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NovaFluor Yellow 730 Dye
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| 3 | 561 | 695/40 | 551 | 730 | (in buffer) 5 | flow cytometry |
Invitrogen NovaFluor Yellow 730 dye can be used as an alternative to PE-Cyanine 5.5, with far less cross-laser excitation and spectral spillover. NovaFluor Yellow 690, NovaFluor Yellow 700 and NovaFluor Yellow 730 dyes cannot be used together with conventional flow cytometry, but their distinct spectral signature allows them to be successfully unmixed using a spectral flow cytometer.
The macromolecule-based NovaFluor Yellow 730 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
- Narrow emission spectrum and minimal cross-laser excitation for better resolution
- Bright fluorescence allows it to be paired with medium-to-low abundance antigens
- Can serve as a replacement for PE-Cy5.5, with far less cross-laser excitation and spectral spillover
- Can be used together with NovaFluor Yellow 690 and NovaFluor Yellow 700 dyes using a spectral flow cytometer
- Produces highly stable fluorescence and is compatible with all buffers tested
Click image to enlarge
Spectral signature of NovaFluor Yellow 730 dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Yellow 730 were used for analysis. Data were acquired on a 5-laser Cytek Aurora system.
Staining of human peripheral blood cells with NovaFluor Yellow 730 dye conjugated antibody. Normal human peripheral blood cells (PBMCs) were unstained (left) or stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Yellow 730 (right). Data were acquired in the YG7 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.