General Transfection Protocols

Our Lipofectamine Reagent Protocols have been optimized for efficiency, viability, and reproducibility across a broad range of cell types. This is often the best place to start, especially in a new cell line. If you find this doesn’t work for your specific cell type, then you can you look to our cell-specific protocols for further optimization.

Cell-type Specific Transfection Protocols

* With the exception of CHO-K1, HeLa-S3, HEK 293, and MCF-7 cells, We found that the use of Plus Reagent (Cat# 11514-015) together with Lipofectamine LTX further enhanced transfection in the rest of the cell lines.