Overview

There are three models in the iBright Imaging Systems family: The iBright CL750 Imaging System, the iBright CL1500 Imaging System, and the iBright FL1500 Imaging System. The iBright CL750 Imaging System offers the core essential western blot and gel imaging functions and makes the transition from the darkroom and film easy. The iBright CL1500 Imaging System expands application support and has many of the high-performance specifications of our premier iBright FL1500 model. The iBright FL1500 Imaging System features maximum application support, including fluorescence western blot imaging with up to four fluorescence channels at a time.

Specifications

 

 

iBright CL750 Imaging System

 

iBright CL1500 Imaging System

 

iBright FL1500 Imaging System
 

Core essential western blot and gel imaging-transition from darkroom and film-based detection with ease

Expanded application support with many of the same high-performance specifications as the iBright FL1500 imager

Maximum application support including up to 4-channel multiplex western blot imaging
Main supported imaging applications
Colorimetric stained protein gels

Fluorescent stained protein gels

Fluorescent stained nucleic acid gels

Colorimetric stained membranes

Limited*

Chemiluminescent western blots

Colorimetric western blots

 

Fluorescent western blots

 

 

Qualitative visible imaging applications**
Translucent objects (e.g., colony plates)

Opaque objects (e.g., 2D strips, TLC plates, leaf sections)

 

GFP expression in multiwell plates (e.g., 6-well)  

 

Camera
Detector Cooled 16-bit CCD, 65,535 shades of gray
Resolution 9.1 megapixels
Lens Fixed, 25 mm, f/0.95
Field of view 22.5 x 18.0 cm (W x D) (image up to 4 mini blots or gels)
Binning modes 1x1, 2x2, 4x4 (high-resolution/sensitivity settings)

1x1, 2x2, 3x3, 4x4, 5x5, 6x6, 8x8 (maximum flexibility for adjusting resolution and sensitivity based on needs)
Zoom 1–2x, digital (digital zoom reduces the effective resolution of the zoomed image) 1–8x (1–2x mechanical + 1–4x digital) (Mechanical zoom maximizes sensitivity by moving the camera closer to the sample stage, reducing focal length)
System interface
Touchscreen 12.1 inch capacitive LCD display, 1,024 x 768 pixels
Storage and connectivity
USB 2 USB (2.0)
Network Ethernet port, Connect (cloud-based) connectivity, optional Wi-Fi (adapter sold separately)
Image file format G2i (proprietary), TIFF, JPG, PNG
Hard drive 64 GB SSD 256 GB SSD
System Hardware
Sample drawer and stage Manually operated drawer with fixed stage

Automatic open/close drawer with automatic rotating stage (up to 10°)
Filter set 2 filters (0 excitation, 2 emission)

4 filters (2 excitation, 2 emission)

12 filter (6 excitation, 6 emission)
Illumination source
  • Green LED (470–550 nm) transilluminator
  • Green LED (470–550 nm) transilluminator
  • Epi white LED
  • Green LED (470–550 nm) transilluminator 
  • Epi white LED
  • Epi near-IR LED
System software
Automated features
  • Automatic zoom
  • Automatic focus
  • Automatic exposure (Smart Exposure)
  • Automatic on-board image analysis
Stand-alone analysis applications

iBright Analysis Software: desktop version (macOS™ or Windows™ operating systems) and Connect (cloud-based) version

iBright Analysis Software—Secure: (macOS™ or Windows™ operating systems) for 21 CFR part 11 compliance support
Shipping, warranty, and upgrades
Dimensions

(L x W x H): 68 x 38 x 60 cm
Weight

Approximately 47 kg (105 lb)

Approximately 50 kg (110 lbs)
Warranty 1 year from date of purchase

2 years from date of purchase
Upgradability No
 Yes, to FL1500 imager

No

* Membranes must be imaged when wet, using Thermo Scientific Pierce Reversible Protein Stain Kit for Nitrocellulose Membranes or Thermo Scientific Pierce Reversible Protein Stain Kit for PVDF Membranes.


** Applications provide a qualitative visualization of the object or confirmation of the presence of signal. Not recommended for quantitation.

 

*** Smart Range HDR available in chemiluminescent blot mode only.