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Nucleic acid isolation is one of the most fundamental steps in molecular biology applications and is a prerequisite for many experiments. MagJET nucleic acid purification kits utilize magnetic bead–based technology, allowing for highly efficient nucleic acid isolation from a variety of samples at any throughput. MagJET kits provide high-purity DNA and RNA ready to use in routine and demanding downstream applications.
MagJET magnetic bead-based technology utilizes proprietary high capacity paramagnetic particles optimized to isolate nucleic acids with superior purity and yields compared to other kits on the market. Due to large total surface area, MagJET magnetic beads exhibit high binding capacity, resulting in superior nucleic acid yields and recovery rates typically exceeding 80%. Uniform bead size and shape ensure robust performance and consistent results with less sample-to-sample variation. MagJET magnetic beads are highly stable in solution and respond rapidly to an applied magnetic field making MagJET nucleic acid purification kits an ideal choice for high-throughput automation.
MagJET nucleic acid isolation technology is suitable both for high-throughput automated and manual sample processing.
(A) The automation protocols are optimized for Thermo Scientific Kingfisher instruments where magnetic beads are transferred from well to well during the purification procedure.
(B) In the manual protocol a magnetic field applied by an external source is used to capture the beads against the wall of the tube. The supernatant is removed by aspiration.
Manual and automated workflows were used to purify RNA from N. tabacum leaf samples (each 50 mg) with the MagJET Plant RNA Kit. The Thermo Scientific KingFisher protocol was used for the automated workflow. Isolated RNA was analyzed by agarose gel electrophoresis (1%), M – Thermo Scientific RiboRuler High Range RNA Ladder.
MagJET magnetic bead-based technology utilizes proprietary high capacity paramagnetic particles optimized to isolate nucleic acids with superior purity and yields compared to other kits on the market. Due to large total surface area, MagJET magnetic beads exhibit high binding capacity, resulting in superior nucleic acid yields and recovery rates typically exceeding 80%. Uniform bead size and shape ensure robust performance and consistent results with less sample-to-sample variation. MagJET magnetic beads are highly stable in solution and respond rapidly to an applied magnetic field making MagJET nucleic acid purification kits an ideal choice for high-throughput automation.
MagJET nucleic acid isolation technology is suitable both for high-throughput automated and manual sample processing.
(A) The automation protocols are optimized for Thermo Scientific Kingfisher instruments where magnetic beads are transferred from well to well during the purification procedure.
(B) In the manual protocol a magnetic field applied by an external source is used to capture the beads against the wall of the tube. The supernatant is removed by aspiration.
Manual and automated workflows were used to purify RNA from N. tabacum leaf samples (each 50 mg) with the MagJET Plant RNA Kit. The Thermo Scientific KingFisher protocol was used for the automated workflow. Isolated RNA was analyzed by agarose gel electrophoresis (1%), M – Thermo Scientific RiboRuler High Range RNA Ladder.
The MagJET Plasmid DNA Kit is ideal for high-throughput plasmid DNA miniprep isolation from 0.5 to 5 mL of bacterial culture. Isolated plasmid DNA is compatible with sensitive downstream applications such as PCR, restriction enzyme digestion and transfection of robust cell lines.
MagJET Genomic DNA Purification Kits are suitable for high-throughput genomic DNA purification and are compatible with a large variety of organisms and cell types, including eukaryotic cells, tissue culture, blood, plants, bacteria and yeast.
MagJET RNA Purification Kits deliver high total RNA yields and exceptional purity. This allows for reliable use of isolated RNA in every demanding downstream application such as RT-PCR, RT-qPCR and library preparation.
MagJET Viral DNA and RNA Kit allows for simultaneous viral nucleic acid purification from human plasma, serum, cell swabs, urine and other bodily liquids. The kit is ideal for high-throughput sample processing delivering consistent yields and robust performance.
The MagJET mRNA Enrichment Kit allows for fast and efficient purification of mRNA from a broad range of total RNA amounts. High binding capacity, uniform particle size, and rapid magnetic response of MagJET Oligo (dT) beads makes the technology ideal for high- and low-throughput purification.
The MagJET NGS Cleanup and Size Selection Kit is optimized for robust and efficient DNA fragment library purification and size selection from a large variety of enzymatic reaction mixtures used in NGS workflows.
A magnetic separation racks intended for low-throughput magnetic bead manipulation procedures such as magnetic bead-based nucleic acid isolation.