Wet Tank Transfer Systems

• 2-in-1 SureLock Tandem Midi Gel Tank—no need to purchase a separate tank for western transfers
• Room temperature transfer—save the time, planning, and mess required with other systems to pre-chill buffers, freeze ice packs, or prepare ice baths
• 300 mL per gel transfer buffer volume—lower transfer buffer volume requirement reduces reagent consumption and hazardous disposal costs
• Fast and efficient—30-minute transfer without compromising performance
• Easy to use—convenient blot assembly instructions are printed on the accessory transfer tray
• Robust electrode plates—for highly efficient and reliable western transfers

When used with high-performance Invitrogen precast midi gels, you can depend on the SureLock Tandem Midi Gel Tank and Midi Blot Module to generate consistent, high-throughput western blots. The Invitrogen midi gels offer reproducible high-performance protein separation, resulting in sharp, well-resolved bands in straight lanes, and the SureLock Tandem Midi Blot Module provides dependable uniform transfer of proteins across the gel.

To demonstrate the SureLock Tandem Midi Blot Module’s uniformity of transfer across a membrane, a NuPAGE 4-12% Bis-Tris Midi gel was loaded with recurring dilutions of E. coli lysate ranging from 2-0.25 µg and SeeBlue Plus2 Pre-stained Protein Standard. Electrophoresis was performed and the gel was transferred onto a 0.45 µm PVDF membrane. Results are shown in Figure 1.

Figure 1. Uniform transfer using SureLock Tandem Midi Gel Tank. Total protein normalization of transferred proteins shows uniform transfer across the blot. 

A. No-Stain Reagent Labeled PVDF Blot. A NuPAGE 4-12% Bis-Tris Midi gel was loaded with recurring dilutions of E. coli Lysate ranging from 2 to 0.25 µg and SeeBlue Plus2 Pre-stained Protein Standard in lanes 1-2 and 19-20. The gel was electrophoresed using MES running buffer in the SureLock Tandem Midi Gel Tank. Proteins from the gel were transferred onto a 0.45 µm PVDF membranes at 25V constant for 30 minutes using the SureLock Tandem Midi Blot Module. After transfer, the proteins on the membrane were labeled following the No-Stain Protein Labeling Reagent protocol for midi-sized membranes. The image was captured using an iBright Imager with the No-Stain Membrane epi setting (455-485 nm excitation and 565-615 nm emission). As the SeeBlue Plus2 Pre-stained Protein Standard is already labeled, its protein bands are not all susceptible to labeling with No-Stain Reagent, and therefore are not all visible in this image. 

B. Densitometric Signal Linearity versus Protein Load. The densitometric signal intensity was determined for each lane. Technical replicates (n=4) were averaged and plotted to determine the linear regression for the entire concentration range (R2=0.9994). Error bars represent standard deviation.