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T25 Flasks for Cell Culture
T25 flask dimensions
Start your scale-up with a T25 flask that has the surface and ancillary options you're looking for in a tissue culture flask. Choose a cell culture flask from a collection that includes straight-neck or angled-neck options and surface treatment alternatives that help ensure your healthy tissue cells expand and grow in the flask of your choice.
Some useful numbers such as surface area and volumes of dissociation solutions are given below for T25 flasks.
Please note: The number of cells on a flask will vary with cell type. For this table, 0.7 x 106 HeLa cells were seeded and the confluent flask contained 2.8 x 106 cells.
| T25 flasks have a surface area of 25 cm2 | ||
|---|---|---|
| Dissociation reagent | Volume of reagent | Volume of growth medium |
| TrypLE Express enzyme | 3.0 mL | 3–5 mL |
| TrypLE Select (1x) enzyme | 3.0 mL | |
| TrypLE Select (10x) enzyme | 0.3 mL diluted to 3.0mL in DPBS/1 mM EDTA | |
| Versene reagent | 3.0 mL | |
| Trypsin | 3.0 mL | |
T25 flasks: EasYFlask products
Ergonomically designed for maximal access to the growth surface, this low-profile flask optimizes incubator space and features angled walls for complete viewing under microscopes. Additional features include multiple cap options, and sterilization by gamma irradiation with non-pyrogenic certification.
T25 flask surfaces by cell culture types
To help ensure flexible, reproducible, and reliable results across every stage of cell culture, T25 flasks come in a range of formats, sizes, and surfaces. Please see below for guidance on product selection for specific cell types and desired cell culture ranges.
Untreated surfaces for non-adherent cell suspension cultures
Made with high-quality, optically clear virgin polystyrene, the Thermo Scientific Nunc non-treated flasks, with high-level sterility assurance, are ideal for suspension culture of non-adherent cells.
Nunclon Delta T25 surface treatments for adherent cells
Composed of cell culture-treated polystyrene and surface treated with proprietary Nunclon Delta surface, these T25 flasks support optimal growth and attachment of cells to the growth surface.
Filtered caps allow for constant airflow while vent/close caps allow for sufficient gas exchange.
Nunclon Sphera T25 surface treatments for spheroid, organoid, and 3D cell cultures
This ultra-low binding surface is designed to grow scaffold-free spheroid and organoid cultures and is ideally suited for cancer and stem cells.
Thermo Scientific BioLite T25 surface treatments for general cell culture research
Thermo Scientific BioLite cell culture treated flasks with filter cap and seal cap options suit a multitude of experimental needs while providing cost efficiency for a wide selection of applications in your cell culture routine.
Caps come in both vented and plug seal varieties.
T25 flasks with poly-d-lysine coating or collagen I coatings
Poly-D-lysine- (PDL) and collagen I-coated flasks are ideal for adherent cultures of primary cells that are finicky and have difficulties attaching to the growth surface.
PDL is an animal-free synthetic surface for adherent cultures or primary cells that are finicky and hard to attach, while collagen I, also excellent for finicky cells, is of animal origin. Selection of one surface over another depends on tissue type. Please review previous references for your specific cell line or, if necessary, test both.
Untreated surfaces for non-adherent cell suspension cultures
Made with high-quality, optically clear virgin polystyrene, the Thermo Scientific Nunc non-treated flasks, with high-level sterility assurance, are ideal for suspension culture of non-adherent cells.
Nunclon Delta T25 surface treatments for adherent cells
Composed of cell culture-treated polystyrene and surface treated with proprietary Nunclon Delta surface, these T25 flasks support optimal growth and attachment of cells to the growth surface.
Filtered caps allow for constant airflow while vent/close caps allow for sufficient gas exchange.
Nunclon Sphera T25 surface treatments for spheroid, organoid, and 3D cell cultures
This ultra-low binding surface is designed to grow scaffold-free spheroid and organoid cultures and is ideally suited for cancer and stem cells.
Thermo Scientific BioLite T25 surface treatments for general cell culture research
Thermo Scientific BioLite cell culture treated flasks with filter cap and seal cap options suit a multitude of experimental needs while providing cost efficiency for a wide selection of applications in your cell culture routine.
Caps come in both vented and plug seal varieties.
T25 flasks with poly-d-lysine coating or collagen I coatings
Poly-D-lysine- (PDL) and collagen I-coated flasks are ideal for adherent cultures of primary cells that are finicky and have difficulties attaching to the growth surface.
PDL is an animal-free synthetic surface for adherent cultures or primary cells that are finicky and hard to attach, while collagen I, also excellent for finicky cells, is of animal origin. Selection of one surface over another depends on tissue type. Please review previous references for your specific cell line or, if necessary, test both.
Related flask and cell culture resources
Surface selection: Achieving optimal cell growth and harvesting
Get an overview of technologies used to modify cultureware surfaces to help you select the right surface for your application.
- Surface modifications to improve cell adhesion
- Characterization of Nunc cell culture surfaces by adhesion properties
- Alternative methods for advanced cell harvesting
Establishing a dynamic micro-gravity spheroid 3D culture system
Learn about how to build specific 3D cell spheroid models and platforms to answer the pressing and relevant questions in cancer research.
- Discerning the advantages and disadvantages of 2D versus 3D cell culturing approaches
- Understanding the method of rotating micro-gravity bioreactors as a means to produce long-term spheroid cultures
- Implementing spheroid cultures in cancer research
Watch more cell culture webinars
Register for our next on-demand webinar event and sign up to receive everything we have on cell culture.
Cell adhesion and growth on coated or modified glass or plastic surfaces
Growth substrates affect the adhesion, growth, morphology and differentiation of various cell types. However, the response to a given surface is cell type specific. Selection of a growth surface for cell culture should be based on cell culture performance and on application. In this technical bulletin, the growth of various cell types on plastic, soda lime glass and borosilicate coverglass was examined with surfaces that were either unmodified, coated with polylysine, or stably surface modified with non-biological reagents or electrical discharge.
Are you on the front lines of cancer research?
Surface selection: Achieving optimal cell growth and harvesting
Get an overview of technologies used to modify cultureware surfaces to help you select the right surface for your application.
- Surface modifications to improve cell adhesion
- Characterization of Nunc cell culture surfaces by adhesion properties
- Alternative methods for advanced cell harvesting
Establishing a dynamic micro-gravity spheroid 3D culture system
Learn about how to build specific 3D cell spheroid models and platforms to answer the pressing and relevant questions in cancer research.
- Discerning the advantages and disadvantages of 2D versus 3D cell culturing approaches
- Understanding the method of rotating micro-gravity bioreactors as a means to produce long-term spheroid cultures
- Implementing spheroid cultures in cancer research
Watch more cell culture webinars
Register for our next on-demand webinar event and sign up to receive everything we have on cell culture.
Cell adhesion and growth on coated or modified glass or plastic surfaces
Growth substrates affect the adhesion, growth, morphology and differentiation of various cell types. However, the response to a given surface is cell type specific. Selection of a growth surface for cell culture should be based on cell culture performance and on application. In this technical bulletin, the growth of various cell types on plastic, soda lime glass and borosilicate coverglass was examined with surfaces that were either unmodified, coated with polylysine, or stably surface modified with non-biological reagents or electrical discharge.
Are you on the front lines of cancer research?
For Research Use Only. Not for use in diagnostic procedures.