Watch demos for each step of the Ion Torrent next-generation sequencing workflow. With the Ion GeneStudio S5 Series, Ion Chef System, and Ion AmpliSeq technology, you can go from formalin-fixed, paraffin-embedded (FFPE) sample to data in as little as 24 hours, with less than 1 hour of hands-on time.

Step 1

Extract DNA from FFPE tissues in a simple one-tube 2-step workflow using the Ion AmpliSeq Direct FFPE DNA Kit

Total hands-on time: 10 minutes

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Answer more genetic queries from your FFPE tissue samples using the Ion AmpliSeq Direct FFPE DNA. Maximize recovery from every precious FFPE sample in only 30 minutes, with no deparaffinization or spin columns required. Achieve targeted sequencing success from as little as 1 ng input DNA.

Watch the video to learn how to quickly prepare DNA from samples with a limited amount of DNA, using this simple and fast method. 

Step 2

Find out how Ion AmpliSeq chemistry works and then design your own panel on ampliseq.com

 

Leveraging over ten years in PCR assay design and preamplification methods developed for our Applied Biosystems platforms, Ion AmpliSeq targeted enrichment offers a vast improvement over early hybridization-based approaches, and delivers a unique, highly multiplexed PCR-based workflow with clear specificity and uniformity benefits. Additionally, extremely low DNA input amounts can be amplified through the PCR process: as low as 1 ng. To tailor this technology to your research, start with the Ion AmpliSeq Designer, a free and intuitive design tool that enables researchers to design custom gene panels for targeted sequencing studies. These panels can be used to investigate the presence of specific SNPs, indels, and gene fusions faster, with less cost and with far less DNA input — as little as 1 ng of DNA — compared to other genetic analysis approaches.

Step 3

Library and template preparation with Ion AmpliSeq libraries and the Ion Chef System

Total hands-on time: 30 minutes

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Paired with Ion Torrent next-generation sequencing platforms (Ion PGM, Ion PGM Dx—assay development mode, Ion Proton, and Ion GeneStudio S5 Series), the Ion Chef System automates Ion AmpliSeq library preparation, template generation, and chip loading with push-button simplicity—enabling a DNA-to-data targeted sequencing workflow with as little as 45 minutes of hands-on time.

Watch the video to learn the quick steps involved in setting up the Ion Chef System for automated library preparation and template preparation. The final loaded chips can then be directly placed on the Ion S5 System for sequencing.

Step 4

Sequencing with the Ion GeneStudio
S5 Series

Total hands-on time: <15 minutes

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In this video, you will see how simple the Ion GeneStudio S5 Series is to set up. First, the video walks through initialization, a quick 30-minute process that gets the system ready for sequencing. Then, you just simply need to swap out the old chip used for initialization with the chip containing your samples, loaded during the Ion Chef System run.

And as far as speed is concerned, sequencing on the Ion GeneStudio S5 Series is fast—allowing you to complete a run in as little as two hours, thanks to the semiconductor sequencing technology.

Step 5

Data analysis with Ion Reporter Software

Test drive Ion Reporter Software

Analysis of next-generation sequencing data is always perceived as being difficult and complicated. Ion Reporter Software provides an optimized suite of simple tools that streamline the Ion GeneStudio S5 Series data analysis steps, so you can focus on finding relevant variants for your research. From human variant detection (SNPs, indels, gene fusions, tumor-normal analysis, trio analysis, and more) to assessing microbial diversity, NGS data analysis has become easy with Ion Reporter Software.

Step 1

Extract DNA from FFPE tissues in a simple one-tube 2-step workflow using the Ion AmpliSeq Direct FFPE DNA Kit

Total hands-on time: 10 minutes

Order now

Answer more genetic queries from your FFPE tissue samples using the Ion AmpliSeq Direct FFPE DNA. Maximize recovery from every precious FFPE sample in only 30 minutes, with no deparaffinization or spin columns required. Achieve targeted sequencing success from as little as 1 ng input DNA.

Watch the video to learn how to quickly prepare DNA from samples with a limited amount of DNA, using this simple and fast method. 

Step 2

Find out how Ion AmpliSeq chemistry works and then design your own panel on ampliseq.com

 

Leveraging over ten years in PCR assay design and preamplification methods developed for our Applied Biosystems platforms, Ion AmpliSeq targeted enrichment offers a vast improvement over early hybridization-based approaches, and delivers a unique, highly multiplexed PCR-based workflow with clear specificity and uniformity benefits. Additionally, extremely low DNA input amounts can be amplified through the PCR process: as low as 1 ng. To tailor this technology to your research, start with the Ion AmpliSeq Designer, a free and intuitive design tool that enables researchers to design custom gene panels for targeted sequencing studies. These panels can be used to investigate the presence of specific SNPs, indels, and gene fusions faster, with less cost and with far less DNA input — as little as 1 ng of DNA — compared to other genetic analysis approaches.

Step 3

Library and template preparation with Ion AmpliSeq libraries and the Ion Chef System

Total hands-on time: 30 minutes

Get a quote

Paired with Ion Torrent next-generation sequencing platforms (Ion PGM, Ion PGM Dx—assay development mode, Ion Proton, and Ion GeneStudio S5 Series), the Ion Chef System automates Ion AmpliSeq library preparation, template generation, and chip loading with push-button simplicity—enabling a DNA-to-data targeted sequencing workflow with as little as 45 minutes of hands-on time.

Watch the video to learn the quick steps involved in setting up the Ion Chef System for automated library preparation and template preparation. The final loaded chips can then be directly placed on the Ion S5 System for sequencing.

Step 4

Sequencing with the Ion GeneStudio
S5 Series

Total hands-on time: <15 minutes

Get a quote

In this video, you will see how simple the Ion GeneStudio S5 Series is to set up. First, the video walks through initialization, a quick 30-minute process that gets the system ready for sequencing. Then, you just simply need to swap out the old chip used for initialization with the chip containing your samples, loaded during the Ion Chef System run.

And as far as speed is concerned, sequencing on the Ion GeneStudio S5 Series is fast—allowing you to complete a run in as little as two hours, thanks to the semiconductor sequencing technology.

Step 5

Data analysis with Ion Reporter Software

Test drive Ion Reporter Software

Analysis of next-generation sequencing data is always perceived as being difficult and complicated. Ion Reporter Software provides an optimized suite of simple tools that streamline the Ion GeneStudio S5 Series data analysis steps, so you can focus on finding relevant variants for your research. From human variant detection (SNPs, indels, gene fusions, tumor-normal analysis, trio analysis, and more) to assessing microbial diversity, NGS data analysis has become easy with Ion Reporter Software.

For Research Use Only. Not for use in diagnostic procedures.