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Resources for the Original Attune Flow Cytometer
First-generation Attune Flow Cytometer support
Find technical resources for the original first-generation Invitrogen Attune Flow Cytometer, including manuals, reagent selection tables, software download information, and ordering information for consumables and accessories for your instrument.
Expand your research capabilities with the next-generation Attune Flow Cytometers
Attune Flow Cytometer Software Demo
Reagent selection guide for the original Attune Flow Cytometer
| Excitation laser | Default filter (nm) | Filter range (nm) | Fluorophores | Other fluorescent reagents |
|---|---|---|---|---|
| Violet 405 nm | 450/40 | 430–470 | Alexa Fluor 405 Pacific Blue | PO-PRO-1 DyeCycle Violet Fixable Violet Dead Cell Stain CellTrace Violet Calcein Violet SYTOX Blue FxCycle Violet Click-iT Pacific Blue |
| 522/31 | 507–537 | Qdot 525 Horizon V500 | Fixable Aqua Dead Cell Stain F2N12S (apoptotic) | |
| 603/48 | 579–627 | Pacific Orange Qdot 605 | Fixable Yellow Dead Cell Stain F2N12S (live) | |
| Blue 488 nm | 530/30 | 515–545 | Alexa Fluor 488 Fluorescein | Calcein Fluo-3/Fluo-4 TO-PRO-1 iodide CFSE GFP JC-1/DiOC2(3) SYTOX Green DyeCycle Green Rhodamine 123 YO-PRO-1 iodide Fixable Green Dead Cell Stain Click-iT Alexa Fluor 488 |
| 574/26 | 561–587 | PE | PI Fura Red AM cell permeant DyeCycle Orange JC-1/DiOC2(3) pHrodo Phagocytosis Particle Labeling Kit SNARF (low pH) isomers SYTOX Orange Dead cell stain | |
| 640LP | >640 | PE-Alexa Fluor 610 PerCP PE-Alexa Fluor 700 PE-Cy5.5 TRI-COLOR (TC) PE-Cy5 PerCP-Cy5.5 PE-Cy7 PE-PE-Alexa Fluor 750 Qdot 655 Qdot 705 Qdot 800 | JC-1/DiOC2(3) Fixable Red Dead Cell Stain 7-AAD SNARF (high pH) isomers SYTOX AADvanced Dead Cell Stain DyeCycle Ruby |
| Excitation laser | Default filter (nm) | Filter range (nm) | Fluorophores | Other fluorescent reagents |
|---|---|---|---|---|
| Blue 488 nm | 530/30 | 515–545 | Alexa Fluor 488 Fluorescein | Calcein Fluo-3/Fluo-4 TO-PRO-1 iodide CFSE GFP JC-1/DiOC2(3) SYTOX Green DyeCycle Green Rhodamine 123 YO-PRO-1 iodide Fixable Green Dead Cell Stain Click-iT Alexa Fluor 488 |
| 574/26 | 561–587 | PE | PI Fura Red AM cell permeant DyeCycle Orange JC-1/DiOC2(3) pHrodo Phagocytosis Particle Labeling Kit SNARF (low pH) isomers SYTOX Orange Dead cell stain | |
| 690/50 | 665–715 | Qdot 705 PerCP TRI-COLOR PE-Cy5 PerCP-Cy5.5 PE-Cy5.5 | Vybrant Dye Cycle Ruby | |
| 780/60 | 750–810 | PE-Alexa Fluor 750 Qdot 800 PE-Cy 7 | Vybrant Dye Cycle Ruby | |
| Red 637 nm | 660/20 | 650–670 | Allophycocyanin (APC) Alexa Fluor 647 | Fixable Far Red Dead Cell Stain Click-iT Alexa Fluor 647 FxCycle Far Red SYTOX Red Dead cell stain TO-PRO-3 |
| 780/60 | 750–810 | APC-Alexa Fluor 750 APC-Cy 7 | Fixable Near-IR Dead Cell Stain Vybrant Dye Cycle Ruby |
The Invitrogen Attune Autosampler is compatible with the original Attune Flow Cytometer and gives you the flexibility of automated multi-sample processing, offering:
- Broad compatibility—compatible with many different plate formats, including 96-well, 384-well, and deep-well plates.
- Mixing by aspiration—mixing sample by aspiration instead of shaking ensures homogeneity of the sample and helps maintains cell viability
- Intelligent probe—intelligent probe design minimizes clogging and carryover (<0.5%) and prevents damage to the instrument
- Auto cleaning—performs automated cleaning when the instrument is shutting down
- Consistent data—Designed to provide minimal variation regardless of sampling method (tube vs. plate) and collection rate
See how the CytKick and CytKick Max autosamplers work with the Attune CytPix and Attune NxT flow cytometers by requesting an in-lab demo from one of our trained representatives.
Attune cytometric software
The software for the original Attune Flow Cytometer can be downloaded without licensing fees and can be added to any desktop or laptop computer at your institution without any additional costs. Results can be easily analyzed at your convenience at your own computer, allowing the next user to run experiments on the Attune cytometer. Before initiating the download process, use the table below to determine which software version your system uses.
| Attune Cytometer WITHOUT Autosampler | Attune Cytometer WITH Autosampler | ||
|---|---|---|---|
 |  | ||
| Purchase date of Attune Cytometer | BEFORE January 1st, 2013 | AFTER January 1st, 2013 | — |
| Software version | v1.2.5 * | v2.1.0 † | v2.1.0 † |
| Operating system | Windows™ XP | 64-bit Windows 7 | 64-bit Windows 7 |
| * To get the benefits of the v2.1.0 software requires the purchase of an Upgrade Kit; or you can upgrade to v2.1.0 for free with the purchase of an Attune Autosampler. † Version 2.1.0 requires a 64-bit Windows 7 computer. 8 GB RAM with minimal 500 GB storage are recommended. | |||
Why should I upgrade to v2.1.0?
If you purchased your Attune Cytometer without an Autosampler before January 1, 2013, you may be running software v1.2.5 on a Windows XP system. To get the benefits of the v2.1.0 software requires the purchase of an upgrade kit; or you can upgrade to v2.1.0 for free with the purchase of an Attune Autosampler.
- Better performance—responsiveness of software v2.1.0 is dramatically improved over v1.2.5
- Faster analysis of samples—save time with batch processing, ability to select multiple objects, and easier experiment setup and data analysis
- Sample recovery—no other syringe-driven system offers the ability to recover unused samples
- Increased functionality—additional functionality beyond supporting the use of 96- and 384-well plates
The Attune Computer Upgrade Kit (Cat. No. 4481653) includes:
- A Dell™ 64-bit computer with the Windows 7 operating system
- Attune Cytometric Software v2.1 and v2.1 Documentation Kit
- A visit by trained service personnel to assist in the transfer of data from the existing computer system
Download the Upgrade Kit Flyer
There are two ways to purchase the upgrade kit:
- Standalone kit: Attune Software (v2.1.0) and Computer Upgrade Kit, including on-site installation and data transfer (Cat. No. 4481653); or
- Kit + Service Contract: 1-year service contract and the upgrade kit together (35% savings compared to purchasing separately). Contact your sales rep to request a quote.