Complex Gene Synthesis | Thermo Fisher Scientific

Genes and sequences with challenging features such as complex secondary structure, repetitive sequences (direct or indirect), high (>80%) or low (<20%) GC content, or long polypurine/polypyrimidine runs cannot be synthesized using our standard process. For these projects, we have developed a unique proprietary technology that employs specially modified and purified oligonucleotides, and more sophisticated gene assembly methods.

Using this advanced technology, even relatively complex sequences can be synthesized and assembled rapidly and reliably (see Figures 1 and 2). In many cases these projects can be completed and shipped with the same turnaround time as our conventional gene synthesis projects.

For details on our Complex Gene Synthesis service, including a price and delivery estimate, send your sequence for analysis by going to our secure online customer portal.

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Figure 1: Example Sequence With Repetitive Gene Structure

Shown is a dot matrix plot of an example of a gene with areas of repetetive sequence. The nucleotide sequence is plotted on horizontal and vertical axes. Sections with similar sequence (e.g., 9 of 10 identical basepairs) are indicated by a diagonal row of dots.

Figure 2: Example of Sequence with very high GC content

The plot shows the GC content in a 40 bp window centered at the indicated nucleotide position. In this example, ~200 bp of the 5’ end of the sequence has an extremely high GC content.

For Research Use Only. Not for use in diagnostic procedures.

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