TaqMan Multiplex Real-Time PCR (qPCR) Solution | Thermo Fisher Scientific

TaqMan® Multiplex Real-Time (qPCR) Solutions

New multiplex qPCR solution helps you get more data from your samples

Multiplex qPCR is a simple, efficient, and cost effective solution for overcoming the challenges of limited samples and costly analysis. Successful multiplex qPCR enables the amplification of more than one target in a single reaction using different reporters with distinct fluorescent spectra and making it possible for you to use less of your precious samples in each experiment.

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We are proud to offer a complete multiplex qPCR solution that enables you to detect up to six targets for applications such as microbal pathogen detection and gene expression. Our solution includes dyes optimized to work together with minimal spectral overlap, room-temperature-stable qPCR mixes designed for multiplexing, and endogenous control assays. These reagents, combined with our custom services and qPCR instruments and software, help decrease the amount of time required to design and optimize your multiplex qPCR experiments.

Learn more about how to multiplex by viewing our Multiplex Optimization Guide (PDF) ›

TaqMan QSY and QSY2 probes flyer

Learn more about how our non-fluorescent QSY and QSY2 probes outperform the competition when scaling from singleplex to multiplex.

Benefits to multiplexing

Multiplex qPCR allows you to get more information out of a single reaction. This is particularly important when your sample input is limited or when you need to minimize sample-analysis costs.

Multiplexing provides a way to reduce reaction costs by as much as 40%. The relative costs for the multiplex reaction are much closer to those for a singleplex reaction and offer substantial savings when compared to the costs of performing six singleplex reactions independently.

Design your own multiplex qPCR primers and probes

The TaqMan Multiplex solution consists of multiple components:

1. TaqMan QSY and QSY2 probes

TaqMan QSY and QSY2 probes are available for optimal high-level multiplexing with best performance in up to 6-plex reactions.

2. Multiplexing qPCR master mix

Applied Biosystems TaqMan and TaqPath master mixes are optimized for multiplexing in a variety of qPCR applications and formulated to maximize target amplification within multiplex reactions. To enable multiplex assay flexibility, you can choose between formulations with various passive reference dyes options; including ROX, Mustang Purple, and formulations with no passive reference.

Applied Biosystems qPCR master mixes provide application-specific formulations for multiplexing, including:

Gene expression analysis

2-Step gene expression analysis
- TaqMan Fast Advanced Master Mix
- TaqMan Fast Advanced Master Mix, No UNG
1-Step gene expression analysis
- TaqMan Fast Virus 1-Step Master Mix
- TaqMan Fast Virus 1-Step Master Mix, No ROX

Genotyping and genetic variation analysis

Microbial and pathogen detection

Research applications
- DNA targets
  - TaqMan Fast Advanced Master Mix
  - TaqMan Fast Advanced Master Mix, No UNG
- RNA targets
  - TaqMan Fast Virus 1-Step Master Mix
  - TaqMan Fast Virus 1-Step Master Mix, No ROX
Clinical and biopharmaceutical applications
- DNA targets
  - TaqPath Bactopure Microbial Detection Master Mix
  - TaqPath Bactopure Microbial Detection Master Mix, No ROX
- RNA targets
  - TaqPath DuraPlex 1-Step RT-qPCR Master Mix
  - TaqPath DuraPlex 1-Step RT-qPCR Master Mix, No ROX

MicroRNA analysis

3. Pre-designed TaqMan assays for endogenous controls

TaqMan QSY assays use JUN and ABY dyes to measure the expression of the GAPDH and RNase P genes. These assays are also available in a primer-limited format for your convenience. Run control assays and experimental assays in the same well.

Developing and optimizing your multiplex reaction

Learn more about how to design your own multiplex reaction with our Multiplex Optimization Guide (PDF) ›

This up-to-date guide will help you get started with your multiplex experiments and features our latest Applied Biosystems products that are optimized for multiplexing, including QSY and QSY2 probes, MGB probes, TaqPath Duraplex master mix, and calibration plate options.