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ProtoArray services and its associated products have been discontinued, information here is for reference.
Reliable predictors of disease—autoantibody markers provide a disease-specific fingerprint for cancers as well as autoimmune and other diseases.
Available—autoantibodies are highly enriched in easily accessible biological fluids such as serum, plasma, tears, and saliva.
Stable and easy to detect—their long half-lives and sensitive detection strategy provide a simple, robust solution for rapidly detecting autoantibodies.
Over 9,000 full-length human proteins are displayed on the ProtoArray® Human Protein Microarray v5.0. Proteins are expressed in insect cells as N-terminal GST fusion proteins using a baculovirus expression system. All proteins are purified under non-denaturing conditions and printed at 4°C to preserve native protein structure.
Review a list of all human proteins on the ProtoArray version 5.0 microarray
ProtoArray® Human Protein Microarray v5.0 utilizes a simple, rapid protocol for the sensitive (LOD < 1 ng/μl) detection of autoantibodies against many autoantigens. In a single day, you can profile up to a hundred patient samples against thousands of unique human antigens.
Review the ProtoArray profiling protocol
ProtoArray® Human Protein Microarrays v5.0 provides absolute identification of a patient's autoantibodies. The identity of every antigen printed on the array is known and addressable. The array includes specific controls (e.g. human IgG, anti-human IgG, human IgA) to monitor assay quality and normalize data, leading to accurate autoantibody detection.
ProtoArray® Prospector, a freeware tool, can be used for the analysis of data from immune response biomarker profiling assays. ProtoArray® Prospector:
Download the ProtoArray® Prospector v5.2 software
Invitrogen's ProtoArray® services team of highly trained scientists are experts in experimental design and statistical analysis. They can perform the experiments for you and prepare an extensive report.
Learn more about Invitrogen's ProtoArray® Services
For your convenience, we have compiled references that describe applications of this powerful technology.
See ProtoArray® technology Literature Citations
Contact our technical support specialists at (800) 955-6288 (US only) or e-mail at techsupport@invitrogen.com.
For Research Use Only. Not for use in diagnostic procedures.