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Accelerate Your PCR and qPCR Workflow
Maximize real-time PCR results by pairing PowerTrack SYBR Green Master Mix with either SuperScript IV VILO reverse transcriptase for superior cDNA synthesis or SuperScript IV CellsDirect cDNA synthesis kit to go from mammalian cells all the way to first-strand cDNA synthesis without having to isolate RNA. These best-in-class reagents were designed together to help accelerate your research by offering accurate data quickly.
Did you know your institutional price for these products might be much lower than the list price? Simply log onto your thermofisher.com account to see how much you can save.
25% discount on the workflow bundle
Place SuperScript IV Vilo Master Mix Cat No 11756050 and PowerTrack SYBR Green Master Mix Cat No A46109 in your shopping cart and enter code RJXPT25 to get 25% off*
The Invitrogen SuperScript IV VILO Master Mix is a first-strand cDNA synthesis reaction mix for two-step RT-qPCR. The master mix format elevates the trusted VILO technology (Variable Input, Linear Output) to the next level by combining further optimized buffer conditions with highly processive and thermostable Invitrogen SuperScript IV Reverse Transcriptase (RT). The master mix offers exceptional performance features while maintaining superior linearity across the broadest range of input RNA SuperScript IV VILO Master Mix for reverse transcriptase features:
- Super-efficient–up to 100x higher cDNA yield
- Super-sensitive–Ct values reduced by as many as 8 cycles for RT-qPCR
- Super-robust–transcribes even from degraded or inhibitor-containing RNA samples
- Super-fast–10 min reaction time
The SuperScript IV CellsDirect cDNA Synthesis kit is designed to synthesize first-strand cDNA directly from mammalian cell lysates without requiring the initial isolation of RNA. Lysis and reverse transcription are performed in the same tube. The resulting first-strand cDNA is ready to use in downstream applications including PCR and qPCR. This cDNA synthesis kit has been optimized for small cell samples, ranging from 10,000 cells down to a single cell. The SuperScript IV CellsDirect cDNA Synthesis kit features:
- Time-saving–save up to two hours in overall workflow time
- Streamlined workflow–lysis and RT reaction times reduced and completed in a single tube
- Superior sensitivity–easy detection of low abundant targets
- Direct convenience–no need to isolate RNA and store in your freezer
The PowerTrack SYBR Green Master Mix is a flexible, easy-to-use gene expression master mix for real-time PCR. The PowerTrack SYBR Green Master Mix features:
- Two-color tracking dye system to indicate where pipetting has occurred.
- Broad primer Tm and primer concentration compatibility for flexibility in qPCR reaction setup with minimal optimization.
- Superior specificity and tight reproducibility in Ct values over a broad dynamic range to improve data quality.
- Compatibility with SuperScript IV VILO master mix reverse transcription for fast, reproducible results.
- UNG/dUTP to prevent contamination from carryover PCR products.
- Broad instrument compatibility.
The Invitrogen SuperScript IV VILO Master Mix is a first-strand cDNA synthesis reaction mix for two-step RT-qPCR. The master mix format elevates the trusted VILO technology (Variable Input, Linear Output) to the next level by combining further optimized buffer conditions with highly processive and thermostable Invitrogen SuperScript IV Reverse Transcriptase (RT). The master mix offers exceptional performance features while maintaining superior linearity across the broadest range of input RNA SuperScript IV VILO Master Mix for reverse transcriptase features:
- Super-efficient–up to 100x higher cDNA yield
- Super-sensitive–Ct values reduced by as many as 8 cycles for RT-qPCR
- Super-robust–transcribes even from degraded or inhibitor-containing RNA samples
- Super-fast–10 min reaction time
The SuperScript IV CellsDirect cDNA Synthesis kit is designed to synthesize first-strand cDNA directly from mammalian cell lysates without requiring the initial isolation of RNA. Lysis and reverse transcription are performed in the same tube. The resulting first-strand cDNA is ready to use in downstream applications including PCR and qPCR. This cDNA synthesis kit has been optimized for small cell samples, ranging from 10,000 cells down to a single cell. The SuperScript IV CellsDirect cDNA Synthesis kit features:
- Time-saving–save up to two hours in overall workflow time
- Streamlined workflow–lysis and RT reaction times reduced and completed in a single tube
- Superior sensitivity–easy detection of low abundant targets
- Direct convenience–no need to isolate RNA and store in your freezer
The PowerTrack SYBR Green Master Mix is a flexible, easy-to-use gene expression master mix for real-time PCR. The PowerTrack SYBR Green Master Mix features:
- Two-color tracking dye system to indicate where pipetting has occurred.
- Broad primer Tm and primer concentration compatibility for flexibility in qPCR reaction setup with minimal optimization.
- Superior specificity and tight reproducibility in Ct values over a broad dynamic range to improve data quality.
- Compatibility with SuperScript IV VILO master mix reverse transcription for fast, reproducible results.
- UNG/dUTP to prevent contamination from carryover PCR products.
- Broad instrument compatibility.
*No purchase necessary. Available to life sciences professionals 21 years and older in select countries globally. Offer will apply to sample requests until promotional supplies are depleted. Sample size: 1 mL for qPCR master mix, 10 reactions for RT. Limit one sample per laboratory. Offer void where prohibited by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply. View the privacy policy.
*Promotion is only valid in the US (excluding Puerto Rico) and Canada on the purchase of a PowerTrack SYBR Green Master Mix (5 mL) and 50 reactions of SuperScript IV VILO Master Mix in a single purchase order. Promotional code RJXPT25 must be presented at time of order. Promotion is valid on orders received by Life Technologies no later than June 30, 2021, or until promotional supplies are depleted, whichever comes first. This promotion cannot be combined with any other promotions or discounts. There is no limit on the number of times the promotion code can be used. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency/institutional policy. Other restrictions may apply.
For Research Use Only. Not for use in diagnostic procedures.