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Vector-Based RNAi
Expand your experimental possibilities beyond synthetic siRNAs using vector-based expression of target-specific shRNA and miRNA. Achieve:
- Stable or transient target knockdown
- Lentiviral delivery in hard-to-transfect, primary, and non-dividing cells
- Co-expression of a fluorescent reporter for visualization
RNAi vector technologies allow you to:
- Regulate gene inhibition with inducible RNAi expression
- Select for a pure population of cells stably expressing an RNAi sequence
- Control gene expression in vivo with tissue-specific promoters
Summary of Invitrogen RNAi vector technologies
| BLOCK-iT Pol II miR RNAi RECOMMENDED Learn more | BLOCK-iT shRNA Learn more | |
|---|---|---|
| Description | microRNA-adapted inserts for more efficient processing of the RNAi molecule | Traditional short hairpin inserts for transient or long-term RNAi |
| Promoter type | Pol II | Pol III |
| # shRNA per vector | Polycistronic expression of miR-adapted shRNAs (chaining) | Single shRNA expression per promoter |
| Expression tracking | Co-expression of GFP allows expression to be tracked | Delivery can be tracked with a separate cassette but not by shRNA expression |
| Vector compatibility | Compatible with most Gateway destination vectors | Compatible with BLOCK-iT destination vectors |
| Products | Pre-designed and custom miR RNAi vector inserts
| Custom shRNA vectors for any organism Lentiviral and adenoviral options available |
Highly efficient delivery of BLOCK-iT Pol II miR RNAi Expression Vectors or BLOCK-iT shRNA Vectors is necessary to achieve significant levels of knockdown. Optimizing transfection or transduction, controlling for experimental variability, and using a powerful transfection reagent vastly improves the chances for RNAi success. The use of positive and negative controls will also help in the assessment of your experiments.
Optimizing delivery conditions
- Start with the cell-type specific protocol and transfection reagent most suitable for your experiment
- Use a DNA plasmid that expresses a fluorescent protein, such as GFP, to monitor that the plasmid has entered the cell
- Apply a viability indicator such as Ethidium Homodimer-1 (EthD-1) to monitor cell viability related to transfection conditions
- Utilize Hoechst nuclei stain to measure the percent of transfected and/or dead cells to the total cell population
Resources
Questions?
Technical inquires:
Our Technical Application Scientists are available to help assist you at techsupport@thermofisher.com
Ordering & Order Status inquires:
If you have questions about pre-designed RNAi orders and order status, please contact us at genomicorders@thermofisher.com
If you have any questions about Custom RNAi orders and order status, please contact us at RNAiSupport@thermofisher.com
For Research Use Only. Not for use in diagnostic procedures.