Primer Designer Tool for PCR & Sanger Sequencing

Streamline your workflow with our primer design tool

From design to synthesis, quality primers are vital to successful results. Use our online Applied Biosystems™ Primer Designer™ Tool to search for the right PCR/Sanger sequencing primer pair from a database of ~650,000 predesigned primer pairs for resequencing the human exome and human mitochondrial genome. Choose from different amplicon lengths to accommodate various research applications and biological sample types.

• Our primers are free of known SNPs and primer-dimers, highly target-specific and used under universal PCR conditions 
• Full primer coverage for Ion Torrent™ AmpliSeq™ Exome Panel and Ion Torrent™ AmpliSeq™ Cancer Hotspot Panel v.2 Sanger confirmation workflow
• Flexible primer configuration to meet your research needs: primers can be ordered unmodified, M13-tailed, HPLC-purified or desalted
• All the primers have been checked by mass spectrometry and have passed stringent bioinformatics metrics; lab bench validation tests have shown >95% success rate

How to Use the Primer Designer™ Tool

No time for primer design?

From design to synthesis, quality primers are vital to successful results. Search our tool, to find and order pre-designed primers for PCR and Sanger sequencing. Free of SNPs and primer-dimers, highly target-specific, and used under universal PCR conditions - these primers can be ordered unmodified, M13-tailed, HPLC-purified, or desalted. All are checked by mass spectometry. Have confidence knowing primer pairs are selected to tile across exons for optimal exon coverage with a minimal number of primer pairs.

Save time with the Primer Designer™ Tool by selecting from >300,000 primer pairs targeting the human exome.

(File must be less than 500 kb in size and contain less than 1000 unique line-items.)

For Research Use Only. Not for use in diagnostic procedures.

*This promotion is open to customers in Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Hungary, Italy, The Netherlands, Norway, Poland, Portugal, Slovakia, South Africa, Spain, Sweden, Switzerland and the United Kingdom only. Discount will apply to qualifying orders received no later than December 31, 2016. Customer can use the discount only once. Discount applies to local list price in effect at the time the order is received. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.