Human Hepatocytes

Fully-characterized to help maximize your results

High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology, increase the ability to draw in vitro/in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with relevant release specifications help ensure you receive the highest quality cryopreserved hepatocytes.

• Spheroid-Qualified Hepatocytes
• Hepatocyte pools
• Induction Qualified Cryopreserved
  
• Transporter Qualified Cryopreserved - Suspended and Plateable
  
• Metabolism Qualified Cryopreserved - Suspended and Plateable
  

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NEW:  See how our R&D team is transfecting cryopreserved human and fresh rat hepatocytes

• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

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To help facilitate your experiments, each lot of hepatocytes is qualified for particular applications:

• Suspension hepatocytes - suitable for most short term metabolism studies
• Plateable hepatocytes - useful for induction, plated metabolism, and transporter applications

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Gibco Human Spheroid-Qualified Hepatocytes

Our Spheroid qualified hepatocytes are the first off-the-shelf cryopreserved human cells for generation of hepatic spheroids in your own lab. Each vial contains a minimum of five million viable cells, sufficient for up to 16, 96-well plates of hepatic spheroids.

Get the convenience, affordability, and power of a pooled population of hepatocytes in a single vial. Our high-viability, metabolically active pooled human hepatocytes are a representative population of the different donor hepatocytes that have been isolated using our novel patent-pending CellStream Isolation Technology™, helping to deliver consistent, predictable results in your drug metabolism studies and other biological assays.

Our pools are useful in a wide variety of applications, including intrinsic clearance, transport, toxicology, biochemistry, and cell biology studies.

Using hepatocyte pools offers more consistent results by minimizing single donor and lot-to-lot variation. Furthermore, studies indicate the metabolic activity of a pool is representative of the population average.

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Our 5-Donor Human Plateable Hepatocytes are suitable for plated metabolism applications continuing >5 days and offer:

• High-viability, plateable hepatocytes
• Lot-specific characterization data
• Thawing and plating process compatible with existing protocols

Our 50-donor pooled hepatocytes contain 25 male and 25 female donors in each vial, and our 10-donor pooled hepatocytes contain 5 male and 5 female donors in each vial. Both products are suitable for short-term metabolism applications and offer:

• High-viability, plateable hepatocytes
• Hepatocytes isolated using our CellStream Isolation Technology™
• Verified enzymatic and pathway activity

Cryopreserved human hepatocytes for use in plated and suspension metabolism applications.  Each lot is assessed for the ability to express functional active uptake transport.  For suspension cryopreserved hepatocytes, the oil spin method is used.  Taurocholate, digoxin, and estradiol 17β glucuronide (E2-17G), substrates of the uptake and efflux transporters NTCP and BSEP, OATP1B3 and MDR1, OATPs and MRP2.

Cryopreserved human hepatocytes for use in plated and suspension metabolism applications. Prototypical cytochrome P450 substrates midazolam, tolbutamide, and dextromethorphan were used to assess the enzymatic function of CYP3A4⁄5, CYP2C9 and CYP2D6 respectively, for plated hepatocytes.  For suspension lots, each lot is characterized for CYP1A2, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1, CYP3A4, CYP3A4/5, FMO activity. HEP10(TM) Pooled Lots are further characterized for NTCP, OATP1B3, and OATP transporter pathways.