Oligo Ordering and Delivery

Synthesis scales refer to the amount of starting material present, not the amount of final product produced. This is the same for all manufacturers of synthetic DNA. When a 50 nmole scale synthesis is specified, approximately 50 nmoles of the first base is added to the DNA synthesizer. For an average 25-mer, at least 25% of this starting material will result in failure sequences, hence it is not possible to produce 50 nmoles of full-length product from a 50 nmole scale synthesis. In addition, further losses occur during processing, transfer of material, and quality control.

Estimated nmole Yield
The table below shows estimated nmole yield for desalted oligos at the various starting synthesis scales. This table converts the OD units into nmole values for an oligo with an equal and even distribution of each base. The actual nmol conversion from OD for a specific oligo may be different based on the composition and distribution of each base within the oligo.

Approximate nmole Ending Yield at Each Starting Synthesis Scale

Minimum Yields
When ordering custom oligos, remember that the scale of synthesis (25 nmol, 50 nmol, 200 nmol, 1 µmol, or 10 µmol) is the starting point for synthesis, not the guaranteed final amount. Oligonecleotide length, sequence, GC content, and coupling efficiencies can vary the yield of nmole amounts post-synthesis. Invitrogen guarantees the total yield of oligonucleotide as a minimum number of OD units. Purification by HPLC, PAGE, and Cartridge will also affect final product yield.

N/A : Not Available
*1　Guaranteed minimum OD yield for 7-10mer is 4.
*2　Guaranteed minimum OD yield not available
*3　Manufactured overseas
*4　Guaranteed minimum OD yield for 66-80mer is 2.
*5　Not available for 66-80mer

Please click here for information of delivery time.

1. “Synthesis scale” means the starting material volume. Final delivery material volume will change by base number and sequence. Please click here for minimum guarantee volume.
2. HPLC can provide up to 65mer.
3. Delivery time is 3weeks because performing in US site.

1. “Synthesis scale” means the starting material volume.
2. Minimum guarantee volume is same as 50n mol scale's one.
3. 7-30mer, you can include mix oligonucleotides.
4. Value oligo can provide non modification and desalted.

• Please contact to order support if you want to order more than 81mer or 11plates.
• Invitrogen can provide desalted or cartridge puritication for plate oligonucleotides.
• Please contact to order support if you want to order HPLC or PAGE purification for plate oligonucleotides.
• You can order the greater than or equal 24 primers.

Dilution buffer : TE, (Option) Sterilized water, etc. Shipping plate format : Deep well plate, (Option) Half well plate or Micro well plate. Quality Control : Trityl monitoring, Capillary electrophoresis, Mass analysis, Absorbance determination(OD260).

1. We are doing sale/synthesis under license from Wakunaga Pharmaceutical Co., Ltd.
2. We cannot take cartridge purification order.
3. If you order HPLC purification, you can choose the protecting group of terminal thiol.
   - Protection group "ON" (-SSR. do not treat deprotection reaction.)
   - Protection group "OFF" (-SH. treat deprotection reaction.)
   If you order desalted or cartridge purification, we can ship only protecting group “ON”.
4. If you request cartridge purification, we can provide up to 55mer.
5. The price of one base is including standard price and additional price.
   ex) 50nmol, Desalted, Deoxyinosine => 60JPY + 1,800JPY = 1,860JPY/modified base
6. We can provide HPLC purification only.
7. We cannot provide the modification of phosphorothioates on 3’terminal by structure issue of oligonucleotide.
   Please choose oligonucleotide the A or G or C or T on 3' terminal.
   If you request phosphorothioates modification on 5’ terminal, please contact to customer service.

If you want to request other modification, please contact to customer service.

(Business Day)

If the standard oligo is internally modified (inosine, uracil) or 5'terminal modified (amino, phosphoric acid, biotin, HEX, TET, FAM, Fluoro), please add one day for delivery.

• *1 HPLC refined products can be selected from thiol without protecting groups (-SH, with deprotection treatment) and with protecting groups (-SSR, without deprotection treatment).
• *1 Desalted and cartridge are only available with protecting groups (-SSR, without deprotection treatment).
• *2 Only 7-55mer※2 Only 7-55mer
• *3 All HPLC purification is available only for 65mer or less.

(Business Day)

1. Delivery schedule is subject to change.
2. This delivery schedule is applied to the order by Web(-20:00), E-mail(-16:00), FAX(-14:00).

For all inquiries, please contact us.
Life Technologies Japan Ltd. 
Order support (Custom primer)
TEL：03-6832-6980
FAX：03-6832-9585
E-mail：jpprimerorder@invitrogen.com

Easy to spot, oligonucleotides are shipped in specially marked envelopes. Rapid, reliable delivery is ensured by overnight express. The table below highlights specific service and delivery time frames. Delivery schedules may be affected by demand fluctuations. We make every effort to deliver your high quality oligonucleotides as quickly as possible.

Tubes Delivery Schedule

* Delivery time will change on delivery area.
* Delivery time is subject to change based on ordered oligos number and modification type.
* If you have any futher questions or concerns, please feel free to contact to customer service.

Plate Delivery Schedule

For plate orders containing disalted unmodified oligos of 50 mer or less.

* If you have any futher questions or concerns, please feel free to contact to customer service.

Ordering Details

Key Features of our Unique Processes

Invitrogen uses a state of the art automated process to increase performance, speed and capacity. This means you get quality Custom Primers quickly and efficiently. Custom DNA Oligos are available as standard deoxynucleotides, modified based, 5’ modified nucleotides (including wide variety of fluorescent dyes, enzyme conjugates), and S-oligos for antisense studies. Five scales and four purity levels are available for DNA oligos in both tube and plate formats.

Our unique process includes 100% in-process trityl monitoring with up to 12 measurements taken for every base addition and additional CE, MS, and OD to verify yield. This comprehensive QC also applies to our Next-Day delivery service. Our oligo offering is complimented by easy online ordering and overnight shipping for rapid, reliable delivery. Consistent oligonucleotide quality is guaranteed and a comprehensive Certificate of Analysis accompanies each order, indicating quality, molecular weight, extinction coefficient, sequence, and melting temperature. No other oligo vendor provides superior performance or better value.

Additional Features of our Process

• Final testing of statistically selected oligos by capillary electrophoresis or by mass spectroscopy to further ensure the quality.
• Certificate of Analysis includes oligo name; scale of synthesis; sequence and length; melting temperature (TM); amount in ODs, µg, and nmole; and molecular weight and extinction coefficient calculated from your exact sequence, including modification.
• Label information on each vial is durably printed on clear transparent stock to allow full view of contents.
• Fluorescent oligos shipped in amber tubes to protect light sensitive oligos. Award winning Technical Services for complete workflow support.
• Shipping in specially designed, easy-to-identify envelopes by express overnight delivery.