Primer Designer Tool for PCR & Sanger Sequencing

Streamline your workflow with our primer design tool

From design to synthesis, quality primers are vital to successful results. Use our online Applied Biosystems™ Primer Designer™ Tool to search for the right PCR/Sanger sequencing primer pair from a database of ~650,000 predesigned primer pairs for resequencing the human exome and human mitochondrial genome. Choose from different amplicon lengths to accommodate various research applications and biological sample types.

• Our primers are free of known SNPs and primer-dimers, highly target-specific and used under universal PCR conditions 
• Full primer coverage for Ion Torrent™ AmpliSeq™ Exome Panel and Ion Torrent™ AmpliSeq™ Cancer Hotspot Panel v.2 Sanger confirmation workflow
• Flexible primer configuration to meet your research needs: primers can be ordered unmodified, M13-tailed, HPLC-purified or desalted
• All the primers have been checked by mass spectrometry and have passed stringent bioinformatics metrics; lab bench validation tests have shown >95% success rate

How to Use the Primer Designer™ Tool

No time for primer design?

From design to synthesis, quality primers are vital to successful results. Search our tool, to find and order pre-designed primers for PCR and Sanger sequencing. Free of SNPs and primer-dimers, highly target-specific, and used under universal PCR conditions - these primers can be ordered unmodified, M13-tailed, HPLC-purified, or desalted. All are checked by mass spectometry. Have confidence knowing primer pairs are selected to tile across exons for optimal exon coverage with a minimal number of primer pairs.

Save time with the Primer Designer™ Tool by selecting from >300,000 primer pairs targeting the human exome.

(File must be less than 500 kb in size and contain less than 1000 unique line-items.)

For Research Use Only. Not for human or animal therapeutic or diagnostic use.