Cryo-EM Sample Preparation

Cryo-EM sample preparation techniques

High-quality protein preparation is the foundation of any successful structural biology technique. For cryo-electron microscopy (cryo-EM), biophysical aspects such as composition, purity, homogeneity, and stability, as well as biochemical activity strongly contribute to preparation of good quality cryo-EM grids, but they can also significantly impact the resolution of the subsequent computational reconstruction. Starting with protein expression, followed by solubilization and stabilization of the expressed protein, with subsequent purification and clean-up, we offer a wide range of solutions to achieve the highest sample quality before vitrification of the specimen on the EM grid.