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This kit includes Image-iT DEAD Green viability stain for identification of dead cells. After staining your cells, the signal from the stain will be retained if the sample is fixed and permeabilized.
1. Culture cells in appropriate medium and vessel for microscopy |
2. Dilute Image-iT DEAD Green stain by adding 225 µL DMSO to vial to make a 1,000X stock solution |
3. Add 1 µL stock solution per milliliter of medium to label cells |
4. Incubate 30 minutes at 37°C |
5. Image cells |
Image-iT DEAD Green | |
---|---|
Excitation/Emission | 488/515 nm |
Standard filter set | FITC or GFP |
EVOS Light Cube | GFP |
Storage conditions | Room temperature |
Valinomycin-treated HeLa cells labeled with the Image-iT DEAD Green Viability Stain.