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BioTek’s Synergy™ H4 and Synergy™ 2 multi-mode microplate readers have received the LanthaScreen® Certified Plus designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance.
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Before Using LanthaScreen® Terbium and Europium Assays
Test Your Plate Reader Set-up
These technical notes provide a method for verifying that a fluorescent plate reader is able to detect a change in time-resolved fluorescence energy transfer (TR-FRET) signal, confirming proper instrument set-up and a suitable response. The method is independent of any biological reaction or equilibrium and uses reagents that are on-hand for the LanthaScreen® assay.
Synergy™ H4 Hybrid Multi-Mode Microplate Reader
Instrument Configuration:
| Assay Technology | Adapta® & LanthaScreen® Europium | GeneBLAzer® | LanthaScreen® Terbium | Predictor™ | Z'-LYTE® |
|---|---|---|---|---|---|
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FP | FRET |
| Setup Guide |
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| Application Note | View | View | View | View | View |
| Poster | - | View | View | View | View |
| Excitation Filter (18 mm) | 340/30 | 400/30 | 340/30 | 530/25 | 400/30 |
| Emission 1 Filter (18 mm) | 620/10 | 460/40 | 495/10 | 590/35 | 460/40 |
| Emission 2 Filter (18 mm) | 665/8 | 528/20 | 520/25 | 590/35 | 528/20 |
| Dichroic Mirror | 400 | NA | 400 | 570 | 435 |
Synergy™ 2 Multi-Mode Microplate Reader
- Learn more about the Synergy™ 2 Multi-Mode Microplate Reader
Instrument Configuration:
| Assay Technology | Adapta® & LanthaScreen® Europium | GeneBLAzer® | LanthaScreen® Terbium | Predictor™ | Z'-LYTE® |
|---|---|---|---|---|---|
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FP | FRET |
| Setup Guide |
|
|
|
|
|
| Application Note | View | View | View | View | View |
| Poster | - | View | View | View | View |
| Excitation Filter (18 mm) | 340/30 | 400/30 | 340/30 | 530/25 | 400/30 |
| Emission 1 Filter (18 mm) | 620/10 | 460/40 | 495/10 | 590/35 | 460/40 |
| Emission 2 Filter (18 mm) | 665/8 | 528/20 | 520/25 | 590/35 | 528/20 |
| Dichroic Mirror | 400 | NA | 400 | 570 | 435 |
Synergy™ HT Multi-Mode Microplate Reader
Instrument Configuration:
| Assay Technology | Adapta® & LanthaScreen® Europium | GeneBLAzer® | LanthaScreen® Terbium | Predictor™ | Z'-LYTE® |
|---|---|---|---|---|---|
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FP | FRET |
| Setup Guide | Reader not compatible | Reader not tested | Reader not compatible | Reader not compatible | Reader not tested |
| Application Note | - | View | - | - | View |
| Poster | - | - | - | - | - |
| Excitation Filter (18 mm) | - | 400/30 | - | - | 400/30 |
| Emission 1 Filter (18 mm) | - | 460/40 | - | - | 460/40 |
| Emission 2 Filter (18 mm) | - | 528/20 | - | - | 528/20 |
FLx800™ Fluorescence Microplate Reader
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Instrument Configuration:
| Assay Technology | Adapta® & LanthaScreen® Europium | GeneBLAzer® | LanthaScreen® Terbium | Predictor™ | Z'-LYTE® |
|---|---|---|---|---|---|
| Modality | TR-FRET | FRET (bottom) | TR-FRET | FP | FRET |
| Setup Guide | Reader not compatible |
| Reader not compatible | Reader not compatible |
|
| Application Note | - | - | - | - | - |
| Poster | - | View | - | - | View |
| Excitation Filter (18 mm) | - | 400/30 | - | - | 400/30 |
| Emission 1 Filter (18 mm) | - | 460/40 | - | - | 460/40 |
| Emission 2 Filter (18 mm) | - | 528/20 | - | - | 528/20 |
* Filters (diameter) shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm). Contact BioTek Instruments for information on ordering filters and dichroic mirrors suitable with the microplate readers listed above.
Synergy™ and FLx800™ are trademarks of BioTek Instruments.
For Research Use Only. Not for use in diagnostic procedures.