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MicroRNAs (miRNAs) are small, noncoding RNA molecules that direct posttranscriptional suppression of gene expression. They have been shown to act as key regulators in many basic biological processes such as development, cell proliferation, differentiation, and the cell cycle. Emerging evidence also implicates miRNAs in the pathogenesis of human diseases such as cancers, metabolic diseases, neurological disorders and infectious diseases.
Applied Biosystems TaqMan MicroRNA Assays are innovative tools for miRNA research-from isolation through discovery, profiling, quantitation, validation and functional analysis. We have developed two methods for sensitive, precise quantification and profiling of miRNA using real-time PCR TaqMan probe-based analysis.
Isolate RNA using methods that preserve RNA integrity and expression profiles
Not required for Applied Biosystems TaqMan Assays. These assays have passed our industry leading bioinformatics design criteria.
Convert your RNA into cDNA, over a wide range of RNA concentrations.
TaqMan Advanced miRNA cDNA Synthesis Kit
Provides miR-Amp Universal Amplification with universal primers and master mix to increase the amount of cDNA for each target with accurate detection as few as 60 copies from as little as 1pg of total RNA or 2 μL of serum/plasma. Pre-Amplification Step for TaqMan Advanced miRNA Assays.
Note that a pre-amplification step is performed after the RT. The miR-Amp master mix and miR-Amp primer mix are included in the TaqMan Advanced miRNA cDNA Synthesis Kit.
TaqMan MicroRNA Reverse Transcription Kit
Classic TaqMan microRNA kit for use with the TaqMan MicroRNA Assays to convert miRNA to cDNA (miRNA specific RT). Best for 1-10 targets. Not compatible with TaqMan Advanced miRNA products.
Megaplex RT Primers (For TAC and OpenArray)
Designed to streamline experimental workflows, Megaplex RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis on a TaqMan MicroRNA Array.
Megaplex PreAmp Primers
Pre-amplification and reverse transcription primer for use with the classic TaqMan MicroRNA assay (not for use with TaqMan Advanced MicroRNA products) when sensitivity is of the utmost importance, Megaplex PreAmp Primers significantly enhance the detection of miRNAs expressed at low levels, enabling the generation of a comprehensive expression profile using very little sample—as little as 1 ng of input total RNA.
Note: When used with - OpenArray, TAC with less than 350ng of RNA and optional for TaqMan Array Cards with greater than 350ng of RNA. For single tube assays you can run pre-amplification with the appropriate protocol.
TaqMan PreAmp Master Mix
Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan Gene Expression assays
Instruments for real time PCR and digital PCR
Process and interpret qPCR data with intuitive software data analysis tools for a range of applications
Isolate RNA using methods that preserve RNA integrity and expression profiles
Not required for Applied Biosystems TaqMan Assays. These assays have passed our industry leading bioinformatics design criteria.
Convert your RNA into cDNA, over a wide range of RNA concentrations.
TaqMan Advanced miRNA cDNA Synthesis Kit
Provides miR-Amp Universal Amplification with universal primers and master mix to increase the amount of cDNA for each target with accurate detection as few as 60 copies from as little as 1pg of total RNA or 2 μL of serum/plasma. Pre-Amplification Step for TaqMan Advanced miRNA Assays.
Note that a pre-amplification step is performed after the RT. The miR-Amp master mix and miR-Amp primer mix are included in the TaqMan Advanced miRNA cDNA Synthesis Kit.
TaqMan MicroRNA Reverse Transcription Kit
Classic TaqMan microRNA kit for use with the TaqMan MicroRNA Assays to convert miRNA to cDNA (miRNA specific RT). Best for 1-10 targets. Not compatible with TaqMan Advanced miRNA products.
Megaplex RT Primers (For TAC and OpenArray)
Designed to streamline experimental workflows, Megaplex RT Primers provide a single reaction solution when preparing cDNA for real-time PCR analysis on a TaqMan MicroRNA Array.
Megaplex PreAmp Primers
Pre-amplification and reverse transcription primer for use with the classic TaqMan MicroRNA assay (not for use with TaqMan Advanced MicroRNA products) when sensitivity is of the utmost importance, Megaplex PreAmp Primers significantly enhance the detection of miRNAs expressed at low levels, enabling the generation of a comprehensive expression profile using very little sample—as little as 1 ng of input total RNA.
Note: When used with - OpenArray, TAC with less than 350ng of RNA and optional for TaqMan Array Cards with greater than 350ng of RNA. For single tube assays you can run pre-amplification with the appropriate protocol.
TaqMan PreAmp Master Mix
Stretch as little as 1 ng of cDNA into 200 real-time PCR reactions for gene expression analysis using TaqMan Gene Expression assays
Instruments for real time PCR and digital PCR
Process and interpret qPCR data with intuitive software data analysis tools for a range of applications
We have more options for performing microRNA and ncRNA studies using real-time PCR.
For Research Use Only. Not for use in diagnostic procedures.