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EVOS M5000 Imaging System
The EVOS M5000 microscope is a workhorse for cell imaging labs across a broad range of applications and imaging requirements with minimal requirement for personal contact with the instrument.
The EVOS M5000 Imaging System offers you these important advantages:
- No ocular, on-screen display, minimal handling and many more safety features
- Easy installation; no maintenance, assembly, alignment, or calibration
- User defined four-color fluorescence, transmitted light and color applications
- Autofocus, Z-stack capability, time-lapse imaging, live-cell imaging with onstage incubator and single-click multi-channel capture
- On-board software for fast acquisition and analysis, including confluency measurements and automated cell counting
- Access to Thermo Fisher Cloud imaging app
- Semi-automatic, less manual contact
The EVOS XL, EVOS FL Color and EVOS FL imaging systems (Cat. Nos. AME3300, AMEFC4300 and AMF4300) have been discontinued and replaced by a no personal contact-based system: the EVOS M5000 Imaging System.
Imaging made more safe with EVOS imaging systems
- On-screen display (no oculars)
- Automated controls, minimal handling
- Swift decontaminations
- Fits in biosafety cabinets
- Easy routine maintenance
Learn what you can now do with an EVOS M5000 Imager
Learn about software capability with M5000 Imaging System
Powerful, intuitive software
All EVOS microscopes come with onboard software that allows you to view, capture, and process images quickly and easily. Featuring a user interface and workflow designed by biologists for biologists, the software gives you full control over your fluorescence, brightfield, color brightfield, and phase contract images.
On the M7000 and M5000, the onboard software offers pinpoint operational control and powerful image processing tools such as cell counting, confluence, Z-stack visualization, and time-lapse movies. These tools operate intuitively with minimal training, aided by onboard SmartStart guides. Autofocus and other automated tools save time and effort. The M7000 adds image tiling and stitching as well as fully automated capabilities for image acquisition and processing when scanning tissue slices or acquiring data from multiwell plate vessels. The ability to analyze images in batch mode allows you to seamlessly process large quantities of data.
Click image to enlarge
Fast, robust autofocus
The autofocus algorithm on the EVOS M7000 and M5000 is fast and efficient in any channel. For automated routines with the M7000, you can choose the interval at which autofocus is used in an area scan or multiwell plate acquisition.
Click image to enlarge
Cell confluence app
Automatically measure and document the percent confluence of your cultured cells, allowing consistent passaging and increasing reproducibility of downstream cell-based assays. Use it for a single field or in batch mode for multiple fields and wells.
Click image to enlarge
Automatic cell counting
Advanced tools let you identify and count fluorescent objects while gating out undesired objects based on size, brightness, and circularity. Apply to a single field or to a multiwell plate using batch analysis.
Click image to enlarge
Measure transfection efficiency in cultured cells
Automatically calculate transfection efficiency based on a user-adjustable threshold of positive pixels in a transmitted light or fluorescence image. Apply to a single field or to multiple fields using batch analysis.
Images captured using the EVOS M5000 Imaging System
Click image to enlarge
Figure 1. Proliferation (EdU labeled) with Movat’s Pentachrome staining in FFPE 8 μm rat intestine section. A transverse section through the crypts shows proliferating cell locally abundant near the base of the crypts and diminishing with distance. Proliferating cells (brown) signal is visible against Movat’s Pentachrome staining consisting of Verhoeff-Van Gieson’s staining of elastin (black) and nuclei (purplish gray), alcoholic saffron staining of collagen (yellow), alcian blue staining of mucins marking the goblet cells (blue), and crocein scarlet–acid fuchsin staining of muscle and red blood cells (red). Image was acquired with a 10X objective using the RGB-mode illumination on an EVOS M5000 microscope.
Click image to enlarge
Figure 2. Differentiated mouse embryonic stem cells. The image shows mouse embryonic stem cells that have spontaneously differentiated into the three germ layers. The ectoderm was stained using anti-TUJ1 antibody, and the mesoderm was stained using anti-SMA antibody. Staining was done using secondary antibodies labeled with Alexa Fluor 488 and 555, respectively, and nuclei were stained using DAPI. Images were acquired at 10X using an EVOS M5000 microscope.
Click image to enlarge
Figure 3. Z-stack of honey bee claw imaged with EVOS M5000. MIP projection of an 11-slice image Z-stack of a honey bee claw captured using the EVOS 5000 microscope in color mode with a 10x apo objective.
Click image to enlarge
Figure 4. Transgenic fluorescent zebrafish (Fli1–enhanced Green Fluorescent Protein) including chorion, 24 hours post fertilization. Embryos were fixed in 4% paraformaldehyde and then imaged in PBS using the EVOS M5000 microscope with a 4x phase objective. Phase image including GFP overlay is shown.
EVOS M5000 – 3D deconvolution and analysis. HeLa Cells were labeled with ViewRNA probes (FITC against B2M and Cy5 against RPL13). Following counter-staining with DAPI, Z-stacks were acquired on an EVOS M5000 microscope with a 100x oil immersion objective. Image stacks were deconvolved using the 3D deconvolution module in Celleste 5.0 Image Analysis software. The animation was also generated in Celleste 5.0 following creation of iso surfaces and clipping planes.
EVOS M5000 Imaging System Specifications
| Optics | Infinity corrected optical system; RMS threaded objectives with 45 mm parfocal distance |
|---|---|
| Illumination | Adjustable intensity LED (>50,000 hour life per light cube); broad selection of patented standard and specialty light cubes |
| Light cubes (not included) | Commonly used light cubes include (Ex/Em):
|
| Contrast methods | Epifluorescence, transmitted light (brightfield and phase contrast) and RGB color illumination |
| Objective turret | 5 position; front mounted control |
| Objectives (not included) | Wide selection of high quality long working distance and coverslip corrected objectives |
| Condenser | 60-mm long working distance condenser; 4-position turret with a clear aperture and 3 phase annuli |
| Focus mechanism | Automated focus with sub micron (0.150 um) resolution (single step accuracy) |
| Mechanical focus wheel with single knob for coarse and fine focus | |
| Stage | Mechanical X/Y stage; travel range 120 mm x 80 mm with sub micron resolution, drop in inserts to receive vessel holders |
| LCD display | 18.5" articulated LCD color monitor with 1920x1080 pixel resolution |
| Camera | High-sensitivity 3.2 MP (2048 x 1536) monochrome CMOS sensor with 3.45 µm pixel resolution |
| Computer | Embedded PC with 4 GB RAM |
| Captured images | 16 bit monochrome TIFF or PNG (12 bit dynamic range); 8 bit color TIFF, PNG JPG and BMP |
| Storage | 10 Gb SSD |
| 16 Gb USB 3.0 memory stick | |
| Output ports | Power, 4 USB 2.0 ports, 1 USB 3.0 port, 1 Display Port, 1 RJ45 network jack |
| Networking capability | Connection through Windows/SMB network via an Ethernet cable connection |
| USB 3.0 WiFi dongle | |
| Power supply | Universal Power Supply (12V, 5A) with Type B (North America) power cord* |
| Dimensions (W x H x D) | 18 x 23 x 18 inches |
| Weight | 36 lb |
| * Note that one (1) country-specific power cord must be ordered separately in regions not using the Type B (North America) power plug: AMEP4646 EVOS Power Cord, Type C (Europe) AMEP4708 EVOS Power Cord, Type D (India/South Africa) AMEP4645 EVOS Power Cord, Type G (United Kingdom) AMEP4649 EVOS Power Cord, Type H (Israel) AMEP4647 EVOS Power Cord, Type I (Australia) AMEP4782 Power Cord, Type I (China, CCC certified) AMEP4786 Power Cord Type J (Switzerland) AMEP4648 EVOS Power Cord, Type L (Italy) | |
Stem cell biology: EVOS M5000 microscope at the cutting edge research
Cardiac repair and heart failure: EVOS M5000 microscope at the cutting-edge research
Liver regeneration: EVOS M5000 microscope at the cutting-edge research
Powerful, intuitive software
All EVOS microscopes come with onboard software that allows you to view, capture, and process images quickly and easily. Featuring a user interface and workflow designed by biologists for biologists, the software gives you full control over your fluorescence, brightfield, color brightfield, and phase contract images.
On the M7000 and M5000, the onboard software offers pinpoint operational control and powerful image processing tools such as cell counting, confluence, Z-stack visualization, and time-lapse movies. These tools operate intuitively with minimal training, aided by onboard SmartStart guides. Autofocus and other automated tools save time and effort. The M7000 adds image tiling and stitching as well as fully automated capabilities for image acquisition and processing when scanning tissue slices or acquiring data from multiwell plate vessels. The ability to analyze images in batch mode allows you to seamlessly process large quantities of data.
Click image to enlarge
Fast, robust autofocus
The autofocus algorithm on the EVOS M7000 and M5000 is fast and efficient in any channel. For automated routines with the M7000, you can choose the interval at which autofocus is used in an area scan or multiwell plate acquisition.
Click image to enlarge
Cell confluence app
Automatically measure and document the percent confluence of your cultured cells, allowing consistent passaging and increasing reproducibility of downstream cell-based assays. Use it for a single field or in batch mode for multiple fields and wells.
Click image to enlarge
Automatic cell counting
Advanced tools let you identify and count fluorescent objects while gating out undesired objects based on size, brightness, and circularity. Apply to a single field or to a multiwell plate using batch analysis.
Click image to enlarge
Measure transfection efficiency in cultured cells
Automatically calculate transfection efficiency based on a user-adjustable threshold of positive pixels in a transmitted light or fluorescence image. Apply to a single field or to multiple fields using batch analysis.
Images captured using the EVOS M5000 Imaging System
Click image to enlarge
Figure 1. Proliferation (EdU labeled) with Movat’s Pentachrome staining in FFPE 8 μm rat intestine section. A transverse section through the crypts shows proliferating cell locally abundant near the base of the crypts and diminishing with distance. Proliferating cells (brown) signal is visible against Movat’s Pentachrome staining consisting of Verhoeff-Van Gieson’s staining of elastin (black) and nuclei (purplish gray), alcoholic saffron staining of collagen (yellow), alcian blue staining of mucins marking the goblet cells (blue), and crocein scarlet–acid fuchsin staining of muscle and red blood cells (red). Image was acquired with a 10X objective using the RGB-mode illumination on an EVOS M5000 microscope.
Click image to enlarge
Figure 2. Differentiated mouse embryonic stem cells. The image shows mouse embryonic stem cells that have spontaneously differentiated into the three germ layers. The ectoderm was stained using anti-TUJ1 antibody, and the mesoderm was stained using anti-SMA antibody. Staining was done using secondary antibodies labeled with Alexa Fluor 488 and 555, respectively, and nuclei were stained using DAPI. Images were acquired at 10X using an EVOS M5000 microscope.
Click image to enlarge
Figure 3. Z-stack of honey bee claw imaged with EVOS M5000. MIP projection of an 11-slice image Z-stack of a honey bee claw captured using the EVOS 5000 microscope in color mode with a 10x apo objective.
Click image to enlarge
Figure 4. Transgenic fluorescent zebrafish (Fli1–enhanced Green Fluorescent Protein) including chorion, 24 hours post fertilization. Embryos were fixed in 4% paraformaldehyde and then imaged in PBS using the EVOS M5000 microscope with a 4x phase objective. Phase image including GFP overlay is shown.
EVOS M5000 – 3D deconvolution and analysis. HeLa Cells were labeled with ViewRNA probes (FITC against B2M and Cy5 against RPL13). Following counter-staining with DAPI, Z-stacks were acquired on an EVOS M5000 microscope with a 100x oil immersion objective. Image stacks were deconvolved using the 3D deconvolution module in Celleste 5.0 Image Analysis software. The animation was also generated in Celleste 5.0 following creation of iso surfaces and clipping planes.
EVOS M5000 Imaging System Specifications
| Optics | Infinity corrected optical system; RMS threaded objectives with 45 mm parfocal distance |
|---|---|
| Illumination | Adjustable intensity LED (>50,000 hour life per light cube); broad selection of patented standard and specialty light cubes |
| Light cubes (not included) | Commonly used light cubes include (Ex/Em):
|
| Contrast methods | Epifluorescence, transmitted light (brightfield and phase contrast) and RGB color illumination |
| Objective turret | 5 position; front mounted control |
| Objectives (not included) | Wide selection of high quality long working distance and coverslip corrected objectives |
| Condenser | 60-mm long working distance condenser; 4-position turret with a clear aperture and 3 phase annuli |
| Focus mechanism | Automated focus with sub micron (0.150 um) resolution (single step accuracy) |
| Mechanical focus wheel with single knob for coarse and fine focus | |
| Stage | Mechanical X/Y stage; travel range 120 mm x 80 mm with sub micron resolution, drop in inserts to receive vessel holders |
| LCD display | 18.5" articulated LCD color monitor with 1920x1080 pixel resolution |
| Camera | High-sensitivity 3.2 MP (2048 x 1536) monochrome CMOS sensor with 3.45 µm pixel resolution |
| Computer | Embedded PC with 4 GB RAM |
| Captured images | 16 bit monochrome TIFF or PNG (12 bit dynamic range); 8 bit color TIFF, PNG JPG and BMP |
| Storage | 10 Gb SSD |
| 16 Gb USB 3.0 memory stick | |
| Output ports | Power, 4 USB 2.0 ports, 1 USB 3.0 port, 1 Display Port, 1 RJ45 network jack |
| Networking capability | Connection through Windows/SMB network via an Ethernet cable connection |
| USB 3.0 WiFi dongle | |
| Power supply | Universal Power Supply (12V, 5A) with Type B (North America) power cord* |
| Dimensions (W x H x D) | 18 x 23 x 18 inches |
| Weight | 36 lb |
| * Note that one (1) country-specific power cord must be ordered separately in regions not using the Type B (North America) power plug: AMEP4646 EVOS Power Cord, Type C (Europe) AMEP4708 EVOS Power Cord, Type D (India/South Africa) AMEP4645 EVOS Power Cord, Type G (United Kingdom) AMEP4649 EVOS Power Cord, Type H (Israel) AMEP4647 EVOS Power Cord, Type I (Australia) AMEP4782 Power Cord, Type I (China, CCC certified) AMEP4786 Power Cord Type J (Switzerland) AMEP4648 EVOS Power Cord, Type L (Italy) | |
Stem cell biology: EVOS M5000 microscope at the cutting edge research
Cardiac repair and heart failure: EVOS M5000 microscope at the cutting-edge research
Liver regeneration: EVOS M5000 microscope at the cutting-edge research
Ordering information
Resources
EVOS Accessories
Cell Analysis Learning Center—Access cell analysis application resources for more success as you plan and execute your experiments.
Service
We provide a full range of service options for all EVOS cell imaging systems.
Support
Cell Analysis Support Center—Find tips, troubleshooting help, and resources for your cell analysis workflows.
For Research Use Only. Not for use in diagnostic procedures.