Workflow Features

  • Ideal for quantitating small RNAs - all you need is the sequence of interest
  • Analyze any small nucleic acid less than 200 bases long, including newly discovered miRNAs, Piwi-interacting RNA (piRNA), small nuclear RNA (snRNA), and small nucleolar RNA (snoRNA)
  • Assays include both the Custom TaqMan® Small RNA Assay and a target-specific reverse transcription primer

See Literature/References

How It Works

Custom TaqMan® Small RNA Assays employ an innovative target-specific stem-loop reverse transcription primer to address the challenge of the short length of the small RNA. The primer extends the 3' end of the target to produce a template that can be used in standard TaqMan® Assay-based qPCR. Also, the stem-loop structure in the tail of the primer confers a key advantage if you are studying miRNAs: specific detection of the mature, biologically active miRNA.

Small RNA Analysis Experimental Workflow

Life Technologies offers a variety of kits for the isolation and analysis of miRNA and other small RNAs. Each is ideal for use in miRNA analysis because they enable quantitative recovery of small RNA (<200 nt) and maintenance of representative amounts of small RNA (eliminating experimental bias).

Recommended Products

 

mirVana™ miRNA Isolation Kit
Isolate total RNA that contains small RNAs such as miRNA, siRNA, and snRNA. These small RNAs are often lost during traditional RNA isolation methods. In addition, this kit includes reagents and instructions for the isolation of RNA that is enriched for small RNA (<200 bp), enabling more sensitive detection of low-level small RNAs.

 

RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE
Extract total RNA or DNA from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues. This kit enables the molecular analysis of archived tissue samples at both the genomic and gene expression levels. With this kit, nucleic acid is obtained that is suitable for RT-qPCR, mutation screening, and microarray analyses.

 

TaqMan® MicroRNA Cells-to-CT™ Kit
Featuring a unique method for lysing cultured cells while removing genomic DNA and preserving RNA integrity, the TaqMan® MicroRNA Cells-to-CT™ Kit makes it possible to perform miRNA expression profiling directly from cultured cells without RNA purification.

Two-step TaqMan® MicroRNA Assays, containing the TaqMan® Assay and reverse transcription primer specific for the target of interest, are designed for individual miRNA targets found in a wide variety of species including human, mouse, rat, Drosophila, C. elegans, and Arabidopsis. The TaqMan® MicroRNA Reverese Transcription Kit provides optimal TaqMan® MicroRNA Assay performance.

Recommended Products

 

TaqMan® MicroRNA Reverse Transcription Kit
The TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for optimal performance of TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan® MicroRNA Assay to convert miRNA to cDNA.

 

Custom TaqMan® Small RNA Assays
Order a Custom TaqMan® Small RNA Assay by first entering a sequence, then submitting the chosen target regions for assay design. Upon notification of successful assay design, simply add the desired custom assays to your shopping basket.

For the final quantitation step, the cDNA product generated in step 2 is mixed with TaqMan® Universal Master Mix II and added to the matched TaqMan® MicroRNA Assay.

Recommended Products

 

Custom TaqMan® Small RNA Assays
Order a Custom TaqMan® Small RNA Assay by first entering a sequence, then submitting the chosen target regions for assay design. Upon notification of successful assay design, simply add the desired custom assays to your shopping basket.

 

TaqMan® Universal Master Mix II
This kit contains components needed for all 5' nuclease assays using TaqMan® Assay-based PCR applications. This easy-to-use solution minimizes your assay set-up time and employs AmpliTaq Gold® DNA polymerase for enhanced performance.

Step 1 : Isolate RNA

Life Technologies offers a variety of kits for the isolation and analysis of miRNA and other small RNAs. Each is ideal for use in miRNA analysis because they enable quantitative recovery of small RNA (<200 nt) and maintenance of representative amounts of small RNA (eliminating experimental bias).

Recommended Products

 

mirVana™ miRNA Isolation Kit
Isolate total RNA that contains small RNAs such as miRNA, siRNA, and snRNA. These small RNAs are often lost during traditional RNA isolation methods. In addition, this kit includes reagents and instructions for the isolation of RNA that is enriched for small RNA (<200 bp), enabling more sensitive detection of low-level small RNAs.

 

RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE
Extract total RNA or DNA from formalin- or paraformalin-fixed, paraffin-embedded (FFPE) tissues. This kit enables the molecular analysis of archived tissue samples at both the genomic and gene expression levels. With this kit, nucleic acid is obtained that is suitable for RT-qPCR, mutation screening, and microarray analyses.

 

TaqMan® MicroRNA Cells-to-CT™ Kit
Featuring a unique method for lysing cultured cells while removing genomic DNA and preserving RNA integrity, the TaqMan® MicroRNA Cells-to-CT™ Kit makes it possible to perform miRNA expression profiling directly from cultured cells without RNA purification.

Step 2 : Reverse Transcribe RNA into cDNA

Two-step TaqMan® MicroRNA Assays, containing the TaqMan® Assay and reverse transcription primer specific for the target of interest, are designed for individual miRNA targets found in a wide variety of species including human, mouse, rat, Drosophila, C. elegans, and Arabidopsis. The TaqMan® MicroRNA Reverese Transcription Kit provides optimal TaqMan® MicroRNA Assay performance.

Recommended Products

 

TaqMan® MicroRNA Reverse Transcription Kit
The TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for optimal performance of TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan® MicroRNA Assay to convert miRNA to cDNA.

 

Custom TaqMan® Small RNA Assays
Order a Custom TaqMan® Small RNA Assay by first entering a sequence, then submitting the chosen target regions for assay design. Upon notification of successful assay design, simply add the desired custom assays to your shopping basket.

Step 3 : Set Up & Run qPCR Reactions

For the final quantitation step, the cDNA product generated in step 2 is mixed with TaqMan® Universal Master Mix II and added to the matched TaqMan® MicroRNA Assay.

Recommended Products

 

Custom TaqMan® Small RNA Assays
Order a Custom TaqMan® Small RNA Assay by first entering a sequence, then submitting the chosen target regions for assay design. Upon notification of successful assay design, simply add the desired custom assays to your shopping basket.

 

TaqMan® Universal Master Mix II
This kit contains components needed for all 5' nuclease assays using TaqMan® Assay-based PCR applications. This easy-to-use solution minimizes your assay set-up time and employs AmpliTaq Gold® DNA polymerase for enhanced performance.

Step 4 : Analyze Data

Literature

Brochures

Product Information