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GeneRuler DNA Ladders: clear, crisp bands
Thermo Scientific GeneRuler DNA Ladders contain mixtures of individual chromatography-purified DNA fragments, designed to deliver precision and ease-of-use for everyday electrophoresis.
Benefits of GeneRuler DNA Ladders
Benefit #1: Quality and purity of GeneRuler DNA Ladders
The chromatography-purified DNA fragments in GeneRuler ladders produce high quality reference bands. In routine gel electrophoresis, GeneRuler DNA ladders produce clean, crisp bands in the gel with recognizable patterns (Figure 1).
In capillary electrophoresis, GeneRuler DNA ladders produces sharp peaks (Figure 2). The mass of the DNA fragments in GeneRuler ladders are verified spectrophotometrically; this allows for the estimation of the mass of sample DNA with greater accuracy.
Click image to enlarge
Figure 1. A comparison of reference bands produced by GeneRuler DNA ladder and other commercially available DNA ladders. GeneRuler DNA ladders produce clean and crisp reference bands, with an easy-to-remember pattern. The volume of DNA ladders added to each lane was in accordance with manufacturer recommendation (A) Lane 1: GeneRuler 1 kb DNA Ladder, ready-to-use; lane 2: GeneRuler 1 kb Plus DNA Ladder, ready-to-use; lane 3: Sigma-Aldrich DirectLoad™ Wide Range DNA Marker; lane 4: NEB Quick-Load™ 2-Log DNA Ladder (0.1–10.0 kb); lane 5: NEB Quick-Load™ 1 kb DNA Ladder. (B) Lane 1: GeneRuler 100 bp DNA Ladder, ready-to-use; lane 2: GeneRuler 100 bp Plus DNA Ladder, ready-to-use; lane 3: Sigma-Aldrich DirectLoad™ PCR 100 bp Low Ladder; lane 4: NEB Quick-Load™ Purple 100 bp DNA Ladder.
Click image to enlarge
Figure 2. A comparison of peak widths produced by GeneRuler DNA ladder and other commercially available DNA ladders. GeneRuler DNA ladder produces sharp peaks compared to broad and double peaks produced by competitor products. (A) Thermo Scientific GeneRuler 100 bp DNA Ladder (red) and Promega 100 bp DNA Ladder (blue). (B) GeneRuler 100 bp DNA Ladder (red) and Sigma-Aldrich DirectLoad™ PCR 100 bp Low Ladder (blue).
Benefit #2: Easy tracking of DNA migration with TriTrack Dye premixed in GeneRuler DNA Ladders
The TriTrack DNA Loading Buffer, 6X contains three dyes (bromophenol blue, xylene cyanol FF, and orange G) for visual tracking of DNA migration during electrophoresis. These dyes do not interfere with visualization.
Ready-to-use DNA ladders premixed with the TriTrack Loading Buffer are stable at room temperature up to six months.
Benefit #3: Go green with GeneRuler DNA Ladders
GeneRuler DNA ladders are earth-friendly products. Organic solvents are not used in production of Thermo Scientific DNA ladders, which helps reduce environmental waste from manufacturing processes. Furthermore, GeneRuler ladders are stable at ambient temperature and shipped in non-insulated shipping boxes, which generate less waste compared to insulated boxes.
GeneRuler family of DNA ladders
GeneRuler DNA ladders can be ideal for DNA sizing and approximate quantification of a wide range of double-stranded DNA fragments ranging between 10 bp and 48.5 kb.
Click image to enlarge
GeneRuler DNA ladders are available in two formats:
- Dye-free ladders
- Ready-to-use (RTU) ladders, which are premixed with 6X TriTrack DNA Loading Dye
GeneRuler DNA Ladder selection guide
GeneRuler DNA Ladders are routinely used in the molecular biology techniques such as DNA cloning, PCR, and reverse transcription. Choose the DNA ladder that best fits your needs.
| Product name | GeneRuler Ultra Low Range DNA Ladder | GeneRuler Low Range DNA Ladder | GeneRuler 50 bp DNA Ladder | GeneRuler 100 bp DNA Ladder | GeneRuler 100 bp Plus DNA Ladder |
|---|---|---|---|---|---|
| Ready-to-use format | SM1213 | SM1193 | SM0373 | SM0243 SM0244 | SM0323 SM0324 |
| Colorless DNA ladders (dye not premixed) | SM1211 SM1212 | SM1191 SM1192 | SM0371 SM0372 | SM0241 SM0242 | SM0321 SM0322 |
| Base pairs (bp) | 10–300 | 25–700 | 50–1,000 | 100–1,000 | 100–3,000 |
 |  |  |  |  |
* Bands represent each of the DNA fragments. The dark bands represent reference bands.
| Product name | GeneRuler 1 kb DNA Ladder | GeneRuler 1 kb Plus DNA Ladder | GeneRuler Express DNA Ladder | GeneRuler DNA Ladder Mix | GeneRuler High Range DNA Ladder |
|---|---|---|---|---|---|
| Ready-to-use format | SM0314 SM0313 | SM1333 SM1334 | SM1553 | SM0334 SM0333 | SM1353 |
| Colorless DNA ladders (dye not premixed) | SM0311 SM0312 | SM1331 SM1332 | SM1551 SM1552 | SM0331 SM0332 | SM1351 SM1352 |
| Base pairs (bp) | 250–10,000 | 75–20,000 | 100–5,000 | 100–10,000 | |
 |  |  |  |  |
* Bands represent each of the DNA fragments. The dark bands represent reference bands.
| Product name | GeneRuler Ultra Low Range DNA Ladder | GeneRuler Low Range DNA Ladder | GeneRuler 50 bp DNA Ladder | GeneRuler 100 bp DNA Ladder | GeneRuler 100 bp Plus DNA Ladder |
|---|---|---|---|---|---|
| Ready-to-use format | SM1213 | SM1193 | SM0373 | SM0243 SM0244 | SM0323 SM0324 |
| Colorless DNA ladders (dye not premixed) | SM1211 SM1212 | SM1191 SM1192 | SM0371 SM0372 | SM0241 SM0242 | SM0321 SM0322 |
| Base pairs (bp) | 10–300 | 25–700 | 50–1,000 | 100–1,000 | 100–3,000 |
| | | | |
* Bands represent each of the DNA fragments. The dark bands represent reference bands.
| Product name | GeneRuler 1 kb DNA Ladder | GeneRuler 1 kb Plus DNA Ladder | GeneRuler Express DNA Ladder | GeneRuler DNA Ladder Mix | GeneRuler High Range DNA Ladder |
|---|---|---|---|---|---|
| Ready-to-use format | SM0314 SM0313 | SM1333 SM1334 | SM1553 | SM0334 SM0333 | SM1353 |
| Colorless DNA ladders (dye not premixed) | SM0311 SM0312 | SM1331 SM1332 | SM1551 SM1552 | SM0331 SM0332 | SM1351 SM1352 |
| Base pairs (bp) | 250–10,000 | 75–20,000 | 100–5,000 | 100–10,000 | |
| | | | |
* Bands represent each of the DNA fragments. The dark bands represent reference bands.
GeneRuler DNA Ladder literature/citations
Resources for GeneRuler DNA Ladders
References
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