RNA Silica Spin Column with RNA around the column

PureLink Purification Solutions

The Invitrogen PureLink nucleic acid purification kits are a family of purification products designed to meet the challenges posed by specific nucleic acid types, sample sources and volumes, throughput levels and downstream applications. These kits are based on either silica-membrane, anion exchange resin, or advanced silica-membrane purification technologies.

Learn more from our application notes:

PureLink Spin Column Kits

The PureLink spin column kits are a family of fast, simple, and cost-effective nucleic acid purification kits designed for the preparation of genomic DNA, plasmid DNA, total RNA, microRNAs and formalin-fixed paraffin-embedded (FFPE) RNA. These kits make use of proven silica membrane technology and eliminate the complex steps associated with older methodologies. Kits are available for both low-throughput (single samples) and high-throughput (96- and 384-sample) applications.

Obtain more RNA from your samples. RNA mini kits have 6 to 10 times the input capacity and higher yields compared to other suppliers’ kits.

Nucleic AcidApplicationProduct
RNAViral RNA or DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatantsPureLink Viral RNA/DNA Mini Kit
Total RNA from animal and plant cells, tissues, whole blood, yeast, bacteriaPureLink RNA Mini Kit
Whole BloodPureLink Total RNA Blood Kit
Total RNA from bacteria, yeast, plants and mammalian cells—96 wellPureLink 96 RNA Purification System
MicroRNA (miRNA)PureLink miRNA Isolation Kit
Total RNA from formalin-fixed paraffin-embedded (FFPE) samplesPureLink FFPE RNA Isolation Kit
Graph showing graph with different amounts of brain tissue input for RNA isolation comparing two suppliers

Figure 1. RNA yield from mouse brain tissue as determined by RNA analysis on a Qubit. Input volume of 150 mg for Qiagen not performed because it is above the 30 mg input capacity of the kit.

PureLink plasmid DNA kits are available is a variety of formats to best fit your downstream experiments. These range from mini and maxi kits for prokaryotic systems to low endotoxin kits for transfection into mammalian cells. Figure 2 illustrates how you can achieve high yields of endotoxin-free, advanced transfection–quality plasmid DNA with PureLink Expi as compared to kits from a competitor.

Nucleic AcidApplicationProduct
Plasmid DNAManual miniprep samples—Molecular Biology gradePureLink Quick Plasmid Miniprep Kit
Manual miniprep samples—Transfection gradePureLink HQ Mini Plasmid Purification Kits
and PureLink HQ 96 Mini Plasmid Purification Kits
High-throughput plasmid minipreps—96-well  Transfection gradePureLink HQ Mini Plasmid Purification Kits
and PureLink HQ 96 Mini Plasmid Purification Kits
Megaprep and Gigaprep—Transfection gradePureLink HiPure Expi Plasmid Megaprep and Gigaprep Kits
Sensitive cell lines; transfection of standard, robust cell lines; all molecular biology applicationsPureLink Fast Low Endotoxin Plasmid Midi and Maxi
3 graphs of midi, mega, and maxi plasmid preps showing yield vs supplier

Figure 2. Two high-copy plasmids with different backbones were purified using PureLink Expi and Qiagen EndoFree maxiprep, megaprep, and gigaprep kits as described in the product manuals. Data for DNA yields are shown in the graph; endotoxin values (EU/μg) are provided only for PureLink Expi preps.

PureLink genomic DNA (gDNA) kits isolate highly purified gDNA from a variety of samples including mammalian cells, bacteria, blood, and tissues. Figure 3 compares the results of gDNA isolated from cultured cells isolated using different suppliers.

Nucleic AcidApplicationProduct
Genomic DNAAnimal tissues and cellsPureLink Genomic DNA
Plant tissuesPureLink Genomic Plant DNA Purification Kit
DNAAgarose gel extractionPureLink Gel Extraction Kit
PCR reaction clean-upPureLink PCR Purification Kit
Graph showing higher yields achieved with the PureLink Genomic DNA Mini Kit compared to those obtained with another supplier’s kit

Figure 3. Higher yields achieved with the PureLink Genomic DNA Mini Kit compared to those obtained with another supplier’s kit.

RNA isolation

Obtain more RNA from your samples. RNA mini kits have 6 to 10 times the input capacity and higher yields compared to other suppliers’ kits.

Nucleic AcidApplicationProduct
RNAViral RNA or DNA from fresh or frozen cell-free biological fluids (plasma, serum, cerebrospinal fluid) and cell culture supernatantsPureLink Viral RNA/DNA Mini Kit
Total RNA from animal and plant cells, tissues, whole blood, yeast, bacteriaPureLink RNA Mini Kit
Whole BloodPureLink Total RNA Blood Kit
Total RNA from bacteria, yeast, plants and mammalian cells—96 wellPureLink 96 RNA Purification System
MicroRNA (miRNA)PureLink miRNA Isolation Kit
Total RNA from formalin-fixed paraffin-embedded (FFPE) samplesPureLink FFPE RNA Isolation Kit
Graph showing graph with different amounts of brain tissue input for RNA isolation comparing two suppliers

Figure 1. RNA yield from mouse brain tissue as determined by RNA analysis on a Qubit. Input volume of 150 mg for Qiagen not performed because it is above the 30 mg input capacity of the kit.

Plasmid DNA isolation

PureLink plasmid DNA kits are available is a variety of formats to best fit your downstream experiments. These range from mini and maxi kits for prokaryotic systems to low endotoxin kits for transfection into mammalian cells. Figure 2 illustrates how you can achieve high yields of endotoxin-free, advanced transfection–quality plasmid DNA with PureLink Expi as compared to kits from a competitor.

Nucleic AcidApplicationProduct
Plasmid DNAManual miniprep samples—Molecular Biology gradePureLink Quick Plasmid Miniprep Kit
Manual miniprep samples—Transfection gradePureLink HQ Mini Plasmid Purification Kits
and PureLink HQ 96 Mini Plasmid Purification Kits
High-throughput plasmid minipreps—96-well  Transfection gradePureLink HQ Mini Plasmid Purification Kits
and PureLink HQ 96 Mini Plasmid Purification Kits
Megaprep and Gigaprep—Transfection gradePureLink HiPure Expi Plasmid Megaprep and Gigaprep Kits
Sensitive cell lines; transfection of standard, robust cell lines; all molecular biology applicationsPureLink Fast Low Endotoxin Plasmid Midi and Maxi
3 graphs of midi, mega, and maxi plasmid preps showing yield vs supplier

Figure 2. Two high-copy plasmids with different backbones were purified using PureLink Expi and Qiagen EndoFree maxiprep, megaprep, and gigaprep kits as described in the product manuals. Data for DNA yields are shown in the graph; endotoxin values (EU/μg) are provided only for PureLink Expi preps.

Genomic DNA isolation

PureLink genomic DNA (gDNA) kits isolate highly purified gDNA from a variety of samples including mammalian cells, bacteria, blood, and tissues. Figure 3 compares the results of gDNA isolated from cultured cells isolated using different suppliers.

Nucleic AcidApplicationProduct
Genomic DNAAnimal tissues and cellsPureLink Genomic DNA
Plant tissuesPureLink Genomic Plant DNA Purification Kit
DNAAgarose gel extractionPureLink Gel Extraction Kit
PCR reaction clean-upPureLink PCR Purification Kit
Graph showing higher yields achieved with the PureLink Genomic DNA Mini Kit compared to those obtained with another supplier’s kit

Figure 3. Higher yields achieved with the PureLink Genomic DNA Mini Kit compared to those obtained with another supplier’s kit.

For additional information on our Plasmid Purification products go to Plasmid DNA Isolation

Select peer-reviewed publications

  1. Sathiamoorthy, S., Malott, R. J., Gisonni-Lex, L., et al. (2018). Selection and evaluation of an efficient method for the recovery of viral nucleic acid extraction from complex biologicals. NPJ vaccines, 3(1), 31.
  2. Bunker, J. J., Erickson, S. A., Flynn,et al. (2017). Natural polyreactive IgA antibodies coat the intestinal microbiota. Science, 358(6361), eaan6619.
  3. Solanas, G., Peixoto, F. O., Perdiguero, et al. (2017). Aged stem cells reprogram their daily rhythmic functions to adapt to stress. Cell, 170(4), 678-692.
  4. Sykes, D. B., Kfoury, Y. S., Mercier, F. Eet al. (2016). Inhibition of dihydroorotate dehydrogenase overcomes differentiation blockade in acute myeloid leukemia. Cell, 167(1), 171-186.
  5. Miyamoto T, Okano S, Kasai N. (2009) Irreversible thermoinactivation of ribonuclease-A by soft-hydrothermal processing. Biotechnol Prog. 25:1678-1685.
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