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| Highest purity for molecular assays | Pure flow compatible cells | Untouched—most physiological relevant cells | |
|---|---|---|---|
| Dynabeads CD19 | Dynabeads CD19 + DETACHaBEAD CD19 (sold separately) | Dynabeads Untouched Human B Cells | |
| Isolation method | Depletion or positive isolation | Positive isolation | Negative isolation |
| Starting samples | Any sample (whole blood, MNC, bone marrow) | Any sample (whole blood, MNC, bone marrow) | MNC* |
| # MNC processed (mL whole blood processed) | 1–2 x 109 MNC 200 mL whole blood | 1–2 x 109 MNC 80 mL whole blood | 1 x 109 cells |
| Purity | Highest 98-100% | Highest 98-100% | High 90-95% |
| Yield | Highest 95-100% | Standard 50-70% | Standard 50-70% |
| Viability | >95% | >95% | >95% |
| Isolation time | ~30 min | ~90 min | ~50 min |
| Isolation temp. | Cold | Release at room temp. | Cold |
| Applications | Depletion of unwanted B cells or positive isolation for molecular applications, e.g., isolation of proteins & nucleic acids | Cellular assays (cell culture, cell activation/proliferation, cytokine studies) Translational research Molecular isolation of proteins & nucleic acids | Cellular applications for physiological studies (functional studies, flow analysis, study of resting/activated cells) |
| Bead-free target cells | No | Yes (and antibody-free) | Yes |
| Compatible with flow cytometry/sorting | No | Yes | Yes |
| Shelf life from production date | 3 years | 3 years | 2 years |
| Required materials | Magnets | Magnets | Magnets |
| Order now | Order now | Order now |
* MNC: Mononuclear cells
Dynabeads CD19 Pan B are super-paramagnetic beads covalently coupled with anti-human CD19 antibody to enable isolation or depletion of human CD19+ B cells from whole blood, buffy coat, bone marrow, mononuclear cells (MNC), and tissue digests.
Isolate B cells with Dynabeads CD19 and release them with DETACHaBEAD CD19 for pure, viable, phenotypically unaltered and unactivated B cells. Prepare for downstream applications including sorting, culturing studies, and flow cytometry.
Dynabeads Untouched Human B Cells Kits are capable of extracting untouched B cells from PBMC through a method of negative isolation. The kit depletes T cells, dendritic cells, NK cells, monocytes, granulocytes, erythrocytes, and platelets. The negatively isolated human B cells are left in the sample and have not been in contact with the Dynabeads. The bead- and antibody-free B cells are left and in perfect shape for any functional assay and downstream application.
THIS IS FOR HUMAN RESEARCH—NOT FOR MOUSE