Properties of RNA polymerases for in vitro transcription, RNA labeling, synthesis of antisense RNA and siRNA, studies of RNA splicing and secondary structure

PolymeraseApplicationsPromoter sequence*Concentration**
T7 RNA Polymerase
  • In vitro transcription
  • Synthesis of unlabeled and labeled RNA
T7 RNA Polymerase
T3 RNA PolymeraseT3 RNA Polymerase
SP6 RNA PolymeraseSP6 RNA Polymerase

* Position +1 indicates the first nucleotide incorporated into RNA during transcription. Only bases at positions +1 through +3 are critical for transcription, and they must be a G and 2 purine bases, respectively.
** Standard concentration is 20 U/µL; HC = high concentration (> 100 U/µL)

Additional polymerases