Dialysis Products Selection Guide

Thermo Scientific™ SnakeSkin™ Dialysis Tubing was developed to simplify large-volume sample dialysis and does not require presoaking or boiling the membrane prior to use. SnakeSkin Dialysis Tubing is regenerated cellulose dialysis tubing that is pleated (compressed) into a hollow stick and can be used for a wide range of sample volumes. The hydrated tubing will hold ~3.7 mL of sample per centimeter of length.

The pleated format of SnakeSkin Dialysis Tubing makes it easy to open and ready to use; simply pull out the required length of tubing, cut it off and apply a closure. The recommended closures, SnakeSkin Dialysis Tubing Clips, are sold separately. Alternatively, SnakeSkin Dialysis Tubing can also be closed with knots after prewetting the tubing in water or buffer.

SnakeSkin Dialysis Tubing is available in three molecular weight cutoff (MWCO) specifications: 3.5K, 7K and 10K.

Thermo Scientific™ Slide-A-Lyzer™ Dialysis Flasks are for sample volumes from 150 to 250 mL. These devices accomplish typical buffer exchange in 8 to 24 hours and are available in four molecular-weight cutoff (MWCO) varieties: 2K, 3.5K, 10K, and 20K.

The flask-style dialysis devices are very easy to use. Add and remove sample using a serological pipette or by directly pouring the sample through a wide screw-cap opening at the top of the flask. No knots or clips are needed to seal the units.

Slide-A-Lyzer Dialysis Flasks are manufactured using clean room conditions and have low-protein binding regenerated cellulose membranes to help ensure maximum sample recovery and sample purity.

Thermo Scientific™ Slide-A-Lyzer™ Dialysis Cassettes (original and G2 models) facilitate rapid and trouble-free dialysis for sample volumes from 100 µL to 70 mL. The cassette design helps maximize surface-area per sample-volume ratio and allows excellent sample recoveries. Unlike standard flat tubing, these innovative cassettes do not require knots or clips that can lead to leaking and sample loss. Instead, Slide-A-Lyzer Dialysis Cassettes are constructed of dialysis membranes separated by an inert gasket and sandwiched between two halves of a translucent plastic case that is sonically welded together.

• Original-style Dialysis Cassettes:
  Designed with an inner chamber that is hermetically sealed, allowing the sample to be accessed by inserting a syringe needle through the inlet port.
   
• 2nd-Generation (G2) Dialysis Cassettes:
  Designed with two additional features: (1) they have needle-accessible ports plus a unique pipette-accessible port, and (2) they are self-floating.


• 3rd-Generation Slide-A-Lyzer Cassettes: Designed with a much more compact form factor, higher volume options, redesigned cap and up to 68% less plastic waste than Slide-A-Lyzer G2.

Thermo Scientific™ Slide-A-Lyzer™ MINI Dialysis Devices are disposable polypropylene cups with integrated, low-binding membranes for convenient, high-performance dialysis of 10 µL to 2 mL samples. The MINI devices are designed to allow easy sample addition and removal using a standard laboratory pipette. They are effective and convenient for accomplishing typical sample desalting or buffer exchange in 4 to 8 hours. Unlike other small sample separation devices, there is no equipment to assemble, no need for syringe adapters and no laborious steps necessary to manipulate the small volume sample. The regenerated cellulose membrane is compatible with a number of common chemicals and buffers.

Slide-A-Lyzer MINI Dialysis Devices are available in three sizes (0.1, 0.5 and 2 mL capacities) with five molecular weight cut-off (MWCO) specifications: 2K, 3.5K, 7K, 10K, and 20K.

Thermo Scientific™ Pierce™ 96-well Microdialysis Plates are automation-compatible, microplate dialysis devices (3.5K or 10K MWCO) for rapidly processing 1 to 96 samples with volumes 10 to 100 µL.

Each sample processed with the Pierce 96-well Microdialysis Dialysis Plate is dialyzed inside a single, removable dialysis device created by two regenerated cellulose membranes separated by ~2 mm. The short diffusion distance and large surface area facilitates rapid dialysis with the removal of salts and small molecules often completed in 2 to 4 hours. Sample recovery is performed using standard laboratory micropipettes. Individual microdialysis devices can also be used with 2 mL microcentrifuge tubes for processing single samples. The available plate seals are made of high-quality polypropylene and help protect samples from contamination and evaporation, especially during long-term storage.

The Thermo Scientific™ RED Device for rapid equilibrium dialysis was developed in association with the pharmaceutical industry to accelerate lead optimization and reduce attrition rate. The RED System consists of disposable tube inserts for use with either a reusable PTFE or disposable high-density polypropylene base plates. The unique design of the base plates enables compatibility with automated liquid handling systems while the large dialysis surface area of the tube inserts helps accelerate equilibrium establishment.

Like the RED Device, the Competition Rapid Equilibrium Dialysis System was developed with accelerated lead optimization as a priority. The Competition RED Device (C-RED) consists of two-chambered disposable tube inserts and a reusable PTFE base plate. Competition equilibrium dialysis is performed within enlarged wells on the base plate that can accommodate 2, 3, 4, or 8 dual chamber inserts enabling a defined space for up to 15 samples and 1 open chamber to access the dialysate. The Competition Rapid Equilibrium Dialysis System is also compatible with automated liquid handling systems and has the same high performance as the RED Device.