Novex WedgeWell Tris-Glycine Precast Protein Gels

Load more sample with these precast protein gels

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Today many labs depend on the convenience and consistency provided by commercially available, ready-to-use, precast protein gels. Technological advances in buffer formulations and gel polymerization methods have enabled manufacturers to produce precast gels—even gradient gels—with greater uniformity than ever before. Moreover, the use of precast gels means less exposure to the acrylamide monomer (a known neurotoxin and suspected carcinogen) for the bench scientist.

The next generation of precast protein gels

Based on traditional Laemmli protein electrophoresis, the Invitrogen™ Novex™ WedgeWell™ precast protein gels (Figure 1) include innovations in both chemistry and design that produce:

• Improved shelf life—store gels for up to 12 months at 4°C
• Increased sample capacity—easily load up to 60 μL of sample per well
• Reliable protein detection—obtain exceptional protein band resolution and sharpness
• Fast run conditions—quickly separate proteins using constant voltage in less than 60 minutes
• More flexibility in experimental design—gels can be used to run native or denatured protein samples

Increased sample volume capacity

Traditionally, researchers working with low-abundance proteins or dilute protein samples had to compromise on target detection limits because of small sample volume capacities on protein gels. The Novex WedgeWell precast protein gels offer one of the highest sample loading capacities available in a mini-sized (8 x 8 cm), 1.0 mm thick Tris-glycine gel due to their proprietary wedge-shaped well design (Figure 2). This novel design nearly doubles the sample capacity without the need to increase gel thickness, thus allowing for transfer of larger amounts of protein to western blots and more sensitive detection of low-abundance proteins.

Improved band resolution and flexibility

The modified chemistry of the Novex WedgeWell precast protein gels produces less band distortion and greater band resolution, as compared with classic Laemmli gels used by other suppliers (Figure 3). Additionally, Novex WedgeWell gels do not contain SDS and can therefore be used to run proteins in either native or denatured form, using either the Invitrogen™ Novex™ Tris-Glycine Native Sample Buffer and Running Buffer or the Invitrogen™ Novex™ Tris-Glycine SDS Sample Buffer and Running Buffer.