NovaBright™ Chemiluminescent Beta-galactosidase Reporter Gene Assays

NovaBright beta-galactosidase reporter gene assay for mammalian or yeast cells combines direct cell lysis with the rapid ultrasensitive detection of beta-galactosidase reporter enzyme in a homogenous assay format. The kit uses the combination of Galacton-Star substrate and Sapphire-II enhancer to give sensitivity and dynamic range unmatched by colorimetric methods. The kit is formatted with two alternative reaction buffers for the lysis of mammalian or yeast cells.

NovaBright β-galactosidase Reporter Gene Assays

NovaBright β-galactosidase Reporter Gene Assays are available for yeast or mammalian cells and combine direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase.

  • Homogeneous assay is automation-friendly
  • Persistent glow readout remains constant for 45 to 90 minutes
  • Sensitivity from picogram to nanogram amounts of reporter
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Sensitivity of NovaBright β-galactosidase Reporter Gene Assay Detection. NovaBright β-galactosidase Reporter Gene Assays were performed with the indicated amount of purified β-galactosidase diluted in PBS. Assays were measured on a microplate luminometer.

Workflow

NovaBright™ Beta-gal Report Gene Assay Workflow

More NovaBright Chemiluminescent Reporter Gene Assays

Resources

For Research Use Only. Not for use in diagnostic procedures.