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High-Content Analysis (Quantitative Imaging) Information
High-content analysis, also called quantitative imaging, is a process where automated microscopy is combined with multi-parametric imaging. Visualization software provides quantitative data about cell populations. In this section you will find application notes, video tutorials, question and answer videos as well as webinars all designed to help you better understand what high-content analysis is all about and how to get the most out of your data.
High-content analysis (quantitative imaging) features
BioProbes article
HCA platforms for automated, quantitative analyses
A wealth of information about a physiological process or pathological condition can be collected by monitoring the localization and abundance of specific proteins using fluorescence imaging but quantifying these essential processes is time consuming and laborious. High-content analysis can remove these obstacles, resulting in robust and reproducible research.
On-demand webinar
Breathing new life into hypoxia research
In this webinar, we will discuss hypoxia in human diseases, classical methods for setting up hypoxic conditions, and novel instruments and reagents for imaging cells in hypoxic conditions.
High-content analysis (quantitative imaging) learning resources
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| Type | Title | Categories |
|---|---|---|
| Application note (2012) | Monitoring neurite morphology and synapse formation in primary neurons for neurotoxicity assessments and drug screening | ArrayScan, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, neuroscience, viability |
| Application note (2013) | Optimizing the high content analysis of cell morphology using multi-dimensional imaging on the Thermo Scientific ArrayScan XTI HCA Reader: Stem cell colonies | Alexa Fluor, antibodies, ArrayScan, confocal microscopy, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, immunofluorescence (IF), microplate reader, particles, stem cell research |
| Application note (2013) | Multiplexed mitosis and apoptosis analysis | antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, particles |
| Application note (2014) | Using a high content analysis database as a repository for data | database management, drug discovery, high content analysis, microplate reader |
| Application note (2014) | High content cell cycle screening: Pairing FUCCI technology with Thermo Scientific HCS Studio 2.0 Software and FCS Express Image cytometry | ArrayScan, cancer, cell cycle, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader |
| Application note (2014) | Oxidative stress measurements made easy: CellROX reagents with simple workflow for high content imaging | ArrayScan, CellInsight, CellROX, high content analysis, oxidative stress |
| Application note (2014) | Elucidating the mechanism of cell death through the use of high content analysis | apoptosis, autophagy, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis |
| Application note (2015) | Quantitation of proliferating cells with the EVOS FL Auto Imaging System | antibodies, antibody labeling, ArrayScan, cell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, onstage incubator, phagocytosis |
| BioProbes articles (Issues 50– present day) | BioProbes Journal of Cell Biology Application | cell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification |
| Protocol | Pharmacological induction of apoptosis with camptothecin | cell vitality, flow cytometry, high content analysis, imaging, microplate |
| Protocol | Hoechst 33342 for HCA instruments | cell structure, high content analysis |
| Protocol | HCS NuclearMask Red Stain | cell structure, high content analysis |
| Protocol | HCS NuclearMask Deep Red Stain | cell structure, high content analysis |
| Protocol | HCS NuclearMask Blue Stain | cell structure, high content analysis |
| Protocol | HCS Mitochondrial Health Kit | cell viability, high content analysis |
| Protocol | HCS LIVE/DEAD Green Kit using Hoechst 33342 | cell viability, high content analysis |
| Protocol | HCS LIVE/DEAD Green Kit using HCS NuclearMask Deep Red | cell viability, high content analysis |
| Protocol | HCS CellMask Stains | cell structure, high content analysis |
| Protocol | DAPI for HCA instruments | cell structure, high content analysis |
| Protocol | Click-iT EdU HCS assays | cell proliferation, imaging |
| Scientific poster (2006) | LipidTOX dyes for adipocyte staining in routine imaging applications | cell differentiation, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, lipid detection, lipids, stem cell research, viability |
| Scientific poster (2006) | Cell cycle analysis using microplate cytometry: A comparison of laser and dye combinations | Acumen Explorer, cell cycle, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader |
| Scientific poster (2006) | New fluorescent reagents for the imaging-based assays for the analysis of drug-induced perturbations of cellular lipid metabolism | ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, lipid detection, lipids, microplate reader, viability |
| Scientific poster (2006) | Utility of 405 nm-excitable dyes in high content screening using an Acumen Explorer Microplate Cytometer | Acumen Explorer, apoptosis, cell cycle, cell health, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, violet laser-excited reagents |
| Scientific poster (2007) | Evaluation of click chemistry-based alternative to BrdU antibody labeling in tissue and cultured cells using fluorescence microscopy and flow cytometry | antibodies, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, microplate reader, nucleic acid labeling |
| Scientific poster (2007) | A new approach for the detection of intracellular glutathione by fluorescence microscopy and flow cytometry | ArrayScan, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, live-cell imaging, microplate reader, viability, violet laser-excited reagents |
| Scientific poster (2007) | Click chemistry-based detection of S-phase adherent cells using automated microscopy and image analysis | Alexa Fluor, antibodies, ArrayScan, cell cycle, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader |
| Scientific poster (2007) | Click catalyzed nucleic acid labeling as a novel replacement for BrdU antibody-based cell proliferation assay | Alexa Fluor, antibodies, antibody labeling, cell cycle, cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling |
| Scientific poster (2007) | BacMam gene delivery of targeted fluorescent proteins, Organelle Lights for HCS | BacMam technology, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, live-cell imaging, nanocrystals, Qdot |
| Scientific poster (2008) | Quantum dot (Qdot) labeling of gene expression in fresh frozen brain tissue using high-throughput in situ hybridization | in situ hybridization, brightfield microscopy, confocal microscopy,fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, high content analysis, nanocrystals, neuroscience, Qdot, tissue |
| Scientific poster (2008) | Characterization of DNA content, cyclin B1 and phosphorylated histone H3 with direct S-phase using EDU incorporation in multiparameter testing of cell lines with cell cycle blocking agents | Alexa Fluor, antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis |
| Scientific poster (2008) | Cell-based potassium ion channel screening with the FluxOR Assay | BacMam technology, cell counting, Countess, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader |
| Scientific poster (2008) | Novel Click-IT TUNEL Assay for detection of cell death | Alexa Fluor, antibodies, apoptosis, Click-iT, cytotoxicity, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, high content analysis, immunocytochemistry (ICC), viability |
| Scientific poster (2008) | Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry | Alexa Fluor, antibodies, ArrayScan, cell cycle, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling, nucleic acid quantitation |
| Scientific poster (2008) | FluxOR, a universal potassium channel assay: Screening BacMam-delivered and stably expressed targets | BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader |
| Scientific poster (2008) | ThiolTracker Violet dye, a new 405 nm excitable fluorescent probe for detecting intracellular thiols in imaging-based assays | ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, glutathione, high content analysis, microplate reader, protein labeling, violet laser-excited reagents |
| Scientific poster (2008) | A new 405 nm excitable fluorescent probe of intracellular thiols for imaging and flow cytometric assays | ArrayScan, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, high content analysis, microplate reader, thiol modification, violet laser-excited reagents |
| Scientific poster (2008) | Detection of DNA synthesis by automated microscopy and image analysis: Comparison of BRDU method and a new click chemistry-based EDU method | Alexa Fluor, antibodies, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling |
| Scientific poster (2009) | Cell-based assays for predictive hepatotoxicity measurements using high content imaging | Alexa Fluor, ArrayScan, cell structure-plasma membrane, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, high content analysis, microplate reader, nucleic acid quantitation, viability |
| Scientific poster (2009) | A non-radioactive method for detecting nascent protein synthesis by high-content imaging utilizing click chemistry | Alexa Fluor, apoptosis, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, protein detection |
| Scientific poster (2009) | Monitoring mitotic cells and DNA content by automated imaging and analysis | Alexa Fluor, cell cycle, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling, nucleic acid quantitation, viability |
| Scientific poster (2009) | High content imaging of endocytosis & phagocytosis using pHrodo conjugates | Alexa Fluor, ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, pH detection, phagocytosis, viability |
| Scientific poster (2009) | Click chemistry-based detection of nascent RNA synthesis using high content imaging and fluorescence microscopy | Alexa Fluor, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling, viability |
| Scientific poster (2009) | High content analysis of cytotoxicity by laser scanning fluorescence microplate cytometry | Alexa Fluor, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, viability |
| Scientific poster (2009) | High content imaging and analysis of mitotoxicity and cytotoxicity in fixed cells | cell structure-mitochondria, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, viability |
| Scientific poster (2009) | Quantitative analysis of genotoxicity and cytotoxicity to DNA damaging agents using high-content imaging | Alexa Fluor, antibodies, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling, nucleic acid quantitation, viability |
| Scientific poster (2009) | Cell-based potassium ion channel screening with BacMam using the FluxOR Assay | BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader |
| Scientific poster (2009) | Click chemistry-based assays for fluorescence microscopy and high content imaging | Alexa Fluor, apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid detection, protein detection, viability |
| Scientific poster (2010) | High content imaging of autophagy | antibodies, ArrayScan, autophagy, BacMam technology, cell signaling, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader |
| Scientific poster (2010) | Oxidative stress-induced protein modification: Application of clickable linoleic acid analogs | Alexa Fluor, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, high content analysis, microplate reader, oxidative stress, protein detection, protein enrichment |
| Scientific poster (2010) | Antibody- and fluorescent protein-based approaches to measuring autophagy in mammalian cells by fluorescence microscopy | Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging |
| Scientific poster (2010) | Novel tools to enable high resolution gene expression by investigating the nascent transcriptome | Alexa Fluor, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, high content analysis, magnetic beads, nucleic acid labeling, PCR/qPCR, viability |
| Scientific poster (2010) | Autophagosomal accumulation perturbs Golgi structure without affecting other organelles: Implications for autophagosome biogenesis | Alexa Fluor, antibodies, autophagy, BacMam technology, cell structure-golgi, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging |
| Scientific poster (2010) | A new generation of cellular imaging probes for neurosciences research | autophagy, BacMam technology, CellLight, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels, ion indicators |
| Scientific poster (2010) | NA-Fluor Influenza Neuraminidase Assay Kit: Standardization of the classic MUNANA-based neuraminidase activity assay for monitoring antiviral resistance in influenza | drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, influenza, microbiology, microplate reader |
| Scientific poster (2010) | Simultaneous analysis of cell death mechanisms and oxidative stress using live cell fluorescence microscopy | apoptosis, ArrayScan, BacMam technology, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader, oxidative stress |
| Scientific poster (2010) | The NA-XTD Influenza Neuraminidase Assay Kit: Extended-glow chemiluminescence assay system for highly sensitive influenza neuraminidase assays | chemiluminescence, drug discovery,fluorescence microscopy/fluorescence imaging, high content analysis, influenza, microbiology, microplate reader |
| Scientific poster (2010) | BacMam gene expression for functional and imaging applications | BacMam technology, cell cycle, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels |
| Scientific poster (2011) | Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health | ArrayScan, cell health, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress, viability |
| Scientific poster (2011) | Simultaneous analysis of cell death mechanisms and oxidative stress using high content imaging | Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress |
| Scientific poster (2011) | Primary human cells from Life Technologies: Offerings, scale and applications in HCS and HTS formats | Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, cell signaling, Click-iT, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis |
| Scientific poster (2011) | Validation of high content imaging and analysis assays for cell health and cytotoxicity | antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, cell health, cell proliferation, CellLight, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, pH detection |
| Scientific poster (2011) | The next generation of cell-based imaging assays for apoptosis, autophagy and oxidative stress from Molecular Probes | Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress |
| Scientific poster (2011) | NA-Fluor and NA-XTD Influenza Neuraminidase Assays for neuraminidase quantitation and inhibition assays | chemiluminescence, drug discovery,fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, influenza, microbiology, microplate reader |
| Scientific poster (2012) | Cell-based analysis of oxidative stress, lipid peroxidation and lipid peroxidation-derived protein modifications using fluorescence microscopy | flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, high content analysis, lipid peroxidation, microplate reader, oxidative stress, viability |
| Scientific poster (2013) | Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health | apoptosis, ArrayScan, caspase substrates, cell health, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, microplate reader, oxidative stress |
| Scientific poster (2013) | Illuminating endocytosis with targeted pH-sensitive fluorescent compounds | Alexa Fluor, ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, pH detection, phagocytosis, viability |
| Scientific poster (2014) | New fluorescent probes and sensors for visualizing endocytosis, lysosomal dynamics and autophagy | autophagy, cell structure-lysosomes, endocytosis/phagocytosis, autophagy, fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader |
| Scientific poster (2015) | Visualizing the life and death of cells: novel probes and platforms | apoptosis, ArrayScan, autophagy, cell death, CellInsight, fluorescence microscopy/fluorescence imaging, high content analysis |
| Scientific poster (2016) | High content screening in MCF7 and MDA-MB231 cells show differential responses depending on oxygen levels and mechanistic readout for viability | cell health, cell viability and function, drug discovery, high content analysis, high throughput screening |
| Scientific poster (2016) | Incorporating histological analysis into existing high content imaging | antibodies, ArrayScan, brightfield microscopy, fluorescence microscopy/fluorescence imaging, high content analysis, immunohistochemistry (IHC) |
| Scientific poster (2017) | Hypoxia measurements in live and fixed cells using fluorescence microscopy and high-content imaging | EVOS, fluorescence microscopy/fluorescence imaging, high content analysis, hypoxia, live-cell imaging, onstage incubator |
| Tutorial | HCA Q & A A video series that answers common questions about high content analysis and the different Thermo Scientific high content imaging platforms | ArrayScan, CellInsight, high content analysis |
| Webinar | Basic techniques in autophagy research This webinar will introduce you to a series of analytical tools and techniques to help you identify and interrogate key features of autophagy. Topics to be covered include: Tips and tricks for selecting the right tools and achieving the best results Fluorescent proteins and antibodies used to analyze both live and fixed cells Analysis steps using a variety of multiplexing options, with quantitative methods for image analysis or fluorescence intensity measurement | autophagy, cell health, fluorescence microscopy/fluorescence imaging, fluorescent proteins, high content analysis, immunofluorescence (IF) |
| Webinar | Breathing new life into hypoxia research Although the significance of hypoxia in biological processes is well known, creating model systems with accurate control of hypoxic conditions is extremely difficult without access to elaborate systems that allow precise control and maintenance of temperature, humidity, and gases (CO2 and O2) during an experiment. In this webinar, we will discuss: Overview of hypoxia in human diseases Classical methods for setting up hypoxic conditions Novel instruments and reagents for imaging cells in hypoxic conditions | EVOS, fluorescence microscopy/fluorescence imaging, high content analysis, hypoxia, onstage incubator |
For Research Use Only. Not for use in diagnostic procedures.