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Mass Spectrometry Sample Quantitation Support
Explore our “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems. Browse through our "Guides and Tools" section to access a comprehensive portfolio of product-related support resources.
Getting Started
- What is the difference between Relative and Absolute Quantitation? Which type of quantitation is used for proteomic samples?
- What is the main purpose of Tandem Mass Tag (TMT) reagents?
- What type of mass spectrometer can I use for Tandem Mass Tag (TMT) analysis?
- How many samples can I combine for simultaneous, multiplex proteomic mass spec quantitation?
- What sample types can I use with different mass spec quantitation systems?
Troubleshooting
- I have a lower number of peptide/protein identification in my combined TMT-labeled samples compared to the unlabeled sample. What can I do?
- I have low levels (<95%) of TMT-labeling for my samples. What should I check?
- I have low incorporation of heavy amino acids in my SILAC proteins. What should I check?
- What software can I use for protein and peptide quantitation?
- I have good peptide identification numbers but the variation between sample replicates is high. What do you recommend to improve sample reproducibility?
Guides and Tools
- Application Note: Relative Quantitation of TMT-Labeled Proteomes – Focus on Sensitivity and Precision
- Application Note: Quantitation of TMT-Labeled Peptides Using Higher-Energy Collisional Dissociation on the Velos Pro Ion Trap Mass Spectrometer
- Application Note: Relative Quantitation of Protein Digests Using Tandem Mass Tags and Pulsed-Q Dissociation (PQD)
- Application Note: Time-resolved Analysis of Proteome Dynamics by Tandem Mass Tags and Stable Isotope Labeling in Cell Culture (TMT-SILAC) Hyperplexing
- Poster: The Power of Quantitative Multiplexing
- Tech Tip : Strategies for Removal of Unreacted TMT Label Reagent
- Tech Tip : TMT Data Acquisition on the LTQ-Orbitrap XL Mass Spectrometer
- Overview: Workflow for Protein Mass Spectrometry
- Overview: Protein Quantitation Using Mass Spectrometry
- Overview: Quantitative Proteomics
- Overview: Relative versus Absolute Quantitation
- Overview: TMT Systems
- Overview: SILAC Metabolic Labeling Systems
- Overview: SureQuant Targeted Mass Spec Assay Kits
- Application Note: Specific Labeling, Enrichment and Quantitation of S-Nitrosylated Peptides Using IodoTMT Reagents
- Application Note: Quantitative Cancer Stem Cell Phosphoprotein Profiling
- Application Note: Develop Mid-to High-throughput SRM and MRM Assays
- Handbook: Thermo Scientific Pierce Reagents for Quantitative Proteomics
- DailyCalcs Science Calculator: An app designed to calculate molarity, dilution, molecular weight, and more. Also includes a transfection calculator you can use to simplify transfection experimental setup.
Custom Services
Custom Peptide Synthesis Services
Thermo Scientific Custom Peptide synthesis service offers numerous options for synthesis platforms, purity levels, modifications, and formats to meet a variety of research needs. We synthesize peptides of 2–110 amino acids using the latest FMOC solid-phase technology, and purify our products using HPLC. Synthesis scales range from 0.1 mg to 1 g, and purities from crude to >98% based on the service.
HeavyPeptide AQUA Custom Synthesis Service
Thermo Scientific HeavyPeptide AQUA Custom Synthesis Service provides isotopically labeled, AQUA-grade peptides for the relative and absolute quantitation of proteins at very low concentrations in complex protein mixtures. Peptides are synthesized using the latest FMOC solid-phase technology and purified by HPLC. All HeavyPeptide sequences come with mass spectrometric analysis and analytical HPLC dat. Additionally, we offer a wide range of labels, modifications, scales, and purities.
Bulk and Custom Protein Biology Products
Partner with us to scale up your process or product development pipeline with bulk quantities of high-quality immunoassay reagents and biochemicals. We offer a variety of compounds, devices, and resins for your large-scale applications. Whether you are developing an assay for commercialization, constructing a large screening assay for in-house use, or requiring high-purity reagents for your manufacturing process, we can provide consistent quality in milligram to kilogram quantities manufactured under ISO 9001 processes. Complete our bulk and custom products request form to get your project started.
For Research Use Only. Not for use in diagnostic procedures.
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