Protein Expression Essentials

We offer a range of protein expression systems and tools that are easy to use, designed to deliver high protein yields for a wide variety of downstream applications, and allow you to achieve results quickly. Creating your gene, purifying your plasmid, and selecting suitable protein expression products are simplified with help from our selection tools and educational videos.

When selecting a host system for expressing protein there are many factors to consider. These include: post translational modifications, equipment availability, and time constraints. We have mapped out the details for each host system, that along with information for applicable products the we offer, to allow you to make the most appropriate choice based on your needs and budget.

	Do you need modifications?			How quickly do you require protein?			What capabilities do you have to support expression (lab equipment & resources)?
	None	Some	Complete	Days	Weeks	Months	None	Some	Many
Mammalian
Bacteria
Yeast
Insect

Get more detail about comparing protein expression systems

Other questions to consider when choosing an expression system

Equipment

What equipment is available for use?

Type of protein

Is your protein endogenously expressed in bacterial or yeast cells?
Do you know that it’s insoluble in a bacterial expression system?
Do you need native posttranslation modifications?
Do you want some level of eukaryotic posttranslational modifications?

Desired preparation time

One week or less (bacterial, transient mammalian)
One month or less (in addition to bacterial and transient mammalian, yeast & insect systems can be used)
More than one month (in addition to the systems above, you can use stable mammalian systems)

Mammalian expression

Vector selection

Cloning

Transform in E. coli

Plasmid purification

Transfection

Expression

Purification

Analysis

Determined by promoter and terminator

Overview	Choosing expression	Additional information
Protein expression workflow Selecting a host system	Mammalian Bacteria Yeast Insect	Education & Videos Promotions

On this page

Overview

Choosing expression

Additional information

Equipment

Type of protein

Desired preparation time

How-to videos

"Meet the Inventor" Series