Site-Directed Mutagenesis

Robust, high-efficiency systems and kits to streamline your workflow

• Speed—time-to-results is typically less than 3 hours for 3 kb plasmid
• Precision—alter up to 25 nucleotides (nt) when only one site is mutated
• Flexibility—generate substitutions, deletions, or insertions in virtually any plasmid
• Simplicity—free online tool guides you through construct and primer design and generates final construct sequence for easy implementation

High mutagenesis efficiency. Multiple base mutagenesis is common, and we tested a 12 base substitution, insertion and deletion using a pUC19 plasmid. A random 12 base substitution was carried out within a single mutated primer. Alternatively, a random 12 base oligonucleotide containing a stop codon was inserted into wild type pUC19 plasmid, followed by deletion of the exact 12 bases to restore the wild type plasmid.

High mutagenesis efficiency. The mutagenesis efficiency for a 12 base substitution, insertion or deletion was above 90%. The performance of GeneArt Site-Directed Mutagenesis kit was comparable to the latest generation of kits from competitor 'Q'.

Multisite-directed mutagenesis efficiency. Figure indicates the multisite-directed mutagenesis efficiency of 3 sites of 1 bp or 3 bp each in 5, 10, and 14 kb plasmids. In all cases the mutated sites (1 or 3 bp each) included one insertion, one deletion and one substitution. The performance of GeneArt Site-Directed Mutagenesis PLUS System was comparable to the latest generation of multisite-directed mutagenesis kits from the competitor.