Fluorescence measurement

Fluorescence measurement for DNA quantification offers higher sensitivity and specificity compared to UV absorbance. We offer two kinds of fluorescence-based assays for DNA quantification: Qubit DNA Assays Kits and Quant-iT DNA Assays Kits. Find the optimal kit based on your throughput needs and instrument availability.

 

Qubit DNA Assay Kits

 

Qubit DNA assay kits are broadly categorized as double-stranded DNA (dsDNA) assays or single-stranded DNA (ssDNA) assays. These assays are designed to work with Qubit fluorometers.

Select your sample concentration  

Qubit 1X dsDNA HS Assay Kit

 

  • Highly selective for double-stranded DNA (dsDNA) over RNA
  • Designed to be accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL
  • Provides reagent and buffer in a ready-to-use formulation
Qubit dsDNA HS Assay Kit
Q33130
  • Highly selective for double-stranded DNA over RNA
  • Designed to be accurate for initial sample concentrations from 100 pg/µL to 1,000 ng/µL
  • Provides concentrated assay reagent dilution buffer, and pre-diluted DNA standards
Qubit ssDNA Assay Kit
  • Ideal for quantifying single-stranded DNA or oligonucleotides
  • Provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards
Qubit dsDNA BR Assay Kit

 

  • Highly selective for double-stranded DNA (dsDNA) over RNA
  • Designed to be accurate for initial sample concentrations from 100 pg/µL to 1,000 ng/µL
  • Provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards
Qubit 1X dsDNA BR Assay Kit

 

  • Highly selective for dsDNA over ssDNA, RNA, and protein
  • Designed to be accurate for initial DNA sample concentrations of 200 pg/μL to 4,000 ng/μL
  • Provides a ready-to-use working solution

Need a Qubit fluorometer?

Choose the Qubit fluorometer that's right for you. Learn more.

Quant-iT DNA Assays Kits

Quant-iT DNA Assay Kits are designed to use with microplate readers for DNA quantification. Each kit offers sufficient reagents to perform 1,000 assays, based on a 200 µL assay volume in a 96-well microplate format.

Select your sample concentration  

Quant-iT dsDNA HS Assay
  • Highly selective for double-stranded DNA over RNA
  • Designed to be accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL
  • Provides concentrated assay reagent dilution buffer, and pre-diluted DNA standards
Quant-iT Broad-Range dsDNA HS Assay
  • Highly selective for double-stranded DNA over RNA
  • Designed to be accurate for initial sample concentrations from 100 pg/µL to 1,000 ng/µL
  • Provides concentrated assay reagent dilution buffer, and pre-diluted DNA standards
Quant-iT 1X dsDNA BR Assay
Q33267-650x600.jpg-650
  • Highly selective for dsDNA, RNA, and protein
  • Designed to be accurate for initial sample concentrations from 200 pg/µL to 4,000 ng/µL
  • Provides reagent and buffer in a ready-to-use formulation

UV-Vis measurement

UV-Vis absorbance is a commonly used method for routine DNA quantification. It is simple and convenient to use as no sample preparation, dyes, or standards are required.

 

NanoDrop Spectrophotometers use UV absorbance measurement and allow you to quantify and quality DNA, RNA, and protein samples in seconds with 1–2 µL of sample volume.

 

The NanoDrop instruments require no assay kits, but you may be able to increase your productivity using a kit.

Productivity Kit for NanoDrop One/OneC
  • NanoDrop-branded Finnpipette F1 Adjustable Volume Pipette , 0.2–2µL
  • Finntip Pipette Tips, 10 µL, sterile, pack of 96
  • Microfiber screen wipe for NanoDrop OneC Spectrophotometer
  • NanoDrop PR-1 Reconditioning Compound Kit
  • NanoDrop PV-1 Performance Verification Solution
  • 10 mm path quartz cuvette
  • Cuvette stir bars, 5 x 2mm, pack of 10

Supply your experiment with other trusted Everyday Essentials

 

Qubit dsDNA HS Assay Kit

  • Highly selective for double-stranded DNA (dsDNA) over RNA
  • Designed to be accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL
  • Provides concentrated assay reagent, dilution buffer, and pre-diluted DNA standards

Need a NanoDrop instrument?

Choose the NanoDrop instrument that's right for you. Learn more.

Need more information?

Quantification of the DNA in your sample is not a required step in the PCR workflow, but it can save you time by helping to ensure that your downstream processing is more successful. Understanding if you have sufficient nucleic acid in your sample prior to proceeding can help prevent costly delays and wasted effort as you move forward.

 

There are two optical technologies commonly used to quantify nucleic acids: UV-Vis measurement and fluorescence measurement. Learn more about DNA quantification techniques and find the right technology for your sample, workflow, and throughput.

 

For Research Use Only. Not for use in diagnostic procedures.