Product | Characteristics | Selected applications |
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Dynabeads M-270 Epoxy | - Hydrophilic bead surface coating with low non-specific protein binding
- Freeze dried monosized beads; easy to disperse in buffer before use
- Size: 2.8 micrometer
- Active epoxy groups for direct covalent coupling to antibodies, antigens or other biomolecules; no further surface activation required
- Low aggregation
| - Immunoassays and immunodiagnostics
- Protein and peptide purification
- Immunoprecipitation
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Dynabeads MyOne Epoxy | - Bead surface chemistry optimized for enhanced binding capacity and low non-specific binding
- Freeze dried monosized beads, easy to disperse in buffer before use
- Size: 1 micrometer
- Low sedimentation rate and higher reaction kinetics compared to M-270 beads
- Active epoxy groups for direct covalent coupling to antibodies, antigens or other biomolecules; no further surface activation required
| - Immunoassays and immunodiagnostics
- Protein and peptide purification
- Immunoprecipitation
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Dynabeads MyOne Tosylactivated | - Hydrophobic bead surface
- Size: 1 micrometer, monodisperse
- Iron content (ferrites): 26% (37%)
- Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
- Active chemical functionality: 0.04-0.06 mmol/g beads
- Direct covalent binding of proteins/peptides via primary amino- or sulfydryl groups; no further surface activation required
| - Immunoassays and immunodiagnostics
- Protein and peptide purification
- Phage display and biopanning
- Cell separation
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Dynabeads M-280 Tosylactivated | - Hydrophobic bead surface with p-toluene-sulfonyl (tosyl) groups
- Size: 2.8 micrometer, monodisperse
- Iron content (ferrites): 12% (17%)
- Active chemical functionality: 0.1-0.2 mmol/g beads
- Direct covalent binding of proteins/peptides via primary amino- or sulfydryl groups; no further surface activation required
| - Immunoassays and immunodiagnostics
- Protein and peptide purification
- Phage display and biopanning
- Cell separation
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Dynabeads MyOne Streptavidin T1 | - Hydrophobic bead surface
- Based on tosylactivated beads (T1)
- Size: 1 micrometer, monodisperse
- BSA as blocking protein
- Isoelectric point: pH 5.0
- Low charge (-10 mV, at pH 7)
- Iron content (ferrites): 26% (37%)
- Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
- Free biotin binding: > 1,100-1,700 pmoles/mg beads
- Binding biotinylated Ig: 40-50 µg/mg beads
| - Immunoassays and immunodiagnostics
- Isolation of biotinylated molecules
- Protein and peptide purification
- Phage display and biopanning
- Cell separation
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Dynabeads M-280 Streptavidin
| - Hydrophobic bead surface
- Based on tosylactivated beads
- Size: 2.8 micrometer, monodisperse
- BSA as blocking protein
- Isoelectric point: pH 5.0
- Low charge (-10 mV, at pH 7)
- Iron content (ferrites): 12% (17%)
- Free biotin binding: 650-900 pmoles/mg beads
- Binding biotinylated Ig: 5-10 µg/mg beads
| - Immunoassays and immunodiagnostics
- Isolation of biotinylated molecules
- Protein and peptide purification
- Phage display and biopanning
- Cell isolation
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Dynabeads M-280 Sheep anti-Rabbit IgG | - Hydrophobic bead surface
- Coated with affinity purified sheep polyclonal against all rabbit IgG subclasses
- Size: 2.8 micrometer, monodisperse
- Iron content (ferrites): 12% (17%)
- Mostly Fc reactive
- Binding capacity: 0.1-1 µg Ig/107 beads
| - Immunoassays and immunodiagnostics
- Protein and peptide purification
- Phage display and biopanning
- Cell isolation
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Dynabeads M-270 Streptavidin | - Hydrophilic bead surface
- Based on carboxylic acid beads
- Size: 2.8 micrometer, monodisperse
- No blocking proteins used
- Isoelectric point: pH 4.5
- Highly charged (-50 mV, at pH 7)
- Iron content (ferrites): 14% (20%)
- Low aggregation of beads in high salt solutions
- Free biotin binding: 650-1,350 pmoles/mg beads
- Binding biotinylated Ig: 5-10 µg/mg beads
| - Immunoassays and immunodiagnostics with hydrophobic targets
- Isolation of biotinylated molecules
- Sequence specific DNA/RNA capture in nucleic acid based diagnostics
- For use in protocols that require GTC lysis or high salt concentrations, or protocols not compatible with BSA.
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Dynabeads M-270 Carboxylic Acid | - Hydrophilic bead surface with carboxylic acid groups
- Size: 2.8 micrometer, monodisperse
- Iron content (ferrites): 14% (20%)
- Very low aggregation
- Active chemical functionality 150 µmol/g beads
| - Immunoassays and immunodiagnostics
- Sequence specific DNA/RNA capture in nucleic acid based diagnostics
- Immobilization of amino-modified oligonucleotides, antibodies or other ligands, for capture of specific DNA/RNA sequences, proteins or any other analyte
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Dynabeads MyOne Streptavidin C1
| - Hydrophilic bead surface
- Based on carboxylic acid beads (C1)
- Size: 1 micrometer, monodisperse
- No blocking proteins used
- Tween 20 in the buffer
- Isoelectric point: pH 5.2
- Medium charged (-35 mV, at pH 7)
- Iron content (ferrites): 26% (37%)
- Low sedimentation rate and improved reaction kinetics compared to M-280/M-270 beads
- Very low aggregation
- Free biotin binding: > 2,500 pmoles/mg beads
- Binding biotinylated Ig: 20-25 µg/mg beads
| - Immunoassays and immunodiagnostics
- Isolation of biotinylated molecules
- Sample preparation of proteins for mass spectrometry
- Sequence specific DNA/RNA capture in nucleic acid based diagnostics
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