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Table 8.7 Amine-reactive dyes for nucleic acid sequencing.

Cat. No.Reactive Dye *Ex/Em §Handbook Location
C22105-FAM, SE †494/518Fluorescein, Oregon Green and Rhodamine Green Dyes—Section 1.5
C61646-FAM, SE494/518Fluorescein, Oregon Green and Rhodamine Green Dyes—Section 1.5
C61275-CR 6G, SE525/555Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6
C6171MP6-JOE, SE †522/550Fluorescein, Oregon Green and Rhodamine Green Dyes—Section 1.5
C22115-TAMRA, SE555/580Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6
C61236-TAMRA, SE †555/580Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6
C61255-ROX, SE580/605Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6
C61266-ROX, SE †580/605Long-Wavelength Rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6
D2184BODIPY FL, SE ‡505/513BODIPY Dye Series—Section 1.4
D6140BODIPY FL, SSE505/513BODIPY Dye Series—Section 1.4
D6102BODIPY FL-X, SE505/513BODIPY Dye Series—Section 1.4
D6180BODIPY R6G, SE ‡528/550BODIPY Dye Series—Section 1.4
D6117BODIPY TMR-X, SE542/574BODIPY Dye Series—Section 1.4
D2222BODIPY 564/570, SE ‡565/571BODIPY Dye Series—Section 1.4
D2228BODIPY 581/591, SE ‡584/592BODIPY Dye Series—Section 1.4
D6116BODIPY TR-X, SE589/617BODIPY Dye Series—Section 1.4
* FAM = carboxyfluorescein; CR 6G = carboxyrhodamine 6G; JOE = 6-carboxy-4',5'-dichloro-2',7'-dimethoxyfluorescein; TAMRA = carboxytetramethylrhodamine; ROX = carboxy-X-rhodamine; BODIPY = a substituted 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene derivative; SE = succinimidyl ester; SSE = water-soluble sulfosuccinimidyl ester. † These are the most widely used isomers for DNA sequencing.ref ‡ These BODIPY derivatives were reported to be useful for automated DNA sequencing, in part because the dyes have a minimal effect on the mobility of the fragment during electrophoresis and also exhibit well-resolved spectra with narrow bandwidths.ref § Excitation (Ex) and emission (Em) maxima in nm.

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