miRNA Analysis

MicroRNAs (miRNAs) are short, highly conserved small noncoding RNA molecules naturally occurring in the genomes of plants and animals. They are 17–27 nucleotides long and regulate posttranscriptional mRNA expression, typically by binding to the 3’ untranslated region (3’-UTR) of the complementary mRNA sequence, resulting in translational repression and gene silencing. Studies have shown that thousands of human protein-coding genes are regulated by miRNAs, indicating that miRNAs are “master regulators” of many important biological processes.

RNAs that mimic endogenous miRNAs and enable miRNA functional analysis by up-regulation of miRNA activity or that bind to and inhibit endogenous miRNA molecules and enable miRNA functional analysis by down-regulation of miRNA activity.

Quantitate miRNAs with the specificity and sensitivity of TaqMan Assay chemistry. A simple, two-step protocol requires only reverse transcription with a miRNA-specific primer, followed by real-time PCR with TaqMan probes.

GeneChip™ miRNA Arrays are powerful tools for studying the role of small non-coding RNA (miRNA, snoRNA, and scaRNA) in complex diseases, such as cancer.

QuantiGene™ miRNA Singleplex Assays are 96-well, hybridization-based assays that utilize a branched DNA technology for signal amplification for the direct quantitation of target miRNAs.