Artist rendering analytical assay representation

Thermo Fisher Scientific offers a comprehensive CaptureSelect portfolio of biotinylated,HRP and Alexa Fluor conjugated ligands targeting:

  1. Antibodies, fab fragments and related derivatives
  2. Proteins, hormones and biosimilars
  3. Viral vectors
  4. Extracellular vesicles (EVs) like exosomes

The conjugated ligands are available for a variety of analytical assays. The affinity ligand is conjugated to biotin, HRP, or AlexaFluors through a spacer that maintains binding reactivity when immobilized on streptavidin surfaces or used for fluorescent detection.

Contact us  Download application guide


Ligand applications

CaptureSelect conjugated ligands are versatile tools suitable for various applications, including discovery and screening, detection, quantitation, and other product or process analytics. They can be used in:

  • Enzyme-linked immunosorbent assays (ELISA)
  • Bio-Layer Interferometry (BLI) assays
  • Surface Plasmon Resonance (SPR) assays
  • Immunoprecipitation
  • Western blot
  • Protein capture using magnetic beads


For examples, protocols, and publications highlighting the conjugated ligands, download the application guide or watch the webinar on the versatility of single domain antibody technology.

Rendering of CaptureSelect ligand conjugate bonded to target protein

Figure 1. Representation of a CaptureSelect biotinylated ligand bound to its target protein. Biotin molecules are chemically linked to the affinity ligand with a spacer, enabling immobilization on streptavidin surfaces.


CaptureSelect products

You can find more detailed information on the selectivity and use of these products in the application guide or by viewing the specific product pages linked below.

CaptureSelect conjugated ligands for antibodies and antibody fragments

CaptureSelect ligandBiotinBiotin
100 µg500 µg
Anti-IgG-Fc (human)71032621007103262500
Anti-IgG-CH171032021007103202500
Anti-LC-Kappa(human)71032721007103272500
Anti-LC-Lambda(human)71030821007103082500
Anti-Free LC-Kappa(human)71032921007103292500
Anti-IgG371030421007103042500
Anti-IgG471029021007102902500
Anti-IgA71028821007102882500
Anti-IgM71028921007102892500
Anti-IgE71035421007103542500
Anti-IgG-FC(multi-species)71028521007102852500
Anti-LC-Kappa(murine)71031521007103152500
Anti-LC-Lambda(mouse)71032321007103232500
Anti-IgG-FC (Rabbit)71036421007103642500

CaptureSelect conjugated ligands for proteins and hormones

CaptureSelect ligandBiotinBiotin
100 µg500 µg
Anti-Factor VII7102992100 7102992500
Anti-Factor VIII7102862100 7102862500
Anti-Factor IX7103002100 7103002500
Anti-Factor X7103702100 7103702500
Anti-AAT7102872100 7102872500
Anti-Fibrinogen7102912100 7102912500
Anti-HSA7102972100 7102972100
Anti-Transferrin7103062100 7103062500
Anti-HGH7103160100 7103160500
Anti-FSH7103180100 7103180500
Anti-Prothrombin7103452100 7103452500
Anti-EPO7103372100 7103372500
Anti-C1-Inhibitor7103402100 7103402500
Anti-Gonadotropin7103412100 7103412500

Recombinant protein detection and quantitation using C-tag and CaptureSelect conjugated ligands for viral vectors

CaptureSelect LigandAnti-C-tagAnti-AAVXAnti-AAV8Anti-AAV9Anti-AdV5
Biotin100 µg71032521007103522100710338210071033321007103532100
Biotin500 µg71032525007103522500710338250071033325007103532500
HRP100 µg7303072100----
HRP100 µL-7303522100-7303332100-
HRP500 µL-7303522500-7303332500-
Alexa Fluor 488-7213252100----
Alexa Fluor 647100 µg-7233522100-7233322100-

CaptureSelect conjugated ligands for kinetic experiments

CaptureSelect ligandBiotinBiotin
100 µg500 µg
Anti-IgG-FC PK71033221007103322500
Anti-Human Fab-Kappa Kinetics71033021007103302500
Anti-Human Fab-Lambda Kinetics71033121007103312500

CaptureSelect conjugated ligands for exosomes

CaptureSelect ligandBiotinBiotinHRPHRP
100 µg500 µg100 µL500 µL
Anti-CD817103982100710398250073039821007303982500
Anti-CD637103992100710399250073039921007303992500
Anti-CD97104002100710400250073040021007304002500

CaptureSelect conjugated ligands for antibodies and antibody fragments

CaptureSelect ligandBiotinBiotin
100 µg500 µg
Anti-IgG-Fc (human)71032621007103262500
Anti-IgG-CH171032021007103202500
Anti-LC-Kappa(human)71032721007103272500
Anti-LC-Lambda(human)71030821007103082500
Anti-Free LC-Kappa(human)71032921007103292500
Anti-IgG371030421007103042500
Anti-IgG471029021007102902500
Anti-IgA71028821007102882500
Anti-IgM71028921007102892500
Anti-IgE71035421007103542500
Anti-IgG-FC(multi-species)71028521007102852500
Anti-LC-Kappa(murine)71031521007103152500
Anti-LC-Lambda(mouse)71032321007103232500
Anti-IgG-FC (Rabbit)71036421007103642500

CaptureSelect conjugated ligands for proteins and hormones

CaptureSelect ligandBiotinBiotin
100 µg500 µg
Anti-Factor VII7102992100 7102992500
Anti-Factor VIII7102862100 7102862500
Anti-Factor IX7103002100 7103002500
Anti-Factor X7103702100 7103702500
Anti-AAT7102872100 7102872500
Anti-Fibrinogen7102912100 7102912500
Anti-HSA7102972100 7102972100
Anti-Transferrin7103062100 7103062500
Anti-HGH7103160100 7103160500
Anti-FSH7103180100 7103180500
Anti-Prothrombin7103452100 7103452500
Anti-EPO7103372100 7103372500
Anti-C1-Inhibitor7103402100 7103402500
Anti-Gonadotropin7103412100 7103412500

Recombinant protein detection and quantitation using C-tag and CaptureSelect conjugated ligands for viral vectors

CaptureSelect LigandAnti-C-tagAnti-AAVXAnti-AAV8Anti-AAV9Anti-AdV5
Biotin100 µg71032521007103522100710338210071033321007103532100
Biotin500 µg71032525007103522500710338250071033325007103532500
HRP100 µg7303072100----
HRP100 µL-7303522100-7303332100-
HRP500 µL-7303522500-7303332500-
Alexa Fluor 488-7213252100----
Alexa Fluor 647100 µg-7233522100-7233322100-

CaptureSelect conjugated ligands for kinetic experiments

CaptureSelect ligandBiotinBiotin
100 µg500 µg
Anti-IgG-FC PK71033221007103322500
Anti-Human Fab-Kappa Kinetics71033021007103302500
Anti-Human Fab-Lambda Kinetics71033121007103312500

CaptureSelect conjugated ligands for exosomes

CaptureSelect ligandBiotinBiotinHRPHRP
100 µg500 µg100 µL500 µL
Anti-CD817103982100710398250073039821007303982500
Anti-CD637103992100710399250073039921007303992500
Anti-CD97104002100710400250073040021007304002500

CaptureSelect biotinylated ligands:

Review our list of biotinylated ligands directed against antibodies and related derivatives. Find more detailed information on selectivity and use of these products in the product information sheets available for download on the specific product pages:

Biotin Anti-IgG-CH1

Binds human IgG and all Fab fragments thereof, through recognition of the CH1 domain on all four human IgG subclasses.

Biotin Anti-IgG-Fc (Human)

Binds human IgG, through recognition of the Fc region (CH3 domain) on all four human IgG subclasses.

Biotin Anti-IgG-Fc (Multi-species)

Binds IgG through recognition of the Fc region on IgG of multiple species. View product sheet for species coverage.
Biotin Anti-IgG-Fc (Rabbit)Binds to rabbit IgG (immunoglobulin G) from recombinant and plasma sources. The ligand shows no cross-binding to bovine, horse, sheep, goat, mouse, rat, and human IgG. Moderate cross-binding was observed towards swine IgG.

Biotin Anti-IgA

Binds human IgA, IgA-dimers and secretory IgA (sIgA), through recognition of the Fc region on both human IgA subclasses.

Biotin Anti-IgM

Binds human, mouse and rat IgM.
Biotin Anti-IgEbinds to human IgE (immunoglobulin E) from recombinant and plasma sources, through recognition of the Fc region (CH4 domain)

Biotin Anti-LC-lambda (Human)

Binds all human lambda light chains (LC), through recognition of the constant domain (CL-lambda) on any human antibody, Fab fragment or free light chain.

Biotin Anti-LC-kappa (Human)

Binds all human kappa light chains (LC), through recognition of the constant domain (CL-kappa) on any human antibody, Fab fragment or free light chain.   

Biotin Anti-LC-kappa (Murine)

Binds all mouse - and rat kappa light chains (LC), through recognition of the constant domain (CL-kappa) on any mouse and rat antibody, Fab fragment or free light chain.
Biotin Anti-LC-lambda (Mouse) Binds all mouse lambda light chains (LC), through recognition of the constant domain (CL-lambda) on any mouse antibody, Fab fragment or free light chain
Biotin Anti-LC-lambda (Rat) Binds all rat lambda light chains (LC), through recognition of the constant domain (CL-lambda) on any rat antibody, Fab fragment or free light chain

Biotin Anti-IgG3 (Human)

Binds human IgG3 without any cross-binding to other human IgG subclasses or antibody isotypes.

Biotin Anti-IgG4 (Human)

Binds human IgG4 without any cross-binding to other human IgG subclasses or antibody isotypes.

Biotin Human Fab-kappa Kinetics

High affinity capture of human IgG Fab fragments that possess a kappa light chain. Extreme low off-rates are achieved through avidity by targeting the CL-kappa domain of the Fab (light chain) and its IgG CH1 domain (heavy chain) simultaneously, thereby enabling Fab screening on target binding kinetics.

Biotin Human Fab-lambda Kinetics

High affinity capture of human IgG Fab fragments that possess a lambda light chain. Extreme low off-rates are achieved through avidity by targeting the CL-lambda domain of the Fab (light chain) and its IgG CH1 domain (heavy chain) simultaneously, thereby enabling Fab screening on target binding kinetics.

Biotin Anti-Free LC-kappa (Human)

Binds highly selective to free human kappa light chains and dimers thereof, through recognition of an epitope on the human CL-kappa domain that is masked in intact antibodies and Fab fragments, thereby providing a unique tool for specific detection and quantitation of free kappa light chains in (crude) antibody preps.

Biotin Human IgG-Fc PK

Binds highly selective to human IgG, through recognition of the Fc region on all four human IgG subclasses without cross-binding to mouse, rat, rhesus and cynomolgus monkey IgG, thereby making it extremely suitable for setting up pharmacokinetics (PK) assays.

Review our list of biotinylated ligands directed against different proteins and virus particles. Find more detailed information on selectivity and use of these products in the product information sheets available for download on the specific product pages:

Biotin Anti-C-tag

Binds the short C-tag sequence E-P-E-A (glutamic acid - proline - glutamic acid – alanine) when this affinity tag is fused at the C-terminus of a target protein.

Alexa Fluor 488 Anti-C-tag

Binds the short C-tag sequence E-P-E-A (glutamic acid - proline - glutamic acid – alanine) when this affinity tag is fused at the C-terminus of a target protein.
Biotin Anti-FXBinds to human Factor X and its activated form Factor Xa from plasma and recombinant sources

Biotin Anti-FIX

Binds recombinant as well as plasma derived human Factor IX (FIX)

Biotin Anti-FVIII

Binds recombinant (full length and B-domain deleted) as well as plasma derived human Factor VIII (FVIII), through recognition of the FVIII light chain.
Biotin anti-FVIIBinds recombinant as well as plasma derived human Factor VII (FVII)
Biotin Anti-Gonadotropin Binds to the alpha chain of recombinant as well as natural sourced hCG (human chorionic gonadotropin), LH (luteinizing hormone), TSH (Thyroid-stimulating hormone) and FSH (follicle-stimulating hormone)
Biotin Anti-Human Albumin Binds recombinant as well as natural sourced human serum albumin (HSA) including HSA-fusion proteins
Biotin Anti-AAT Binds recombinant as well as natural sourced human α1-Antitrypsin (AAT)
Biotin Anti-C1-Inhibitor Binds recombinant as well as natural sourced human C1-Inhibitor (C1-inh, C1 esterase inhibitor)
Biotin Anti-FibrinogenBinds recombinant as well as natural sourced human Fibrinogen (coagulation factor I, FI)
Biotin Anti-Transferrin Binds recombinant as well as natural sourced human Transferrin
Biotin Anti-α1-Acid Glycoprotein Binds recombinant as well as natural sourced human α1-Acid Glycoprotein (AGP) or Orosomucoid (ORM)
Biotin Anti-Haptoglobin Binds recombinant as well as natural sourced human Haptoglobin (Hp)
Biotin Anti-C3 Binds recombinant as well as natural sourced human Complement Component 3 (C3)
Biotin Anti-vWF Binds recombinant as well as natural sourced human von Willebrand Factor (vWF)
Biotin Anti-ApoA1 Binds recombinant as well as natural sourced human Apolipoprotein A1 (ApoA1)
Biotin Anti-α2-Macroglobulin Binds recombinant as well as natural sourced human α2-Macroglobulin (A2M)
Biotin Anti-Antithrombin III Binds recombinant as well as natural sourced human Antithrombin III (ATIII)
Biotin Anti-Prothrombin Binds recombinant as well as natural sourced human Prothrombin (coagulation factor II, FII)

Biotin Anti-hGH

Binds recombinant as well as natural sourced human Growth Hormone (hGH).

Biotin Anti-GCSF

Binds recombinant as well as natural sourced human Granulocyte-Colony Stimulating Factor (G-CSF)

Biotin Anti-FSH

Binds recombinant as well as natural sourced human Follicle Stimulating Hormone (FSH), through recognition of an epitope specific for intact FSH only.
Biotin Anti-Insulin
Binds recombinant and natural-sourced human insulin and shows similar binding reactivity towards porcine- and bovine-sourced insulin. It also binds to human proinsulin, the precursor of insulin that still contains the C-peptide.
Biotin Anti-EPOBinds recombinant and natural-sourced human Erythropoietin (EPO).
Biotin anti-AAV8Binds with high affinity to Adeno-associated virus particles of serotype 8 (AAV8). Note that it may cross-bind towards other AAV serotypes.
Biotin anti-AAV9Binds with high affinity and selectivity to Adeno-associated virus particles of serotype 9 (AAV9)
Biotin anti-AAVXBinds with high affinity and selectivity to adeno-associated viral (AAV) particles of a wide variety of serotypes.
Biotin anti-Adv5Binds with high affinity and selectivity to recombinant hexon capsomeres of Adenovirus serotype 5 (AdV5) particles as well as free hexon proteins thereof

Review our list of biotinylated ligands directed against several tetraspanin family member exosome surface molecules. Find more detailed information on selectivity and use of these products in the product information sheets available for download on the specific product pages:

Biotin Anti-CD81

Binds the tetraspanin molecule CD81 (Cluster of Differentiation 81; TAPA1 or TSPAN28) expressed on extracellular vesicles (EVs) like exosomes.

Biotin Anti-CD63

Binds the tetraspanin molecule CD63 (Cluster of Differentiation 63; LAMP3 or LIMP1) expressed on extracellular vesicles (EVs) like exosomes.

Biotin Anti-CD9

Binds the tetraspanin molecule CD9 (Cluster of Differentiation 9; MIC3, MRP1 or TSPAN29) expressed on extracellular vesicles (EVs)  like exosomes.

Review our list of biotinylated ligands directed against antibodies and related derivatives. Find more detailed information on selectivity and use of these products in the product information sheets available for download on the specific product pages:

Biotin Anti-IgG-CH1

Binds human IgG and all Fab fragments thereof, through recognition of the CH1 domain on all four human IgG subclasses.

Biotin Anti-IgG-Fc (Human)

Binds human IgG, through recognition of the Fc region (CH3 domain) on all four human IgG subclasses.

Biotin Anti-IgG-Fc (Multi-species)

Binds IgG through recognition of the Fc region on IgG of multiple species. View product sheet for species coverage.
Biotin Anti-IgG-Fc (Rabbit)Binds to rabbit IgG (immunoglobulin G) from recombinant and plasma sources. The ligand shows no cross-binding to bovine, horse, sheep, goat, mouse, rat, and human IgG. Moderate cross-binding was observed towards swine IgG.

Biotin Anti-IgA

Binds human IgA, IgA-dimers and secretory IgA (sIgA), through recognition of the Fc region on both human IgA subclasses.

Biotin Anti-IgM

Binds human, mouse and rat IgM.
Biotin Anti-IgEbinds to human IgE (immunoglobulin E) from recombinant and plasma sources, through recognition of the Fc region (CH4 domain)

Biotin Anti-LC-lambda (Human)

Binds all human lambda light chains (LC), through recognition of the constant domain (CL-lambda) on any human antibody, Fab fragment or free light chain.

Biotin Anti-LC-kappa (Human)

Binds all human kappa light chains (LC), through recognition of the constant domain (CL-kappa) on any human antibody, Fab fragment or free light chain.   

Biotin Anti-LC-kappa (Murine)

Binds all mouse - and rat kappa light chains (LC), through recognition of the constant domain (CL-kappa) on any mouse and rat antibody, Fab fragment or free light chain.
Biotin Anti-LC-lambda (Mouse) Binds all mouse lambda light chains (LC), through recognition of the constant domain (CL-lambda) on any mouse antibody, Fab fragment or free light chain
Biotin Anti-LC-lambda (Rat) Binds all rat lambda light chains (LC), through recognition of the constant domain (CL-lambda) on any rat antibody, Fab fragment or free light chain

Biotin Anti-IgG3 (Human)

Binds human IgG3 without any cross-binding to other human IgG subclasses or antibody isotypes.

Biotin Anti-IgG4 (Human)

Binds human IgG4 without any cross-binding to other human IgG subclasses or antibody isotypes.

Biotin Human Fab-kappa Kinetics

High affinity capture of human IgG Fab fragments that possess a kappa light chain. Extreme low off-rates are achieved through avidity by targeting the CL-kappa domain of the Fab (light chain) and its IgG CH1 domain (heavy chain) simultaneously, thereby enabling Fab screening on target binding kinetics.

Biotin Human Fab-lambda Kinetics

High affinity capture of human IgG Fab fragments that possess a lambda light chain. Extreme low off-rates are achieved through avidity by targeting the CL-lambda domain of the Fab (light chain) and its IgG CH1 domain (heavy chain) simultaneously, thereby enabling Fab screening on target binding kinetics.

Biotin Anti-Free LC-kappa (Human)

Binds highly selective to free human kappa light chains and dimers thereof, through recognition of an epitope on the human CL-kappa domain that is masked in intact antibodies and Fab fragments, thereby providing a unique tool for specific detection and quantitation of free kappa light chains in (crude) antibody preps.

Biotin Human IgG-Fc PK

Binds highly selective to human IgG, through recognition of the Fc region on all four human IgG subclasses without cross-binding to mouse, rat, rhesus and cynomolgus monkey IgG, thereby making it extremely suitable for setting up pharmacokinetics (PK) assays.

Review our list of biotinylated ligands directed against different proteins and virus particles. Find more detailed information on selectivity and use of these products in the product information sheets available for download on the specific product pages:

Biotin Anti-C-tag

Binds the short C-tag sequence E-P-E-A (glutamic acid - proline - glutamic acid – alanine) when this affinity tag is fused at the C-terminus of a target protein.

Alexa Fluor 488 Anti-C-tag

Binds the short C-tag sequence E-P-E-A (glutamic acid - proline - glutamic acid – alanine) when this affinity tag is fused at the C-terminus of a target protein.
Biotin Anti-FXBinds to human Factor X and its activated form Factor Xa from plasma and recombinant sources

Biotin Anti-FIX

Binds recombinant as well as plasma derived human Factor IX (FIX)

Biotin Anti-FVIII

Binds recombinant (full length and B-domain deleted) as well as plasma derived human Factor VIII (FVIII), through recognition of the FVIII light chain.
Biotin anti-FVIIBinds recombinant as well as plasma derived human Factor VII (FVII)
Biotin Anti-Gonadotropin Binds to the alpha chain of recombinant as well as natural sourced hCG (human chorionic gonadotropin), LH (luteinizing hormone), TSH (Thyroid-stimulating hormone) and FSH (follicle-stimulating hormone)
Biotin Anti-Human Albumin Binds recombinant as well as natural sourced human serum albumin (HSA) including HSA-fusion proteins
Biotin Anti-AAT Binds recombinant as well as natural sourced human α1-Antitrypsin (AAT)
Biotin Anti-C1-Inhibitor Binds recombinant as well as natural sourced human C1-Inhibitor (C1-inh, C1 esterase inhibitor)
Biotin Anti-FibrinogenBinds recombinant as well as natural sourced human Fibrinogen (coagulation factor I, FI)
Biotin Anti-Transferrin Binds recombinant as well as natural sourced human Transferrin
Biotin Anti-α1-Acid Glycoprotein Binds recombinant as well as natural sourced human α1-Acid Glycoprotein (AGP) or Orosomucoid (ORM)
Biotin Anti-Haptoglobin Binds recombinant as well as natural sourced human Haptoglobin (Hp)
Biotin Anti-C3 Binds recombinant as well as natural sourced human Complement Component 3 (C3)
Biotin Anti-vWF Binds recombinant as well as natural sourced human von Willebrand Factor (vWF)
Biotin Anti-ApoA1 Binds recombinant as well as natural sourced human Apolipoprotein A1 (ApoA1)
Biotin Anti-α2-Macroglobulin Binds recombinant as well as natural sourced human α2-Macroglobulin (A2M)
Biotin Anti-Antithrombin III Binds recombinant as well as natural sourced human Antithrombin III (ATIII)
Biotin Anti-Prothrombin Binds recombinant as well as natural sourced human Prothrombin (coagulation factor II, FII)

Biotin Anti-hGH

Binds recombinant as well as natural sourced human Growth Hormone (hGH).

Biotin Anti-GCSF

Binds recombinant as well as natural sourced human Granulocyte-Colony Stimulating Factor (G-CSF)

Biotin Anti-FSH

Binds recombinant as well as natural sourced human Follicle Stimulating Hormone (FSH), through recognition of an epitope specific for intact FSH only.
Biotin Anti-Insulin
Binds recombinant and natural-sourced human insulin and shows similar binding reactivity towards porcine- and bovine-sourced insulin. It also binds to human proinsulin, the precursor of insulin that still contains the C-peptide.
Biotin Anti-EPOBinds recombinant and natural-sourced human Erythropoietin (EPO).
Biotin anti-AAV8Binds with high affinity to Adeno-associated virus particles of serotype 8 (AAV8). Note that it may cross-bind towards other AAV serotypes.
Biotin anti-AAV9Binds with high affinity and selectivity to Adeno-associated virus particles of serotype 9 (AAV9)
Biotin anti-AAVXBinds with high affinity and selectivity to adeno-associated viral (AAV) particles of a wide variety of serotypes.
Biotin anti-Adv5Binds with high affinity and selectivity to recombinant hexon capsomeres of Adenovirus serotype 5 (AdV5) particles as well as free hexon proteins thereof

Review our list of biotinylated ligands directed against several tetraspanin family member exosome surface molecules. Find more detailed information on selectivity and use of these products in the product information sheets available for download on the specific product pages:

Biotin Anti-CD81

Binds the tetraspanin molecule CD81 (Cluster of Differentiation 81; TAPA1 or TSPAN28) expressed on extracellular vesicles (EVs) like exosomes.

Biotin Anti-CD63

Binds the tetraspanin molecule CD63 (Cluster of Differentiation 63; LAMP3 or LIMP1) expressed on extracellular vesicles (EVs) like exosomes.

Biotin Anti-CD9

Binds the tetraspanin molecule CD9 (Cluster of Differentiation 9; MIC3, MRP1 or TSPAN29) expressed on extracellular vesicles (EVs)  like exosomes.

Bioprocessing resources

Access a targeted collection of scientific application notes, case studies, posters, white papers and more for bioprocessing:

For Research Use or further manufacturing, not for diagnostic use or direct administration in humans or animals.