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Mixed-mode chromatography is a versatile separation technique that utilizes multiple modes of separation to achieve greater selectivity and specificity in separating complex mixtures of biological molecules. It combines the principles of both ion exchange and hydrophobic interactions to achieve efficient separation and purification of complex mixtures.
Unlike traditional chromatography methods that solely rely on a single interaction mechanism, mixed-mode chromatography employs a unique stationary phase that contains both hydrophobic and charged functional groups. This allows for a broader range of interactions with the target molecules, resulting in enhanced selectivity and increased resolution. By utilizing this combined approach, mixed-mode chromatography offers a powerful tool for scientists to separate and purify diverse compounds, making it an invaluable tool in the field of biotechnology for drug development and protein purification.
Thermo Scientific POROS Caprylate Mixed-Mode Cation Exchange Chromatography Resin is designed to remove moderate to high levels of aggregates during downstream processing of therapeutic antibodies in flow-through mode. As a mixed-mode, weak cation-exchange resin, POROS Caprylate effectively removes other impurities, such as host cell proteins and a wide range of high molecular weight species.
The unique resin backbone consists of crosslinked poly(styrene-divinylbenzene) with a superior pore structure that provides rapid mass transport and facilitates increased productivity. The particle surface is coated with a proprietary hydrophilic polymer, which is further derivatized with caprylic acid.
Learn more about wider operational space, which allows for effective removal of aggregates and challenging molecules with high levels of aggregates, in this scientific poster.
Check out the new Chromatography Learning Lab from Thermo Fisher Scientific, providing a robust range of webinars, articles, eBooks, infographics, and other digital resources to help you optimize the use of our purification products in your process.
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Pharmaceutical Grade Reagent. For Manufacturing and Laboratory Use Only.