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Gibco Gene Therapy for Bioprocessing
Gene therapy is an exciting industry that is progressing rapidly with many scientists and researchers looking to commercialize biologics for therapeutic solutions. Our Gibco media can provide support as you scale up production of viral vector-based molecules for use in your therapeutic strategies. We are well-equipped, as pioneers and leaders in media manufacturing, scalable solutions, and format conversion to help efficiently meet your needs no matter the scale.
Gibco Bacto CD Supreme Fermentation Production Medium (FPM)
Achieve the next level in performance efficiency with the Gibco Bacto CD Supreme FPM, which offers a commercially available, chemically defined media to support robust plasmid production. Bacto CD Supreme Medium can help you improve lot-to-lot consistency, reduce handling, raw material management, and weighing and blending time, and help decrease the risk of contamination.
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Gibco F17 Expression Medium
With years of proven results, the Gibco F17 Expression Medium has become a standard in the gene therapy industry. This chemically defined, protein-free, serum-free medium was developed to support the growth and transfection of suspension HEK293 cells. 293-F, 293-H, and FreeStyle 293-F cells in suspension can be cultured directly into FreeStyle F17 Expression Medium without adaptation.
HEK293T AAV8 viral genome titer and percentage full capsids. (A) Panel media 1 and 5 yielded comparable average titers when evaluated with FreeStyle F17 Expression Medium. (B) Analysis of the average percentage of full capsids showed that panel medium 1 produced 76% full capsids compared to 51% with FreeStyle F17 Expression Medium and 40% with panel medium 5. (Testing was conducted in biological triplicate.)
Viral Production Medium, AGT
Gibco Viral Production Medium, AGT, is the dry-format version of CTS Viral Production Medium, a chemically defined, serum-free, protein-free, animal origin-free, phenol red-free medium that is part of the CTS AAV-MAX Helper-Free AAV Production System for high-titer adeno-associated virus (AAV) production.
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The Gibco Viral Vector HEK Media Panel
The Gibco Viral Vector HEK Media Panel provides a diverse offering to help accelerate gene therapy development and production. Covering a broad variety of HEK293 cell types and not tied to specific transfection reagents or techniques, the ready-to-use formulations will help you identify an optimal formulation for target cell lines and scale up quickly.
Gibco Viral Vector HEK Feed Panel
The HEK Feed Panel provides a diverse offering of formulations to help optimize your viral vector processes. This library of feeds is designed for HEK cells and has been tested in multiple media, including the Gibco Viral Vector HEK Media Panel. The feeds included in the panel can help meet your cells’ nutritional needs for you to scale-up production.
Scaling up with the HEK Feed Panel can provide:
- Diversity—distinct formulations for various cell lineages
- Simplicity—three ready-to-use, concentrated feed formulations
- Productivity—potential titer improvement over batch
- Expertise—dedicated field application scientist support through evaluation for customization, experimental design, and troubleshooting
Click the links to request more information on the Panel or download the new Gibco Viral Vector HEK Feed Panel Flyer
Gibco Bacto CD Supreme Fermentation Production Medium (FPM)
Achieve the next level in performance efficiency with the Gibco Bacto CD Supreme FPM, which offers a commercially available, chemically defined media to support robust plasmid production. Bacto CD Supreme Medium can help you improve lot-to-lot consistency, reduce handling, raw material management, and weighing and blending time, and help decrease the risk of contamination.
Click to enlarge image
Gibco F17 Expression Medium
With years of proven results, the Gibco F17 Expression Medium has become a standard in the gene therapy industry. This chemically defined, protein-free, serum-free medium was developed to support the growth and transfection of suspension HEK293 cells. 293-F, 293-H, and FreeStyle 293-F cells in suspension can be cultured directly into FreeStyle F17 Expression Medium without adaptation.
HEK293T AAV8 viral genome titer and percentage full capsids. (A) Panel media 1 and 5 yielded comparable average titers when evaluated with FreeStyle F17 Expression Medium. (B) Analysis of the average percentage of full capsids showed that panel medium 1 produced 76% full capsids compared to 51% with FreeStyle F17 Expression Medium and 40% with panel medium 5. (Testing was conducted in biological triplicate.)
Viral Production Medium, AGT
Gibco Viral Production Medium, AGT, is the dry-format version of CTS Viral Production Medium, a chemically defined, serum-free, protein-free, animal origin-free, phenol red-free medium that is part of the CTS AAV-MAX Helper-Free AAV Production System for high-titer adeno-associated virus (AAV) production.
Learn more ›
The Gibco Viral Vector HEK Media Panel
The Gibco Viral Vector HEK Media Panel provides a diverse offering to help accelerate gene therapy development and production. Covering a broad variety of HEK293 cell types and not tied to specific transfection reagents or techniques, the ready-to-use formulations will help you identify an optimal formulation for target cell lines and scale up quickly.
Gibco Viral Vector HEK Feed Panel
The HEK Feed Panel provides a diverse offering of formulations to help optimize your viral vector processes. This library of feeds is designed for HEK cells and has been tested in multiple media, including the Gibco Viral Vector HEK Media Panel. The feeds included in the panel can help meet your cells’ nutritional needs for you to scale-up production.
Scaling up with the HEK Feed Panel can provide:
- Diversity—distinct formulations for various cell lineages
- Simplicity—three ready-to-use, concentrated feed formulations
- Productivity—potential titer improvement over batch
- Expertise—dedicated field application scientist support through evaluation for customization, experimental design, and troubleshooting
Click the links to request more information on the Panel or download the new Gibco Viral Vector HEK Feed Panel Flyer
Gibco Media Manufacturing Services
With over 60 years of experience developing industry-leading media, the Gibco team can help you achieve optimal performance and accelerate your gene therapy development and production. As a leader in quality, innovation, and capacity, we are equipped to efficiently meet your demand, no matter the scale. With expertise in media manufacturing, scalable solutions, and format conversion, our proven team and leading services can help enhance your workflows. From non-GMP media prototyping to manufacturing your own formulation for production and custom packaging, we can support your entire media manufacturing journey.
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Related resources
Technical resources
Application note: Viral Vector HEK Media Panel addresses HEK293 cell lineage diversity in AAV production through basal screening
Articles: Media panels for AAV manufacturing: Finding the formulation for success
Articles: Prepare for progress: Streamlining the transition to clinical trials
Articles: Reducing animal-origin components to maximize AAV process sustainability
Brochure: Gibco PD-Express Services
Brochure: Scaling up with Gibco media
Flyer: Your cell line is unique. Your medium should be too.
Poster: Addressing HEK293 cell lineage diversity through basal media optimization
Technical literature: Quality by design for AAV production: a fast track to AAV manufacturing success
Whitepaper: Taking a leaf out of the mAb manufacturing book: media panels for AAV production
Bioprocessing resources
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