Mass Spectrometry Core Essentials | Thermo Fisher Scientific

Proper sample preparation is critical to reproducible and accurate mass spectrometry analysis results. Mass Spectrometry Core Labs strive to offer their users reliable, consistent data. We offer high quality reagents and complete, convenient kits that have been fully tested to enable optimal outcomes, from protein digestion to peptide enrichment, clean-up, and quantitation.

Comprehensive—reagents and kits for every step of the sample preparation workflow, both before and after protein digestion
Versatile—products available in both complete kits and standalone reagents; bulk and custom packaging is also available
Compatible—all kits are designed to be compatible with mass spectrometry analysis, and processed samples have been fully tested and analyzed using Thermo Scientific Mass Spectrometers

Choose the right mass spectrometry sample preparation product

Workflow step	Products
Rapid sample preparation	EasyPep Mini MS Sample Prep Kit	See more products
Abundant protein depletion	High-Select HSA/ Immunoglobulin Depletion Resin (A36368) High-Select Top 14 Abundant Protein Depletion Resin (A36372)	See more products
Protein enrichment	Immunoprecipitation (protein A/G) Immunoprecipitation (streptavidin)	See more products
Protein digestion	Pierce Trypsin Protease, MS Grade (90057) Lys-C Protease, MS Grade (90051) Trypsin/Lys-C Protease Mix, MS-Grade (A41007)	See more products
Peptide enrichment and fractionation	High Select Fe-NTA Phosphopeptide Enrichment Kit (A32992) High Selected TiO₂ Phosphopeptide EnrichmentKit (A32993) Pierce High pH Reversed-Phase Fractionation Kit (84868)	See more products
Peptide desalting	Pierce Peptide Desalting columns (89852) Pierce C18 Tips, 100 µL bed (87784)	See more products
Detergent removal	Pierce Detergent Removal Spin Columns (87777)	See more products
Peptide quantitation	Colorimetric Peptide Quantitation Assay (23275) Fluorometric Peptide Quantitation Assay (23290)	See more products
QC standards	Pierce Peptide Retention Time Calibration Mixture (88321) Pierce BSA Protein Digest Standard (88341) Pierce 6 Protein Digest (88342) Hela Digest Standard (88329) Pierce TMT11plex Yeast Digest Standard (A40939)	See more products
LC-MS solvents	Pierce Trifluoroacetic Acid (TFA) (28904) Piece Formic Acid, LC-MS Grade (28905) Pierce Acetonitrile, LC-MS grade (51101) Pierce Water, LC-MS Grade (51140)	See more products

For protein quantitation applications, learn more about tandem mass tags, SILAC metabolic labeling systems, SureQuant targeted Mass Spec Assays or HeavyPeptide AQUA Custom Synthesis Service.

Featured product data

Rapid sample preparation

Figure 1. The EasyPep Mini MS Sample Prep Kit compared to a traditional protocol and another commercially available kit. The EasyPep Mini MS Sample Prep Kit provides a high peptide yield, and comparable peptide and protein identifications in cell lysates with good reproducibility.

Abundant protein depletion

Figure 2. Abundant protein depletion improves identification of unique proteins. Protein group identification profiles for normal human plasma samples which were (a) not depleted, or depleted using the HSA/Immunoglobulin (b) and Top14 (c) depletion resins, are shown. All samples were reduced/alkylated and digested with trypsin. Samples were analyzed by LC-MS on an Orbitrap Fusion Tribrid mass spectrometer over a 60 minute gradient using 750 ng of sample per injection (performed in triplicate). Raw files were searched against a human protein database using Thermo Scientific Proteome Discoverer 1.4 software. Non-redundant protein group identification numbers are reported for each sample type.

Protein digestion

Figure 3. Comparison of the cleavage selectivity of MS-grade trypsin products. Five-protein mixture samples (10 μg) were digested with Pierce Trypsin Protease or Promega Trypsin Gold for 3 hr and analyzed by LC-MS using a Velos Pro Mass Spectrometer. Data analysis was performed using a Mascot search engine with “no enzyme” digestion settings. Greater than 95% cleavage selectivity for lysine and arginine (K/R) was observed for Pierce Trypsin Protease.

Peptide desalting

Peptide recovery of native and TMT-labeled peptides

Figure 4. Peptide recovery of native peptides at various loads using the Pierce Peptide Desalting Spin Columns. Digested HeLa extracts (5–5,000 μg) were loaded onto Pierce Peptide Desalting Spin Columns and processed according to the protocol. Total recovered desalted peptides were estimated using the Pierce Quantitative Colorimetric Peptide Assay.

Peptide quantitation assay

Figure 5. Sensitivity of the Pierce Quantitative Colorimetric Peptide Assay. (A) Sensitivity of Pierce Quantitative Colorimetric Peptide Assay compared to the Pierce Micro BCA Assay using BSA Digest. (B) Quantitation comparison between Pierce Quantitative Peptide Assays using 12 different cultured mammalian cell lysates.

How to Use video for EasyPep Mini MS Sample Prep Kit

Use this rapid mass spec sample prep kit for protein extraction, digestion and peptide clean-up in 2-4 hours.

Resources

For Research Use Only. Not for use in diagnostic procedures.