Research applications and techniques for cell culture

Cell culture application notes

Cell Culture & Transfection application notes present research applications and proof-of-concept experiments involving various products from our cell biology portfolio, including Thermo Scientific, Invitrogen and Gibco products.

If you're looking to learn about methods and techniques to achieve improved transfection and cell culture results, tested protocols, or descriptions of various techniques (and their tradeoffs), you are likely to find such answers among these application notes.

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TitleApplication
Generation of MCF7 spheroids in serum-free conditions 3D culture
Harnessing new dimensions in your research: coming ’round to spheroid culture 3D culture
I want to keep my samples at a temperature of 37°C. Which type of water bath is ideal for this requirement: general purpose, circulating or shaking? Baths, circulators, chillers
I want to use a bath circulator to immerse my vessels. How do I select the right size bath for my application?
EN  | ES  | DE 		Baths, circulators, chillers
How do I select an energy efficient refrigerated bath circulator? Baths, circulators, chillers
How do I ensure that my heated bath circulator will be able to control to that temperature rate? Baths, circulators, chillers
How do I achieve the best (fastest) time-to-temperature from a heated bath circulator? Baths, circulators, chillers
What steps can I take to ensure that my heated bath circulator can actually bring my application to setpoint temperature? Baths, circulators, chillers
Does a larger fluid reservoir give me more cooling capacity and improved temperature stability? EN  | ES  | DE Baths, circulators, chillers
How will the cost of purchasing and running a recirculating chiller or bath circulator affect my bottom line? Baths, circulators, chillers
I plan on using my bath circulator for external circulation only. Should I get a large reservoir that can absorb the heat for more stability? EN  | ES  | DE Baths, circulators, chillers
University of Michigan field study of Class II Biological Safety Cabinet energy consumption costs Biological safety cabinets
Ergonomics and safety in biological research cabinets Biological safety cabinets
Why is a dual blower system better than a single blower system? Biological safety cabinets
Thermo Scientific Biosafety: A European view Biological safety cabinets
How can you extend the filter life of your BSC? Biological safety cabinets
Why should you select a Class II Biological Safety Cabinet (BSC) with a DC motor over one with an AC motor? Biological safety cabinets
Where is the highest risk of contamination in your biological safety cabinet (BSC): the HEPA filters or the airflow balance at the front opening? Biological safety cabinets
Why are airflow alarms an important consideration for biological safety cabinets (BSCs) Biological safety cabinets
Biological safety cabinet filter testing—Lessons from overseas Biological safety cabinets
ECM coating protocol for Lab Tek and Lab Tek II Cellular Imaging
How is it possible to reduce energy consumption and environmental impact with production-level centrifuges? Centrifugation
How can I help improve safety in my laboratory when spinning pathogens or patient samples in swing-out rotors? Centrifugation
How can instant rotor identification enhance productivity and safety during your centrifuge set-up and run? Centrifugation
Separation of PBMCs from blood samples using the new Thermo Scientific benchtop 1L centrifuge Centrifugation
How can blood banking and bioprocessing production facilities enhance ergonomics, while simplifying operation for greater productivity during their separations? Centrifugation
Large volume precipitation of proteins with ammonium sulfate using Thermo Scientific Fiberlite Carbon Fiber Rotors  Centrifugation
Large-scale recombinant protein production using the Thermo Scientific Sorvall LYNX 6000 Superspeed Centrifuge and Fiberlite Large Capacity Rotors  Centrifugation
Isolation of mitochondria using the Thermo Scientific Sorvall LYNX 6000 superspeed centrifuge  Centrifugation
How does Thermo Scientific Auto-Lock rotor exchange facilitate rotor placement and improve safety, compared with traditional rotor tie-down systems? EN  | DE Centrifugation
Pelleting efficiency of patented tilted adapters using the new Thermo Scientific benchtop 1L centrifuges: a comparison of pelleting in Thermo Scientific benchtop and superspeed floor-model centrifuges  Centrifugation
What technologies make it possible to reduce energy consumption and environmental impact with high performance floor model centrifuges? Centrifugation
Why does the location of sensors in my CO2 incubator affect responses from my cultured cells? CO2 incubation
Eliminate incubator contamination with Thermo Scientific Heracell incubators  CO2 incubation
Importance of Class 100 air in a CO2 incubator  CO2 incubation
Effective heat sterilization in CO2 incubators  CO2 incubation
5 Reasons to enhance your cell culture using variable oxygen control  CO2 incubation
Which incubation parameters are most important for proper cell growth and expression? CO2 incubation
Proper care and maintenance of your cell culture incubator CO2 incubation
Decontamination cycles in BBD 6220 and Heracell incubators completely eliminate Mycoplasma  CO2 incubation
How can using the wrong type of water to provide in-chamber humidity cause corrosion in my CO2 incubator? CO2 incubation
Why is automated heat decontamination a better solution for sterilizing your CO2 incubator than an automated chemical disinfection?  CO2 incubation
Cell culture contamination: The role of the CO2 incubator in reducing contamination  CO2 incubation
Why is a circulating fan essential to superior growth conditions in a CO2 cell culture incubator? CO2 incubation
Oxygen: Too much of a good thing?  CO2 incubation
Why is a circulating fan essential to superior growth conditions in a cell culture incubator?  CO2 incubation
Back to basics: Proper care and maintenance for your cell culture incubator  CO2 incubation
I’m hearing a lot about SNAP in my lab. What is SNAP? Cold storage
I’ve heard that switching to −70°C for ultra-low freezers can reduce energy, but is that safe for my sample? Cold storage
I need an ultra-low freezer to store my samples. Does the initial pull down time of a freezer indicate how well the unit can protect my samples after a door opening? Cold storage
What do I need to consider when preparing samples in bags using a controlled-rate freezer? Cold storage
What kind of storage tube should I use to prevent the breakdown of RNA in tissue during cold storage? Cold storage
How can a water-cooled condenser option significantly reduce energy consumption and heat output without compromising sample protection? Cold storage
Is my -80°C freezer designed to prepare samples for long-term storage? Cold storage
Is the use of hydrocarbon refrigerants in laboratory refrigerators and freezers safe? Cold storage
Is there a safer way to store potentially flammable, pharmaceutical-grade materials that require refrigerated or frozen temperatures? Cold storage
Cryopreservation of mammalian cells  Cryopreservation
Edge 2.0 Plate Application Note Cultureware
pH and pressure issues in closed cell culture vessels Cultureware
Application properties of materials used for porous membranes in cell culture inserts  Cultureware
How does a low-binding culture surface compare to a non-treated surface in supporting cancer spheroid cultures? Cultureware
Uneven cell growth during incubation  Cultureware
Cell harvesting by temperature reduction—Nunc UpCell Surface Cultureware
Feeder cell and extracellular matrix-free cultivation of hPSC on Nunclon Vita with Rho-kinase inhibitor  Cultureware
Compatibility of mounting media on Nunc Permanox Slides  Cultureware
Reduced evaporation in Nunc Edge plate with solid reservoir  Cultureware
Superior imaging quality of Nunc Optical Bottom Plate  Cultureware
The Thermo Scientific Carrier Plate enables precise adjustment of height for Cell Culture Inserts in multiwell dishes  Cultureware
The Thermo Scientific Nunclon Sphera surface supports effective formation of three dimensional cancer spheroids in suspension Cultureware
Thermo Scientific Nunc TripleFlask culturing technique  Cultureware
Flow rate and throughput of cell culture media and serum using Nalgene Rapid-Flow filters with 0.2 micron PES membrane  Cultureware
Culturing embryonic stem cells using media filtered with Thermo Scientific Nalgene Rapid-Flow PES filter units  Filtration
Culturing cells under hypoxic conditions for biologically relevant resultsFiltration
Enhanced expansion of neural stem cells using a heat stable bFGF General cell culture
Induction of cancer cell migration using a heat stable bFGF General cell culture
Enhanced proliferation of primary NSCs and sustained differentiation into precursors using heat-stable bFGF General cell culture
Optimizing neuron adhesion and growth by choosing the right surface of Thermo Scientific Chamber Slide General cell culture
Cell culture contamination: Understanding the causes and managing the risks  General cell culture
Choosing the right orbital shaker for your application Orbital shakers
How does a CO2 resistant shaker provide greater reliability, flexibility and ease of use for varied cell culture applications, compared to a dedicated incubated CO2 shaker? Orbital shakers
Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in StemFlex MediumTransfection
Transfection of pluripotent stem cells with Lipofectamine Stem reagent in mTeSR1 Medium on Geltrex matrixTransfection
Transfection of neural stem cells with Lipofectamine Stem Transfection Reagent in StemPro mediumTransfection
iPSC generation using Lipofectamine transfection reagent Transfection
Transfection of pluripotent stem cells with Lipofectamine Stem Transfection Reagent in Essential 8 MediumTransfection
Delivery solutions for immune cellsTransfection
Neurobiology transfection guideTransfection
Improve genome editing outcomes in biologically relevant cell modelsTransfection
Generation and transfection of induced pluripotent stem cells (iPSCs)Transfection
Improve lentiviral production using Lipofectamine 3000 reagentTransfection
Resources

Cell Culture & Transfection Learning Center
Access cell culture and transfection educational resources for better experiment planning and execution.

Media Formulation Tool
Find the right Gibco media formulation for DMEM, DMEM/F-12, MEM, and RPMI-1640 media.

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Find technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.

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