental run times, the Invitrogen Attune NxT compact flow cytometer offers rapid and accurate analysis for a broad range of sample types. Experience the difference.
The 40-page Flow Cytometer Evaluation Guide will enable a better understanding of the components and capabilities of various flow cytometers for objective comparison of instruments from several manufacturers.
Improve your workflow for fully automated sample acquisition from 96-well and 384-well microtiter plates. There are no physical changes to the system needed in order to run plate based experiments—everything is controlled in the software.
This grow-with-you system can be customized with up to 4 lasers and 14 colors and now up to 6 channels off of the violet laser. The optical system was designed to provide optimal signal and has the range to detect very bright as well as dim signals. The Attune NxT Flow Cytometer allows researchers the freedom to build multicolor panels, accommodating common fluorophores and dyes. Build your multicolor panel for the Attune NxT flow cytometer with our superior reagents and antibodies.
Lasers | Laser configuration Cat. No. | Lasers included, No. of detection channels | Total detection channels* | ||||
---|---|---|---|---|---|---|---|
Violet, 405 nm | Blue, 488 nm | Yellow, 561 nm | Green, 532 nm | Red, 637 nm | |||
1 | Blue A24864 A24864CFR** | Available as upgrade† |
4
| Available as upgrade† | Available as upgrade† | Available as upgrade† | 6 |
2 | Blue/green A28995 A28995CFR** | Available as upgrade† |
3
|
4
| Available as upgrade† | 9 | |
Blue/yellow A24861 A24861CFR** | Available as upgrade† |
3
|
4
| Available as upgrade† | 9 | ||
Blue/red A24863 A24863CFR** | Available as upgrade† |
4
| Available as upgrade† | Available as upgrade† |
3
| 9 | |
Blue/violet A24862 A24862CFR** |
4
|
4
| Available as upgrade† | Available as upgrade† | Available as upgrade† | 10 | |
Blue/violet 6 A29002 A29002CFR** |
6
|
3
| Available as upgrade† | Available as upgrade† | 11 | ||
3 | Blue/green/red A28997 A28997CFR** |
Available as upgrade†
|
3
|
4
|
3
| 12 | |
Blue/red/yellow A28993 A28993CFR** | Available as upgrade† | 3 | 4 | 3 | 12 | ||
Blue/green/violet A28999 A28999CFR** |
4
|
3
|
4
| Available as upgrade† | 13 | ||
Blue/violet/yellowA24859 A24859CFR** |
4
|
3
|
4
| Available as upgrade† | 13 | ||
Blue/red/violet A24860 A24860CFR** |
4
|
4
| Available as upgrade† | Available as upgrade† |
3
| 13 | |
Blue/red/violet 6 A29003 A29003CFR** |
6
|
3
| Available as upgrade† |
3
| 14 | ||
4 | Blue/red/violet/green A29001 A29001CFR** |
4
|
3
|
4
|
3
| 16 | |
Blue/red/yellow/violet A24858 A24858CFR** |
4
|
3
|
4
|
3
| 16 | ||
Blue/red/yellow/violet 6 A29004 A29004CFR** |
6
|
2
|
3
|
3
| 16 | ||
* Number of detection channels includes all fluorescence channels as well as a forward scatter and a side scatter channel. † Attune NxT Flow Cytometer owners wishing to purchase a laser upgrade should request a quote here: Yellow Laser Upgrade, Violet Laser Upgrade, Red Laser Upgrade, or Green Laser Upgrade. After we receive your quote request, a technical sales specialist will contact you about upgrade options. ** Instrument package comes with a 21 CFR part 11 Software License and an Attune NxT software license. |
A specification is derived from a specific test or calculation, but these tests aren’t standardized across instrument developers and may be misleading in a side-by-side comparison because their values may have been calculated in different ways, despite sharing the same terminology. This 8-page Comparing Technical Specification Documents resource offers helpful tips on deciphering variations.
Optics |
| ||||||||||||||||||||||||||||
Fluidics |
| ||||||||||||||||||||||||||||
Performance |
| ||||||||||||||||||||||||||||
Software |
| ||||||||||||||||||||||||||||
Quality and regulatory |
| ||||||||||||||||||||||||||||
Data management |
| ||||||||||||||||||||||||||||
Installation requirements |
|
Invitrogen Attune NxT Software is designed to provide powerful data acquisition and analysis using an intuitive, user-friendly interface. The instrument software features and benefits include:
Since its initial unveiling, the compact Attune NxT Flow Cytometer continues to offer more options and added functionality with additional lasers, more detection channel options, sample handling and robotic solutions.
Find laser upgrades, optional layout conversion kits, autosampler upgrades, robotics arm options, as well as filter kits and accessory parts.
Attune NxT upgrades | Filter | Common fluorochromes | Cat. No. |
---|---|---|---|
Attune NxT Yellow Laser Upgrade Kit | 585/16 | PE, Alexa Fluor 532, Alexa Fluor 568 | 100022779 |
620/15 | Alexa Fluor 594, PE-Texas Red, PE-Alexa Fluor 610 | ||
695/40 | PE-Alexa Fluor 700, TRI-COLOR (PE-Cy5), PE-Cy5.5, Qdot 705 | ||
780/60 | Qdot 800, PE-Cy 7, PE-Alexa Fluor 750 | ||
Attune NxT Red Laser Upgrade Kit | 670/14 | APC, Alexa Fluor 647, Qdot 655 | 100022778 |
720/30 | Alexa Fluor 680, Alexa Fluor 700, APC-Alexa Fluor 700, Qdot 705 | ||
780/60 | APC-Alexa Fluor 750, APC-Cy7, APC-eFluor 780, Qdot 800 | ||
Attune NxT Green Laser Upgrade Kit | 575/36 | PE, Alexa Fluor 532, Alexa Fluor 555, DsRed | A32701 |
620/15 | Alexa Fluor 568, Alexa Fluor 594, Qdot 605, PE-Texas Red, PE-Alexa Fluor 610 | ||
695/40 | Qdot 705, PE-Alexa Fluor 700, TRI-COLOR, PE-Cy5.5 | ||
780/60 | Qdot 800, PE-Cy7 | ||
Attune NxT Violet Laser Upgrade Kit | 440/50 | Super Bright 436, Alexa Fluor 405, eFluor 450, Pacific Blue | 100022777 |
512/25 | eFluor 506, Pacific Green | ||
603/48 | Super Bight 600, Pacific Orange, Qdot 605 | ||
710/50 | Super Bright 702, Qdot 705 | ||
Attune NxT Violet 6 Conversion Kit, Blue Laser | 530/30 | Alexa Fluor 488, FITC | A35428 |
574/26 | PE, PE-Alexa Fluor 610, Alexa Fluor 546 | ||
695/40 | PE-Alexa Fluor 700, PE-Cy5.5, PerCP, PerCP-Cy5.5, Qdot 705, TRI-COLOR | ||
Attune NxT Violet 6 Conversion Kit, Violet Laser | 450/40 | Super Bright 436, eFluor 450, Pacific Blue | A36569 |
525/50 | eFluor 506, Pacific Green, eFluor 506 | ||
610/20 | Super Bright 600, Qdot 605, Pacific Orange | ||
660/20 | Super Bright 645 | ||
710/50 | Super Bright 702, Qdot 705 | ||
780/60 | Super Bright 780 | ||
Attune NxT Violet 6 Conversion Kit, Red Laser | 670/14 | APC, Alexa Fluor 647, Qdot 655 | A36571 |
720/30 | Alexa Fluor 680, Alexa Fluor 700, APC-Alexa Fluor 700, Qdot 705 | ||
780/60 | APC-Alexa Fluor 750, APC-eFluor 780, APC-Cy7, Qdot 800 | ||
Attune NxT Violet 6 Conversion Kit, Yellow Laser | 585/16 | PE, Alexa Fluor 532, Alexa Fluor 568 | A36572 |
620/15 | Alexa Fluor 594, PE-Texas Red, PE-Alexa Fluor 610 | ||
695/40 | PE-Alexa Fluor 700, TRI-COLOR, PE-Cy5.5, Qdot 705 | ||
780/60 | Qdot 800, PE-Cy 7, PE-Alexa Fluor 750 |
NOTE: One accessory kit for each laser on your instrument needs to be purchased to successfully complete V6 instrument conversion.
The Invitrogen Attune NxT Flow Cytometer was developed with acoustic-assisted hydrodynamic focusing technology, and advanced fluidics designed to minimize clogging and effectively handle a broad range of cell types. This allows for a higher degree of data, detail, and throughput that enables processing of a large range of sample types, including large clumpy cells, samples with a low concentration of cells, and precious samples, more quickly and accurately than ever before with no loss in data quality.
Instantly search over 475 peer-reviewed scientific publications featuring the Invitrogen Attune NxT Flow Cytometer.
At Thermo Fisher Scientific, we are proud to receive the 2019 CiteAb award for the Attune NxT Flow Cytometer. CiteAb's Flow Cytometer to Watch in 2019 award was based on the number of published citations for the instrument.
The following applications were performed using the Attune NxT Flow Cytometer.
The Attune NxT Flow Cytometer enables well-separated bacterial populations. For researchers working with E. coli, the available green 532 nm laser delivers distinct separation of double-live populations of dividing cells (green) and dead (red) E. coli cells (Figures 1 and 2).
Figure 1. Staining of E. coli cells using the Invitrogen BacLight LIVE/DEAD Bacterial Viability Kit and blue laser excitation of Invitrogen SYTO 9 dye and propidium iodide (PI). Samples were analyzed on the Attune NxT Flow Cytometer at the 12.5 μL/min flow rate with an event rate of approximately 5,000 events/second. Instrument settings (voltages, threshold, and advanced settings) were set using single-color controls. The blue 488 nm laser was used for fluorescence excitation of both SYTO 9 dye and PI. SYTO 9 fluorescence emission was collected using a 530/30 nm emission (BL1), whereas propidium iodide fluorescence emission was collected using the 695/40 nm emission (BL2). In this example, a BL1 and SSC threshold was used, and results are shown without compensation. The live, SYTO 9–positive population is shown in green, and dead cells (Dead) are shown in red. Live and dead cell populations are easily distinguished.
Figure 2. Staining of E. coli cells using the BacLight LIVE/DEAD Bacterial Viability Kit using either 561 nm or 532 nm excitation of PI.E. coli cells were grown in lysogeny broth (LB) and harvested. Samples were analyzed on the Attune NxT Flow Cytometer at the 12.5 μL/min flow rate with an event rate of approximately 5,000 events/second using the blue 488 nm laser and 530/30 nm emission (BL1) for SYTO 9 detection, and (A) yellow 561 nm laser 620/15 nm emission (YL2) for detection of propidium iodide (PI). (B) Green 532 nm laser 620/15 nm emission (GL2) was used for detection of PI. BL1 and SSC thresholds were set and no compensation was performed. The live population of dividing cells (Live) is shown in green; dead cells (Dead) are shown in red. Excitation of SYTO 9 and PI with different lasers results in better separation of the populations.
Sample type | Demonstrated example | Published References |
---|---|---|
Algae | Nannochloropsis oculata | |
Bacteria | Escherichia coli | |
Bacillus subtilis | ||
Legionella strains | ||
Staphyloccus aureus | ||
Diatom | Phaeodactylum tricornutum | |
Human | Human adenocarcinoma | |
Human bone marrow (PBMCs) | ||
Human bone osteosarcoma cells (U2OS) | ||
Human cardiomyocytes | ||
Human corneal epithelial cells | ||
Human embryonic kidney (293FT) | ||
Human endothelial cells | ||
Human leukocytes | ||
Human mesenchymal stem cell (hMSC) | ||
Human red blood cells (RBC) | ||
Human T cell leukemia (Jurkat) | ||
Human whole blood | ||
Mouse | Mouse splenocytes | |
Mouse T and B regulatory cells | ||
Mouse cardiomyocytes | ||
Mouse bone marrow | ||
Mouse lung tissue | ||
Mouse tumor cells | ||
Mouse skeletal muscle | ||
Parasites | Plasmodium falciparum | |
L. amazonensis, T. brucei and T. cruzi | ||
Trypanosoma cruzi | ||
Picophytoplankton | Prochlorococcus spp; Synechococcus spp | |
Plants | Arabidopsis thaliana | |
Phalaris arundinacea | ||
Solidago canadensis | ||
Yeast | Candida albicans | |
Saccharomyces cerevisiae | ||
Zebra fish | Zebra fish organs |
Maintain your Attune NxT Acoustic Flow Cytometer in like-new condition by following these easy steps.
Increase your lab productivity and uptime, enabling you to focus on what matters most, your research success.
Product | AB Complete | AB Assurance | AB Maintenance |
---|---|---|---|
Standard on-site response time | Next business day* | 2 business days* | 3 business days** |
Scheduled on-site PM | |||
Remote instrument diagnostics | |||
Priority access to technical support (Monday–Friday, standard business hours) | |||
Parts, labor, and travel for repair | |||
Priority access to remote service engineer | |||
Post-PM requalification and critical repairs | |||
Field applications scientist (FAS) consultation |
* Availability limited in some geographic areas.
** After receipt of a purchase order.
Checks completed during planned maintenance visit | |
---|---|
System maintenance |
|
Computer maintenance |
|
Fluidics system maintenance |
|
Optics maintenance |
|
System diagnostics |
|
Attune NxT Autosampler maintenance (if applicable to unit) |
|
Instrument performance |
|
Get timely, cost-effective, and audit-ready documentation managed by a compliance specialist to help ensure your Attune NxT Flow Cytometer system is installed, operating, and performing to the manufacturer’s specifications.
Our factory-trained and experienced engineers will conduct Installation Qualification (IQ), Operational Qualification (OQ), and Instrument Performance Verification (IPV) on the Invitrogen Attune NxT Flow Cytometer system in your lab. Documentation for audits is provided.
Assay development & labeling services—our custom solutions to help develop the most appropriate assay for your research or discovery target of interest.
Custom antibodies—our custom antibody development, purification, and screening services deliver the quality, consistency, and performance you need.
Application and instrument training programs are available at our training centers located throughout the world, within your lab, or through web-based instruction. We offer a wide range of courses to build the skills of cellular analysis professionals and users of the Attune NxT Flow Cytometer:
Learn more about our Flow Cytometry Webinars
Take our T Cell Stimulation and Proliferation eLearning Course
Manage your instrument use and care online with Instrument Management. Whether you are responsible for one instrument or hundreds of instruments, Instrument Management enables you to easily manage your instrument use and care online.
Find out more about Instrument Management at thermofisher.com/easiertomanage
A software tool for exploring the hardware, software, optics and fluidics of the Attune NxT Flow Cytometer. Hot spots throughout the tool feature data, researcher opinions, and technical specifications. Mouse operation enables rotation, zoom, and measurement. Available in 8 languages.
Optimize your instrument performance, maximize the availability of your critical systems, and streamline your laboratory workflows. Choose the level of support for your Attune NxT Flow Cytometer that best meets your laboratory's needs and budget:
In addition to accessories such as sample syringes, find the Invitrogen Attune solutions necessary to operate your Attune NxT Flow Cytometer including:
This online tool guides you through flow cytometry panel design, providing a simplified, customizable experience to fit your flow cytometry panel design needs.
Use these quick guides to find antibodies and other conjugates of a broad range of fluorescent dyes, fluorescent proteins, Qdot probes, and phycobiliproteins.
This online tool allows researchers to assess the spectral compatibility of dyes and probes in the course of designing experiments that utilize fluorescence detection techniques.
Flow Cytometry Support Center – Find tips, troubleshooting help, and resources for your flow cytometry workflow.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.