PSC Characterization Tools | Thermo Fisher Scientific

Verifying the quality of your pluripotent stem cells (PSCs) is critical to moving your research goals forward. We have a variety of cellular and molecular methods to help you completely and cost-effectively characterize your PSCs. From the PluriTest compatible PrimeView Global Gene Expression Profile Assays, which provides quick verification of pluripotency, to TaqMan hPSC Scorecard Panel, which confirms trilineage differentiation potential, we have the tools you need to characterize with lines with confidence.

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Cellular and molecular PSC characterization methods

	Alkaline Phosphatase Live Stain	Antibody staining	PluriTest-Compatible PrimeView Global Gene Expression Profile Assays	TaqMan hPSC Scorecard Panel	KaryoStat and KaryoStat HD Assays
Product description	Easy identification of pluripotency without compromising cell integrity	Specific and flexible identification of PSCs	Cost-effective global confirmation of pluripotency marker expression	Evaluates pluripotency and confirms trilineage differentiation potential	Alternative to g-banding karyotyping; offers whole-genome coverage for accurate detection of chromosomal abnormalities
How specific are the results?	Low (stains stem and progenitor cells)	Medium (stains human ES and iPS cells)	High (whole-transcriptome gene expression profile)	High (profiles expression of both PSC markers and early germ layer factors)	High
Will the cells remain viable?	Yes	No [1]	No	No	No
How long before I see results?	Stain PSCs typically in 20 minutes or less	Stain PSCs typically in 90–120 minutes	2 days	6–8 hours	3–4 days
Are data analysis tools included?	No	No	Yes, free online PluriTest analysis tool	Yes, free cloud-based software	Yes, free downloadable Chromosome Analysis Suite (ChAS) software
Is a reference standard included?	No	No	Yes [2]	Yes [2]	No
Are EVOS FLoid Cell Imager protocols available?	Yes	Yes	No	N/A	No
Training and expertise required	Minimal	Minimal	Moderate	Moderate	Moderate
Unit size	500 μL vial sufficient for staining twelve 6 cm dishes	100 μg	30 arrays (one sample/array) or 1, 16-sample array plate	1, 384-well plate Kit (4 samples/plate) or 2, 96-well plates (1 sample/plate)	24 arrays (one sample/array)
	Order	Order	Order	Order	Learn more
1. Non-destructive when used with antibodies qualified for live staining.; 2. Reference standard is included in FREE software.

PSC characterization services

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