Primer Designer Tool for PCR & Sanger Sequencing

Streamline your workflow with our primer design tool

From design to synthesis, quality primers are vital to successful results. Use our online Applied Biosystems™ Primer Designer™ Tool to search for the right PCR/Sanger sequencing primer pair from a database of ~650,000 predesigned primer pairs for resequencing the human exome and human mitochondrial genome. Choose from different amplicon lengths to accommodate various research applications and biological sample types.

• Our primers are free of known SNPs and primer-dimers, highly target-specific and used under universal PCR conditions 
• Full primer coverage for Ion Torrent™ AmpliSeq™ Exome Panel and Ion Torrent™ AmpliSeq™ Cancer Hotspot Panel v.2 Sanger confirmation workflow
• Flexible primer configuration to meet your research needs: primers can be ordered unmodified, M13-tailed, HPLC-purified or desalted
• All the primers have been checked by mass spectrometry and have passed stringent bioinformatics metrics; lab bench validation tests have shown >95% success rate

(File must be less than 500 kb in size and contain less than 1000 unique line-items.)