Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Tandem mass tags (TMT) are robust and efficient systems for multiplex, relative protein quantitation of up to 35 samples, derived from cells, tissues, or biological fluids. All mass tagging reagents within a label reagent set have the same nominal mass (i.e., they are isobaric) and chemical structure, composed of a reactive group, a spacer arm (mass normalizer), and a mass reporter. The isobaric nature of the TMT labeling reagents allow for concurrent identification and quantification of proteins in different samples using tandem mass spectrometry (MS).
A quick comparison of TMT vs. TMTpro label reagents is shown in Table 1.
Features | TMT | TMTpro |
---|---|---|
Reactivity | Amine-reactive Cysteine-reactive Carbonyl-reactive | Amine-reactive |
Multiplex | 2- to 11-plex | 16- to 35-plex |
Isotopes | 15N and 13C | 15N, 13C, and 2H |
Chemical structure |
|
|
Reporter ion masses | 126–131C | 126–135CD |
Applications |
|
The TMTpro label reagents have increased multiplex capability and can be used for concurrent protein mass spectrometry analysis of 16 to 35 biological samples.
TMTpro Label Reagents listed below are configured and specific for 32, 34, and 35 sample multiplexing.
All TMTpro label reagents for 16 to 35 multiplexing function with a high-resolution mass spectrometry instrument (Orbitrap series).
Learn more about TMTpro reagents
Multiplex Capability | Configuration | Product(s) Description | Format | Unit size | Cat. No. |
---|---|---|---|---|---|
32-plex | Matched set | TMTpro 32plex Label Reagent Matched Set | Lyophilized | 1 x 5 mg (per tag) | A40000839 |
Combine sets | TMTpro 16plex Deuterated Label Reagent Set | Lyophilized | 1 x 5 mg (per tag) | A40000817 | |
TMTpro 16plex Label Reagent Set | 1 x 5 mg (per tag) | A44520 | |||
34-plex | Combine matched set with standalone tags | TMTpro 32plex Label Reagent Matched Set | Lyophilized | 1 x 5 mg (per tag) | A40000839 |
TMTpro-134C and TMTpro-135CD Label Reagents | 1 x 5 mg (per tag) | A40000853 | |||
Combine sets with standalone tags | TMTpro 16plex Label Reagent Set | Lyophilized | 1 x 5 mg (per tag) | A44520 | |
TMTpro 16plex Deuterated Label Reagent Set | 1 x 5 mg (per tag) | A40000817 | |||
TMTpro-134C and TMTpro-135CD Label Reagents | 1 x 5 mg (per tag) | A40000853 | |||
35-plex | Combine sets and standalone tag | TMTpro 18plex Label Reagent Set | Lyophilized | 1 x 5 mg (per tag) | A52045 |
TMTpro 16plex Deuterated Label Reagent Set | 1 x 5 mg (per tag) | A40000817 | |||
TMTpro-135CD Label Reagent | 1 x 5 mg (per tag) | A40000818 |
Note: All products listed in the above table have amine-reactive chemistry to label all proteins and peptides.
The TMTpro label reagents have increased multiplex capability and can be used for concurrent protein mass spectrometry analysis of 16 to 35 biological samples.
TMTpro Label Reagents listed below are configured and specific for 16 and 18 sample multiplexing.
All TMTpro label reagents for 16 to 35 multiplexing function with a high-resolution mass spectrometry instrument (Orbitrap series).
Learn more about TMTpro reagents
Multiplex Capability | Configuration | Product(s) Description | Format | Unit size | Cat. No. |
---|---|---|---|---|---|
16-plex | Set | TMTpro 16-plex Label Reagent Set | Lyophilized | 1 x 5 mg (per tag) | A44520 |
1 x 0.5 mg (per tag) | A44521 | ||||
6 x 0.5 mg (per tag) | A44522 | ||||
Set | TMTpro 16plex Label Reagents in stabilized solution, 96-well plate | Liquid, 96-well plate | 6 x 50 µg (per tag) | A58334 | |
18-plex | Set | TMTpro 18-plex Label Reagent Set | Lyophilized | 1 x 5 mg (per tag) | A52045 |
6 x 0.5 mg (per tag) | A52047 | ||||
Set | TMTpro 18plex Label Reagents in stabilized solution, 96-well plate | Liquid, 96-well plate | 4 x 50 µg (per tag) | A58335 | |
Reagent set with standalone tags | TMTpro 16-plex Label Reagent Set | Lyophilized | 1 x 5 mg (per tag) | A44520 | |
TMTpro-134C and TMTpro-135N Label Reagents | 1 x 5 mg (per tag) | A52046 | |||
Reagent set with standalone tags | TMTpro 16-plex Label Reagent Set | Lyophilized | 6 x 0.5 mg (per tag) | A44522 | |
TMTpro-134C and TMTpro-135N Label Reagents | 6 x 0.5 mg (per tag) | A52048 |
Note: All products listed in the above table have amine-reactive chemistry to label all proteins and peptides.
The TMT label reagents have increased multiplex capability and can be used for concurrent protein mass spectrometry analysis of 2 to 11 biological samples.
TMT Label Reagents listed below are configured and specific for 10 and 11 sample multiplexing.
Learn more about TMT reagents
Multiplex Capability | Instrument Resolution | Compatible Instruments | Product(s) Description | Format | Unit size | Cat. No. |
---|---|---|---|---|---|---|
10-plex | High resolution | Orbitrap | TMT10plex Isobaric Mass Tagging Kit | Lyophilized | 30 reactions | 90113 |
TMT10plex Isobaric Label Reagent Set | Lyophilized | 1 x 0.8 mg (per tag) | 90110 | |||
3 x 0.8 mg (per tag) | 90111 | |||||
1 x 5 mg (per tag) | 90406 | |||||
8 x 0.2 mg (per tag) | 90309 | |||||
TMT 10plex Label Reagents in stabilized solution, 96-well plate | Liquid, 96-well Plate | 8 x 40 µg (per tag) | A58332 | |||
11-plex | High resolution | Orbitrap | TMT10plex Isobaric Label Reagent Set plus TMT11-131C Label Reagent | Lyophilized | 1 x 5 mg (per tag) | A34808 |
3 x 0.8 mg (per tag) | A37725 | |||||
TMT 11plex Label Reagents in stabilized solution, 96-well plate | Liquid, 96-well Plate | 8 x 40 µg (per tag) | A58333 | |||
TMT11-131C Label Reagent | Lyophilized | 1 x 5 mg | A34807 | |||
3 x 0.8 mg | A37724 |
Note: All products listed in the above table have amine-reactive chemistry to label all proteins and peptides.
The TMT label reagents have increased multiplex capability and can be used for concurrent protein mass spectrometry analysis of 2 to 11 biological samples.
TMT Label Reagents listed on below are configured and specific for 2 and 6 sample multiplexing.
The 2- and 6-plex label reagents on this tab are compatible with both nominal and high-resolution instruments.
Learn more about TMT reagents
Multiplex capability | Reactive chemistry | Product(s) Description | Unit size | Cat. No. |
---|---|---|---|---|
2-plex | Amine-reactive | TMTduplex Isobaric Mass Tagging Kit | 15 reactions | 90063 |
TMTduplex Isobaric Label Reagent Set | 5 x 0.8 mg | 90065 | ||
TMTduplex Isotopic Label Reagent Set | 5 x 0.8 mg | 90060 | ||
6-plex | Amine-reactive | TMTsixplex Isobaric Label Reagent Kit | 35 reactions | 90064 |
Contact us | 90064B | |||
TMTsixplex Isobaric Label Reagent Set | 1 x 0.8 mg (per tag) | 90061 | ||
2 x 0.8 mg (per tag) | 90062 | |||
5 x 0.8 mg (per tag) | 90066 | |||
2 x 5 mg (per tag) | 90068 | |||
Cysteine-reactive | iodoTMTsixplex Isobaric Mass Tagging Kit | 30 reactions | 90103 | |
iodoTMTsixplex Isobaric Label Reagent Set | 1 x 0.2 mg (per tag) | 90101 | ||
5 x 0.2 mg (per tag) | 90102 | |||
Carbonyl-reactive | aminoxyTMTsixplex Label Reagent Set | 1 x 0.2 mg (per tag) | 90401 | |
5 x 0.2 mg (per tag) | 90402 |
Additional TMT and TMTpro reagents
Learn more about Super Heavy TMT reagents
Application | Reactive chemistry | TMT Type | Product* | Format | Unit size | Cat. No. |
---|---|---|---|---|---|---|
LC-MS Method Development | Amine-reactive* | TMTpro | TMTpro Zero Label Reagent | Lyophilized | 1 x 5 mg | A44518 |
Lyophilized | 6 x 0.5 mg | A44519 | ||||
Specialized for Targeted Multiplexing | Super Heavy TMTpro Label Reagent | Lyophilized | 2 mg | A52040 | ||
LC-MS Method Development | TMT | TMT Zero Label Reagent | Lyophilized | 5 x 0.8 mg | 90067 | |
Specialized for Targeted Multiplexing | Super Heavy TMT Label Reagent | Lyophilized | 2 mg | A43073 | ||
LC-MS Method Development | Cysteine-reactive** | iodo TMTzero Label Reagent | Lyophilized | 5 x 0.2 mg | 90100 |
* Amine-reactive chemistry to label all proteins and peptides.
** Cysteine-reactive chemistry to label peptides with reduced cysteine (Cys) residues.
The AccelerOme TMT labeling kits enable multiplex relative quantitation by protein mass spectrometry. These kits are available in combo packs containing a sample prep module, a TMT module, and a peptide retention time calibration mixture.
All TMTpro label reagents for 16 to 35 multiplexing function with a high-resolution mass spectrometry instrument (Orbitrap series).
Learn more about AccelerOme reagents
Multiplex Capability | Product | Unit size | Cat. No. |
---|---|---|---|
11-plex | AccelerOme TMT11plex MS Sample Preparation Kit, 10–30 µg | 3 x 11 reactions | A50946 |
AccelerOme TMT11plex MS Sample Preparation Kit, 30–100 µg | 1 x 11 reactions | A50947 | |
3 x 11 reactions | A50948 | ||
16-plex | AccelerOme TMTpro 16plex MS Sample Preparation Kit, 10–30 µg | 2 x 16 reactions | A50949 |
AccelerOme TMTpro 16plex MS Sample Preparation Kit, 30–100 µg | 1 x 16 reactions | A50950 | |
2 x 16 reactions | A50951 |
Note: All products listed in the above table have amine-reactive chemistry to label all proteins and peptides.
The TMTpro label reagents have increased multiplex capability and can be used for concurrent protein mass spectrometry analysis of 16 to 35 biological samples.
TMTpro Label Reagents listed below are configured and specific for 32, 34, and 35 sample multiplexing.
All TMTpro label reagents for 16 to 35 multiplexing function with a high-resolution mass spectrometry instrument (Orbitrap series).
Learn more about TMTpro reagents
Multiplex Capability | Configuration | Product(s) Description | Format | Unit size | Cat. No. |
---|---|---|---|---|---|
32-plex | Matched set | TMTpro 32plex Label Reagent Matched Set | Lyophilized | 1 x 5 mg (per tag) | A40000839 |
Combine sets | TMTpro 16plex Deuterated Label Reagent Set | Lyophilized | 1 x 5 mg (per tag) | A40000817 | |
TMTpro 16plex Label Reagent Set | 1 x 5 mg (per tag) | A44520 | |||
34-plex | Combine matched set with standalone tags | TMTpro 32plex Label Reagent Matched Set | Lyophilized | 1 x 5 mg (per tag) | A40000839 |
TMTpro-134C and TMTpro-135CD Label Reagents | 1 x 5 mg (per tag) | A40000853 | |||
Combine sets with standalone tags | TMTpro 16plex Label Reagent Set | Lyophilized | 1 x 5 mg (per tag) | A44520 | |
TMTpro 16plex Deuterated Label Reagent Set | 1 x 5 mg (per tag) | A40000817 | |||
TMTpro-134C and TMTpro-135CD Label Reagents | 1 x 5 mg (per tag) | A40000853 | |||
35-plex | Combine sets and standalone tag | TMTpro 18plex Label Reagent Set | Lyophilized | 1 x 5 mg (per tag) | A52045 |
TMTpro 16plex Deuterated Label Reagent Set | 1 x 5 mg (per tag) | A40000817 | |||
TMTpro-135CD Label Reagent | 1 x 5 mg (per tag) | A40000818 |
Note: All products listed in the above table have amine-reactive chemistry to label all proteins and peptides.
The TMTpro label reagents have increased multiplex capability and can be used for concurrent protein mass spectrometry analysis of 16 to 35 biological samples.
TMTpro Label Reagents listed below are configured and specific for 16 and 18 sample multiplexing.
All TMTpro label reagents for 16 to 35 multiplexing function with a high-resolution mass spectrometry instrument (Orbitrap series).
Learn more about TMTpro reagents
Multiplex Capability | Configuration | Product(s) Description | Format | Unit size | Cat. No. |
---|---|---|---|---|---|
16-plex | Set | TMTpro 16-plex Label Reagent Set | Lyophilized | 1 x 5 mg (per tag) | A44520 |
1 x 0.5 mg (per tag) | A44521 | ||||
6 x 0.5 mg (per tag) | A44522 | ||||
Set | TMTpro 16plex Label Reagents in stabilized solution, 96-well plate | Liquid, 96-well plate | 6 x 50 µg (per tag) | A58334 | |
18-plex | Set | TMTpro 18-plex Label Reagent Set | Lyophilized | 1 x 5 mg (per tag) | A52045 |
6 x 0.5 mg (per tag) | A52047 | ||||
Set | TMTpro 18plex Label Reagents in stabilized solution, 96-well plate | Liquid, 96-well plate | 4 x 50 µg (per tag) | A58335 | |
Reagent set with standalone tags | TMTpro 16-plex Label Reagent Set | Lyophilized | 1 x 5 mg (per tag) | A44520 | |
TMTpro-134C and TMTpro-135N Label Reagents | 1 x 5 mg (per tag) | A52046 | |||
Reagent set with standalone tags | TMTpro 16-plex Label Reagent Set | Lyophilized | 6 x 0.5 mg (per tag) | A44522 | |
TMTpro-134C and TMTpro-135N Label Reagents | 6 x 0.5 mg (per tag) | A52048 |
Note: All products listed in the above table have amine-reactive chemistry to label all proteins and peptides.
The TMT label reagents have increased multiplex capability and can be used for concurrent protein mass spectrometry analysis of 2 to 11 biological samples.
TMT Label Reagents listed below are configured and specific for 10 and 11 sample multiplexing.
Learn more about TMT reagents
Multiplex Capability | Instrument Resolution | Compatible Instruments | Product(s) Description | Format | Unit size | Cat. No. |
---|---|---|---|---|---|---|
10-plex | High resolution | Orbitrap | TMT10plex Isobaric Mass Tagging Kit | Lyophilized | 30 reactions | 90113 |
TMT10plex Isobaric Label Reagent Set | Lyophilized | 1 x 0.8 mg (per tag) | 90110 | |||
3 x 0.8 mg (per tag) | 90111 | |||||
1 x 5 mg (per tag) | 90406 | |||||
8 x 0.2 mg (per tag) | 90309 | |||||
TMT 10plex Label Reagents in stabilized solution, 96-well plate | Liquid, 96-well Plate | 8 x 40 µg (per tag) | A58332 | |||
11-plex | High resolution | Orbitrap | TMT10plex Isobaric Label Reagent Set plus TMT11-131C Label Reagent | Lyophilized | 1 x 5 mg (per tag) | A34808 |
3 x 0.8 mg (per tag) | A37725 | |||||
TMT 11plex Label Reagents in stabilized solution, 96-well plate | Liquid, 96-well Plate | 8 x 40 µg (per tag) | A58333 | |||
TMT11-131C Label Reagent | Lyophilized | 1 x 5 mg | A34807 | |||
3 x 0.8 mg | A37724 |
Note: All products listed in the above table have amine-reactive chemistry to label all proteins and peptides.
The TMT label reagents have increased multiplex capability and can be used for concurrent protein mass spectrometry analysis of 2 to 11 biological samples.
TMT Label Reagents listed on below are configured and specific for 2 and 6 sample multiplexing.
The 2- and 6-plex label reagents on this tab are compatible with both nominal and high-resolution instruments.
Learn more about TMT reagents
Multiplex capability | Reactive chemistry | Product(s) Description | Unit size | Cat. No. |
---|---|---|---|---|
2-plex | Amine-reactive | TMTduplex Isobaric Mass Tagging Kit | 15 reactions | 90063 |
TMTduplex Isobaric Label Reagent Set | 5 x 0.8 mg | 90065 | ||
TMTduplex Isotopic Label Reagent Set | 5 x 0.8 mg | 90060 | ||
6-plex | Amine-reactive | TMTsixplex Isobaric Label Reagent Kit | 35 reactions | 90064 |
Contact us | 90064B | |||
TMTsixplex Isobaric Label Reagent Set | 1 x 0.8 mg (per tag) | 90061 | ||
2 x 0.8 mg (per tag) | 90062 | |||
5 x 0.8 mg (per tag) | 90066 | |||
2 x 5 mg (per tag) | 90068 | |||
Cysteine-reactive | iodoTMTsixplex Isobaric Mass Tagging Kit | 30 reactions | 90103 | |
iodoTMTsixplex Isobaric Label Reagent Set | 1 x 0.2 mg (per tag) | 90101 | ||
5 x 0.2 mg (per tag) | 90102 | |||
Carbonyl-reactive | aminoxyTMTsixplex Label Reagent Set | 1 x 0.2 mg (per tag) | 90401 | |
5 x 0.2 mg (per tag) | 90402 |
Additional TMT and TMTpro reagents
Learn more about Super Heavy TMT reagents
Application | Reactive chemistry | TMT Type | Product* | Format | Unit size | Cat. No. |
---|---|---|---|---|---|---|
LC-MS Method Development | Amine-reactive* | TMTpro | TMTpro Zero Label Reagent | Lyophilized | 1 x 5 mg | A44518 |
Lyophilized | 6 x 0.5 mg | A44519 | ||||
Specialized for Targeted Multiplexing | Super Heavy TMTpro Label Reagent | Lyophilized | 2 mg | A52040 | ||
LC-MS Method Development | TMT | TMT Zero Label Reagent | Lyophilized | 5 x 0.8 mg | 90067 | |
Specialized for Targeted Multiplexing | Super Heavy TMT Label Reagent | Lyophilized | 2 mg | A43073 | ||
LC-MS Method Development | Cysteine-reactive** | iodo TMTzero Label Reagent | Lyophilized | 5 x 0.2 mg | 90100 |
* Amine-reactive chemistry to label all proteins and peptides.
** Cysteine-reactive chemistry to label peptides with reduced cysteine (Cys) residues.
The AccelerOme TMT labeling kits enable multiplex relative quantitation by protein mass spectrometry. These kits are available in combo packs containing a sample prep module, a TMT module, and a peptide retention time calibration mixture.
All TMTpro label reagents for 16 to 35 multiplexing function with a high-resolution mass spectrometry instrument (Orbitrap series).
Learn more about AccelerOme reagents
Multiplex Capability | Product | Unit size | Cat. No. |
---|---|---|---|
11-plex | AccelerOme TMT11plex MS Sample Preparation Kit, 10–30 µg | 3 x 11 reactions | A50946 |
AccelerOme TMT11plex MS Sample Preparation Kit, 30–100 µg | 1 x 11 reactions | A50947 | |
3 x 11 reactions | A50948 | ||
16-plex | AccelerOme TMTpro 16plex MS Sample Preparation Kit, 10–30 µg | 2 x 16 reactions | A50949 |
AccelerOme TMTpro 16plex MS Sample Preparation Kit, 30–100 µg | 1 x 16 reactions | A50950 | |
2 x 16 reactions | A50951 |
Note: All products listed in the above table have amine-reactive chemistry to label all proteins and peptides.
The TMTpro 16-plex and 35-plex label reagents are the next generation of TMT reagents (TMT tags) that offer increased multiplex capability and sample throughput [1]. Concurrent protein mass spectrometry analysis of up to 35 samples, derived from cells, tissues, or biological fluids can be performed with accuracy and precision.
The amine-reactive TMTpro reagents are used for labeling tryptic peptides irrespective of their protein sequence or enzyme specificity. The isobaric TMTpro label reagents are similar in design to the original TMT label reagents except that they have a longer spacer region and isobutyl proline mass reporter region (Figure 1). After MS/MS fragmentation, each TMTpro tag generates a unique reporter mass (126–135 Da) in the low-mass region of the high-resolution MS/MS spectrum that is used for relative protein quantitation.
The workflow for TMTpro and original TMT label reagents is identical (Figure 2). Additional mass spectrometry reagents such as standards, buffers, antibody and sample prep kit can be purchased separately (Table 2).
TMTpro zero label reagent (serves as a ‘light’ tag) is used for method development and labelling optimization before multiplexed analysis of labeled samples. It is a suitable alternative to costly isotope-labeled compounds. The TMTpro zero label reagent allows testing and optimization of sample preparation, labeling, fractionation, and fragmentation for peptide identification and reporter detection.
Figure 1. Structural design of TMTpro label reagents. The functional regions of the TMTpro reagents’ structure including MS/MS fragmentation site by higher-energy collision dissociation (HCD).
Figure 2. Schematic summary for MS experiments using TMTpro or TMT original isobaric mass tagging reagents. Protein extracts isolated from cells or tissues are reduced, alkylated, and then digested using the EasyPep Mini MS Sample Prep kit or an equivalent method [2]. Samples are then labeled with the TMT or TMTpro reagents before sample mixing, fractionation, and cleanup. Labeled samples are analyzed on a high-resolution Orbitrap LC-MS/MS mass spectrometer before data analysis to identify peptides and quantify relative abundance of reporter ions.
Workflow step | Product | Unit size | Cat. No. |
---|---|---|---|
TMT standard | Pierce TMT11plex Yeast Digest Standard | 20 µg | A40938 |
5 x 20 µg | A40939 | ||
TMT labeling | 1M Triethylammonium bicarbonate (TEAB) for TMT experiments | 50 mL | 90114 |
TMT quench | 50% Hydroxylamine for TMT experiments | 5 mL | 90115 |
Enrichment of TMT-labeled peptides | TMT Monoclonal Antibody (25D5) | 100 µg | 90075 |
Enrichment of TMT-labeled peptides | Immobilized Anti-TMT Antibody Resin | 6 mL | 90076 |
Enrichment of TMT-labeled peptides | TMT Elution Buffer | 20 mL | 90104 |
TMT-compatible peptide sample prep | EasyPep MS Sample Prep Kit | 8 reactions | A45734 |
20 reactions | A40006 | ||
96 reactions | A45733 | ||
TMT-compatible peptide sample prep | EasyPep Lysis Buffer | 100 mL | A45735 |
TMT label reagents (TMT tags) enable multiplex (2-plex to 11-plex) relative quantitation of biological samples using protein mass spectrometry. The chemical structure of TMT reagents consists of a peptide-reactive labeling group, a mass reporter, and a spacer arm (mass normalizer) (Figure 3). Based on the reactive chemistry, the TMT reagents are classified into three categories—amine reactive, cysteine-reactive, and carbonyl-reactive.
The TMT 10-plex and 11-plex label reagents share an identical structure with TMTzero, TMTduplex, and TMTsixplex reagents, but contain different numbers and combinations of 13C and 15N isotopes in the mass reporter.
Figure 3. Structural design of TMT reagents. The functional regions of the TMT reagents’ structure including MS/MS fragmentation site by higher-energy collision dissociation (HCD) and electron transfer dissociation (ETD) and collision induced dissociation (CID). Amine-reactive, cysteine-reactive, and carbonyl-reactive TMT reagents are shown in (A), (B), and (C).
TMT labeling reagents in lyophilized format are extremely reactive to moisture and great handling care must be taken to ensure proper functionality. A subset of TMT 10- and 11-plex and TMTpro 16- and 18-plex label reagents have been developed to be stable in liquid format and are pre-aliquoted in 96-well plates for simplified processing.
Highlights include:
Figure 4. TMT labeling reagent in 96-well plate. Flexible format of 96-well TMT labeling reagent in stable solution with barcoding for optional automation use, with an easy to peel or puncture foil seal. For partial plate use, TMT and TMTpro labeling reagents can be thawed and refrozen multiple times with continued high performance. (A) TMT and TMTpro in stabilized solution pre-aliquoted in 96-well plate. (B) Foil seal can be peeled and cut for partial plate use. (C) Foil seal can easily be punctured permitting partial plate use.
The Super heavy TMT (shTMT) and shTMTpro label reagents are non-isobaric amine-reactive TMT variants and are a suitable alternative for precursor-based protein quantification [3, 4]. These TMT derivatives contain twice the number of heavy isotopes (+11 mass units) that are dispersed throughout the reporter and spacer regions (Figure 4). They are used as a mass offset for triggering peptide selection. The workflow for Super heavy TMT (shTMT) and shTMTpro label reagents is identical (Figure 5). The shTMT label reagent is used in combination with TMT- or TMTpro-labeled samples and the mass offset from the super heavy-labeled synthetic peptide serves as a trigger for the selection of the targeted peptide in the multiplexed sample. This triggered selection reduces interference/co-isolation to enable more robust quantitation.
Figure 5. Chemical structure of Super Heavy TMTpro (shTMTpro) label reagents.
Figure 6. Super Heavy TMTpro workflow.
The AccelerOme reagents are the newest additions to the next generation tandem mass tags (TMT) to support automated sample preparation and TMT labeling on the AccelerOme Automated Sample Preparation Platform. The integrated instrument and reagent platform enables automated sample handling of up to 36 samples for the reduction, alkylation, and digestion of proteins, followed by TMT or TMTpro labeling and clean-up. The TMT or TMTpro-labeled samples are fit-for-purpose for analysis using high resolution Thermo Scientific Orbitrap LC-MS/MS instruments (Figure 6).
Figure 7. Supporting kits and workflow of AccelerOme automated sample preparation system.
Learn how to prepare and label peptide samples with tandem mass tags for quantitative proteomics analysis.
Explore our webinars around protein quantitation. Click on the discovery proteomics section to find this webinar that discusses the advantages of sample multiplexing and reviews isobaric tag workflows. New TMT chemistry, applications, and integration of mass spectrometry sample preparation with quantitative workflows will be introduced.
For Research Use Only. Not for use in diagnostic procedures.