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Compatible reagents
iBright Imaging Systems offer up to five imaging modes to support your applications. Efficiently and easily capture data from protein gels, nucleic acid gels, chemiluminescent western blots, fluorescent western blots, and more. Get the most from your investment by having the freedom to choose from multiple detection strategies to meet your experimental needs.
| Image mode | What kind of signal can be captured? |
Protein gel |
Colorimetric staining of gels (e.g., Coomassie, silver) and membranes (e.g., Ponceau S, Thermo Scientific Pierce Reversible Protein Stain), fluorescent staining of gels (e.g., Invitrogen SYPRO Ruby stain) |
Nucleic acid gel |
Ethidium bromide and a variety of fluorescent nucleic acid stains (e.g. Invitrogen SYBR stains) |
Chemiluminescent blot |
Chemiluminescence using all popular HRP and AP substrates (e.g., Thermo Scientific SuperSignal and Invitrogen WesternBreeze substrates) |
Fluorescent blot |
Fluorescence with popular RGB (visible range) and near-IR fluorophores (e.g., Invitrogen Alexa Fluor and Alexa Fluor Plus conjugates) |
Universal |
Custom mode to image samples containing multiple signals, such as chemiluminescence, fluorescence, colorimetric stains, and/or visible images; image display is similar to fluorescent blot mode and allows one to assign false color to any sample |
Fluorescent western blot imaging
With the five fluorescent channels of the iBright FL1500 Imaging System, one can image with many different fluorophore options. Up to 4-channel multiplexing is possible, utilizing fluorophores in both the visible (RGB) fluorescent range and the near-infrared (near-IR) range. The table below shows available fluorescence channels on iBright Imaging Systems and some examples of fluorophores that can be detected in each.
Fluorescence channels on the iBright FL1500 Imaging System
Excitation channel |
Filter range (nm) |
Emission channel |
Filter range (nm) |
EX1 |
455-485 |
EM1 |
508-557 |
EX2 |
515-545 |
EM2 |
568-617 |
EX3 |
608-632 |
EM3 |
675-720 |
EX4 |
610-660 |
EM4 |
710-730 |
EX5 |
745-765 |
EM5 |
800-850 |
Fluorescence channels on the iBright FL1500 Imaging System
Excitation channel |
Emission channel |
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Alexa Fluor 488, Alexa Fluor Plus 488 |
455-485 nm (EX1) |
508-557 nm (EM1) |
Alexa Fluor 546 |
515-545 nm (EX2) |
568-617 nm (EM2) |
Alexa Fluor 555, Alexa Fluor Plus 555 |
515-545 nm (EX2) |
568-617 nm (EM2) |
Alexa Fluor 568 |
515-545 nm (EX2) |
568-617 nm (EM2) |
Alexa Fluor 594 |
608-632 nm (EX3) |
675-720 nm (EM3) |
Alexa Fluor 633 |
608-632 nm (EX3) |
675-720 nm (EM3) |
Alexa Fluor 647, Alexa Fluor Plus 647 |
608-632 nm (EX3) |
675-720 nm (EM3) |
Alexa Fluor 660 |
610-660 nm (EX4) |
675-720 nm (EM3) |
Alexa Fluor 680, Alexa Fluor Plus 680 |
610-660 nm (EX4) |
710-730 nm (EM4) |
Alexa Fluor 750 |
745-765 nm (EX5) |
800-850 nm (EM5) |
Alexa Fluor 790 |
745-765 nm (EX5) |
800-850 nm (EM5) |
Alexa Fluor Plus 800 |
745-765 nm (EX5) |
800-850 nm (EM5) |
Excitation channel |
Emission channel |
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DyLight 488 |
455-485 nm (EX1) |
508-557 nm (EM1) |
DyLight 549/550 |
515-545 nm (EX2) |
568-617 nm (EM2) |
DyLight 594 |
608-632 nm (EX3) |
675-720 nm (EM3) |
DyLight 633 |
608-632 nm (EX3) |
675-720 nm (EM3) |
DyLight 649/650 |
608-632 nm (EX3) |
675-720 nm (EM3) |
DyLight 680 |
610-660 nm (EX4) |
710-730 nm (EM4) |
DyLight 755 |
745-765 nm (EX5) |
800-850 nm (EM5) |
DyLight 800 |
745-765 nm (EX5) |
800-850 nm (EM5) |
Excitation channel |
Emission channel |
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Qdots 525 |
455-485 nm (EX1) |
508-557 nm (EM1) |
Qdots 565 |
455-485 nm (EX1) |
568-617 nm (EM2) |
Qdots 585 |
455-485 nm (EX1) |
568-617 nm (EM2) |
Qdots 605 |
455-485 nm (EX1) |
568-617 nm (EM2) |
Qdots 625 |
455-485 nm (EX1) |
568-617 nm (EM2) |
Qdots 655 |
455-485 nm (EX1) |
675-720 nm (EM3) |
Qdots 705 |
455-485 nm (EX1) |
675-720 nm (EM3) |
Qdots 800 |
455-485 nm (EX1) |
810-850 nm (EM5) |
WesternDots 585 |
455-485 nm (EX1) |
568-617 nm (EM2) |
WesternDots 625 |
455-485 nm (EX1) |
568-617 nm (EM2) |
WesternDots 655 |
455-485 nm (EX1) |
675-720 nm (EM3) |
WesternDots 800 |
455-485 nm (EX1) |
800-850 nm (EM5) |
Traditional Dyes and Cyanin |
Excitation channel |
Emission channel |
Fluorescein/FITC |
455-485 nm (EX1) |
508-557 nm (EM1) |
Green Fluorescent Protein (GFP) |
455-485 nm (EX1) |
508-557 nm (EM1) |
Oregon Green |
455-485 nm (EX1) |
508-557 nm (EM1) |
Red Fluorescent Protein (RFP) |
515-545 nm (EX2) |
568-617 nm (EM2) |
Rhodamine 123 |
455-485 nm (EX1) |
515-564 nm (EM1) |
Tetramethyl Rhodamine (TRITC) |
515-545 nm (EX2) |
568-617 nm (EM2) |
Texas Red |
608-632 nm (EX3) |
675-720 nm (EM3) |
Cyanin 2 (Cy2) |
455-485 nm (EX1) |
515-564 nm (EM1) |
Cyanin 3 (Cy3) |
515-545 nm (EX2) |
568-617 nm (EM2) |
Cyanin 5 (Cy5) |
608-632 nm (EX3) |
675-720 nm (EM3) |
Cyanin 5.5 (Cy5.5) |
610-660 nm (EX4) |
710-750 nm (EM4) |
Cyanin 7 (Cy7) |
745-765 nm (EX5) |
800-850 nm (EM5) |
IRDye, StarBright |
Excitation channel |
Emission channel |
IRDye 650 |
608-632 nm (EX3) |
675-720 nm (EM3) |
IRDye 680 |
610-660 nm (EX4) |
710-730 nm (EM4) |
IRDye 700 |
610-660 nm (EX4) |
710-730 nm (EM4) |
IRDye 800 |
745-765 nm (EX5) |
800-850 nm (EM5) |
StarBright Blue 520 |
455-485 nm (EX1) |
508-557 nm (EM1) |
StarBright Blue 700 |
455-485 nm (EX1) |
710-730 nm (EM4) |
Chemiluminescent western blot imaging
Examples of chemiluminescent reagents that can be detected using the iBright FL1500, iBright CL1500, and iBright CL750 Imaging Systems:
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FL1500 |
CL1500 |
CL750 |
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Protein gel imaging
Example protein gel stains that can be detected using the iBright FL1500, iBrightCL1500, and iBright CL750 Imaging Systems:
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FL1500 |
CL1500 |
CL750 |
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Deep purple stains |
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Oriole fluorescent stain |
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Flamingo fluorescent stain |
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Nucleic acid gel imaging
Examples of nucleic acid dyes and stains that can be detected using the iBright FL1500, iBright CL1500, and iBright CL750 Imaging Systems:
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FL1500 |
CL1500 |
CL750 |
Ethidium bromide |
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Hoechst blue |
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Colony counting
Examples of bacterial and mammalian cell colony labeling reagents that can be detected using the iBright FL1500, iBright CL1500, and iBright CL750 Imaging Systems:
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FL1500 |
CL1500 |
CL750 |
GFP (using transillumination) |
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GFP (using epi-illumination) |
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Crystal violet (using transillumination) |
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Crystal violet (using epi-illumination) |
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Blue/white visible (β-galactosidase/X-Gal/IPTG) |
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