Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Nucleotide | MW (g/mol) |
---|---|
Ribonucleotide Triphosphates (Avg. MW = 499.5) | |
ATP | 507.2 |
CTP | 483.2 |
GTP | 523.2 |
UTP | 484.2 |
Ribonucleotide Monophosphates (Avg. MW = 339.5) | |
AMP | 347.2 |
CMP | 323.2 |
GMP | 363.2 |
UMP | 324.2 |
Deoxyribonucleotide triphosphates (Avg. MW = 487.0) | |
dATP | 491.2 |
dCTP | 467.2 |
dGTP | 507.2 |
dTTP | 482.2 |
Deoxyribonucleotide Monophosphates (Avg. MW = 327.0) | |
dAMP | 331.2 |
dCMP | 307.2 |
dGMP | 347.2 |
dTMP | 322.2 |
Exact M.W. of ssRNA (e.g., RNA Transcript):
M.W. = (An x 329.2) + (Un x 306.2) + (Cn x 305.2) + (Gn x 345.2) + 159ª
An, Un, Cn, and Gn are the number of each respective nucleotide within the polynucleotide.
M.W. calculated is valid at physiological pH.
ªAddition of "159" to the M.W. takes into account the M.W. of a 5' triphosphate.
Exact M.W. of ssDNA (e.g., Oligonucleotides):
M.W. = (An x 313.2) + (Tn x 304.2) + (Cn x 289.2) + (Gn x 329.2) + 79.0ª
An, Tn, Cn, and Gn are the number of each respective nucleotide within the polynucleotide.
ªAddition of "79.0" to the M.W. takes into account the 5' monophosphate left by most restriction enzymes. No phosphate is present at the 5' end of strands made by primer extension.
Approximate M.W.'s of Nucleic Acids:
M.W. of ssRNA = (# nucleotides x 320.5) + 159.0
M.W. of ssDNA = (# nucleotides x 303.7) + 79.0
M.W. of dsDNA = (# nucleotides x 607.4) + 157.9
Size (nt) | Daltons or g/mol | 1 µg equivalent | |
---|---|---|---|
pmol | molecules | ||
20 | 6,569 | 152.23 | 9.17 x 1013 |
100 | 32,209 | 31.05 | 1.87 x 1013 |
300 | 96,309 | 10.38 | 6.25 x 1012 |
500 | 160,409 | 6.23 | 3.75 x 1012 |
1000 | 320,659 | 3.12 | 1.88 x 1012 |
1800 | 577,059 | 1.73 | 1.04 x 1012 |
Size (nt) | Daltons or g/mol | 1 µg equivalent | |
---|---|---|---|
pmol | molecules | ||
20 | 6,153 | 162.52 | 9.79 x 1013 |
100 | 30,449 | 32.84 | 1.98 x 1013 |
300 | 91,189 | 10.97 | 6.6 x 1012 |
500 | 151,929 | 6.58 | 3.96 x 1012 |
1000 | 303,779 | 3.29 | 1.98 x 1012 |
1800 | 546,739 | 1.83 | 1.1 x 1012 |
Size (nt) | Daltons or g/mol | 1 µg equivalent | |
---|---|---|---|
pmol | molecules | ||
20 | 12,306 | 81.26 | 4.89 x 1013 |
100 | 60,898 | 16.42 | 9.89 x 1012 |
300 | 182,378 | 5.48 | 3.3 x 1012 |
500 | 303,858 | 3.29 | 1.98 x 1012 |
1000 | 607,558 | 1.65 | 9.91 x 1011 |
1800 | 1,093,478 | 0.91 | 5.51 x 1011 |
1 A260 dsDNA = 50 µg/ml
1 A260 ssDNA = 37 µg/ml
1 A260 ssRNA = 40 µg/ml
Nucleotide | Absorbance Maximum at pH 7.0 (nm) | Molar Extinction Coefficient at pH 7.0 |
---|---|---|
ATP | 259 | 15,400 |
CTP | 271 | 9,000 |
GTP | 253 | 13,700 |
UTP | 262 | 10,000 |
dATP | 259 | 15,200 |
dCTP | 271 | 9,300 |
dGTP | 253 | 13,700 |
dTTP | 267 | 9,600 |