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Fragment Analysis Support
Explore the “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems related to fragment analysis. Browse through the “Guides and Tools” section to access comprehensive product-related support resources.
Getting Started
- What reagents do I need to start a fragment analysis project?
- What is the largest fragment size or resolution that can be detected on a capillary electrophoresis instrument?
- Which fluorescent dyes can I use for primer labeling prior to PCR amplification for fragment analysis?
- Can I multiplex my PCR reaction for loading onto a capillary electrophoresis instrument?
- Which size standard should I use for fragment analysis?
Troubleshooting
- How do I begin troubleshooting my fragment analysis experiment?
- What is the difference between pooling PCR products and multiplex PCR?
- I am performing fragment analysis and my sample signal is low. Why?
- I am able to visualize PCR products on an agarose gel but not on the capillary electrophoresis (CE) instrument. Why?
- My fragment analysis data is offscale. What can I do to prevent this?
Guides and Tools
Search our database for Product Documents: Manuals, Product Literature, Citations and References
Search our database for Quality and Safety Documents: SDS, COA
- Overview: Fragment Analysis Applications for Your Genetic Analyzer
- Overview:Fragment Analysis Fundamentals
- Overview:Quantitative Fluorescence PCR (qf-pcr)
- Overview: Amplified Fragment Length Polymorphism-AFLP® Analysis
- Overview: Restriction Fragment Length Polymorphisms (RFLP) Analysis
- Overview: Single-Strand Conformation Polymorphism (SSCP) Analysis
- Overview: Bacterial Artificial Chromosome (BAC) Fingerprinting
- Overview:SNP Genotyping by Fragment Analysis
- Overview: Single-Strand Conformation Polymorphism (SSCP) Analysis
- Citations: Fragment Analysis Publications and Literature
- Video: Fragment Analysis Overview
- Video: Seq It Out Video Series
- Training: GeneMapper™ Software Theory and Software Demonstrations (2 hrs) (for US customers)
- Browse our site for: Training, Events and Webinars
- Sanger Sequencing and Fragment Analysis Software Trials: download a trial version of Applied Biosystems’ commercially available software
- Applied Biosystems™ Genetic Analyzer Freeware and Patches : download freeware and patches for your Capillary Electrophoresis instrument
- Thermo Fisher Cloud: a secure place to store, analyze, and share data
- Capillary Electrophoresis Guides and Catalog Download
Custom Services
Applied Biosystems
Custom Primers
Order oligonucleotides that are custom manufactured to your specifications, including 5’ fluorescent labeled primers and primer pairs for use in CE (Sanger) sequencing.
Compliance, Validation, and Calibration Services
Get compliance and validation services for Applied Biosystems and Ion Torrent instruments including next-generation and Sanger Sequencing platforms as well as their connected computers and electronic records.
Instrument Service Plans and Service Options
Get instrument service plans for Applied Biosystems and Ion Torrent instruments including next-generation and Sanger Sequencing platforms
For Research Use Only. Not for use in diagnostic procedures.